Zika Virus Lab Report

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Detection of Zika virus

Introduction: Zika Virus, also referred to as ZIKV, has become a global health epidemic. The World Health Organization declared a Public Health Emergency of International Concern earlier this year due to the increase of areas affected by Zika. Originating in the Zika forest of Uganda, ZIKV was predominantly found in areas of Africa and Asia. However, there have recently been reports of more than 36 countries with cases of the virus. (Chen, 1), (CDC)
ZIKV is a single stranded RNA virus that was originally found in a rhesus monkey. Transmission of the virus can happen through two types of modes. The first mode of transmission is through a bite from an infected Aedes mosquito. These mosquitos are considered to be carriers
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These samples were mixed with primers and probes and the resulted data were used to create calibration curves with different known concentrations. The serum samples detected two viruses; the Dengue fever and Chikungunya. The results from the test were negative, which indicates that there was no cross reactivity between the two viruses and the Zika virus. Four out of the six patient’s serum samples tested positive for the virus and two tested positive from the urine samples. However, urine sample showed more viral RNA concentration than the serum. (Gourinat, …show more content…
"Detection of Zika Virus in Urine." Emerg. Infect. Dis. Emerging Infectious Diseases 21.1 (2015): 84-86.

Faye, Oumar, Ousmane Faye, Diawo Diallo, Mawlouth Diallo, Manfred Weidmann, and Amadou Sall. "Quantitative Real-time PCR Detection of Zika Virus and Evaluation with Field-caught Mosquitoes." Virology Journal Virol J 10.1 (2013): 311.

Secondary

Wong, Marisa, and Juan Medrano. "Real-time PCR for MRNA Quantitation." BioTechniques Biotech. 39.1 (2005): 75-85.

Chen, Hui-Lan, and Ren-Bin Tang. "Why Zika Virus Infection Has Become a Public Health Concern?" Journal of the Chinese Medical Association79.4 (2016): 174-78.

Website

Disease." Centers for Disease Control and Prevention. Centers for Disease Control and Prevention, 2016. Web. 18 Apr. 2016.

"Essentials of Real-Time PCR." Thermo Fisher Scientific. N.p., n.d. Web. 17 Apr. 2016"About Zika Virus

"Symptoms, Diagnosis, & Treatment." Centers for Disease Control and Prevention. Centers for Disease Control and Prevention, 2016. Web. 18 Apr. 2016.

"Transmission & Risks." Centers for Disease Control and Prevention. Centers for Disease Control and Prevention, 2016. Web. 18 Apr.

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