Lysis

Test tube 1 contained only 200 μL of Buffer and 50 μL of sheep erythrocytes, of which an additional 50 μL of erythrocytes were added before centrifuging. However, Test tube 8 was of a similar composition except in place of 200 μL of buffer, 200 μL of water were added to the solution. The lysis buffer’s function is to lyse the cell, however, in Test tube 1, without the presence of the appropriate complement the buffer could not break the membrane structure. Therefore this test tube demonstrates the need for compliment in cell lysis, even in the presence of the buffer’s detergents. In Test tube 8 water causes erythrocytes to swell and burst and is an excellent detergent, since it is hypotonic relative to the erythrocyte. The cells will thus swell. Test tube 8 provides an example of complete cell lysis where 100% of erythrocytes have burst, allowing for relative absorbance and percent lysis values to be calculated in contrast to complete lysis. These tubes control for the variance in the amount of cells lysed and the amount of red hemoglobin released from lysed cells, by comparing and contrasting the visual appearance and absorbance values of Test tube 1 and 8 against the…

Objective: We started by isolating a DNA plasmid from a bacterial culture for cloning. Then, we reverse transcribed mRNA to amplify it in a polymerase chain reaction. Finally, we amplified a specific gene (actin) through PCR. Protocol: A. Isolation of Plasmid DNA from a Bacterial Culture: We were provided with a tube containing 1 ml of bacterial culture. We centrifuged it for 5 minutes at 10000 g. Then, we removed the supernatant by pouring it off and we used a small chem wipe to remove any…

Preparation for Cell Lysis Obtain the mammalian expression vector for homo sapien ESR2 from Addgene. Assemble the vector to code for the proteins snail, slug and twist. Transfect HEK293 cells with the modified plasmids in petri dishes. Allow the cells to grow for 1-2 days so they will express the proteins. Transfer the culture medium to a centrifuge tube to separate it from the cells. Add 1mL of phosphate buffered saline (PBS) to the cells and swirl to mix. Separate the PBS from the cells and…

Socrates told Lysis “your father isn’t waiting for you to come of age before he trusts you with everything; but come the day when he thinks that you know more than he does, he’ll trust you with himself and everything that belongs to him” (Lysis, 209c-d). He was stating that one is not useful based on age, but chiefly on knowledge, and this skill can lead to greater things. I agree with this quote because of a personal anecdote. When I was a Boy Scout, I was the youngest to earn the Eagle Scout…

PURPOSE The purpose of this lab was to isolate DNA from a strawberry. METHODS We used a variety of materials to extract the DNA from the strawberry. These include a disposable cup, a rubber band, a plastic knife, a zipper bag, a pipet, a coffee filter, ethanol, a lysis buffer, a test tube, an inoculating loop, and a strawberry. The general objects that we used for convenience and safety include a lab coat, a tray to cut the strawberry, and lots of paper towels. The…

Plato talks about friendship directly in Lysis, however, Eros is a big part of how love and affection are shared in Plato’s ideology. Eros is translated as erotic love, and this is a range of how love is shown. While never directly stated in any of his dialogs, it can be interpreted that Eros includes friendship. Plato through Socrates discusses in a couple of dialogs (Symposium and Phaedrus) the ideas of beauty, sexual attraction, and love as a part of Eros. For Plato, these concepts are all…

Replenishing blood volume with distilled water will have disastrous effects on the body just because distilled water is very hypotonic to cells. Yes, viruses and bacteria are important considerations, but so is the outcome of cell lysis due to massive osmosis into the cell if a patient is given hypotonic…

equipment, the result DNA would become faulty or poor quality for further investigation My hypothesis predicts that if we were to use improper or non-lab chemicals, the result DNA would become faulty and would result in an inadequate samples for further studies, it would seem that if we were to extract DNA without chemicals like lysis solution, concentrated salt solution, resuspenstion buffer, ethanol or isopropyl alcohol, it would result in poor DNA results. Method one Method one to create a…

formol saline, the layer was detected of the pork liver internal side. There are red color layer and pink color layer. Red color meaning the formol saline not fully penetrate in the liver therefore it look like before the fixation.Also the pink side and pink surface is affected by formol saline but causing the weak RBC lysis power of formol saline, so it still look pink. the hardness is between the liver processing with acetic acid and processing with 3%potassium dichromate. biggest area of…

Due to limited time constraints and having lab only once a week this lab was broken up into five periods, the last one specifically to go over the results. Day one consisted of extraction of plasmid DNA, which we used Resuspension Buffer to break up our pellet after removing the supernatant (liquid) after centrifuging our overnight culture. Then Lysis Buffer was used to break down the membrane of the cells so that we could then add Neutralization Buffer to bring our sample from basic to neutral…