Mycobacterium smegmatis

supernatant.The supernatant was filtered through a .22 micron filter in order to extract phages. Since bacteria and other particles tend to be bigger than .22 microns, they would not fit through the filter, thus allowing only phages through. Mycobacterium smegmatis was then added to the supernatant in the amount of .5 mL. It was allowed to incubate for 5 days. After incubating, 1.4 mL of the enriched filtrate was pipetted into a microcentrifuge tube. This was done twice using the p1000. Both tubes were then centrifuged for 1 minute to separate the pellet and the supernatant. The supernatant, 1 mL from each tube (2 mL in total), was then filtered through a .22 micron filter in order to retrieve phages. A petri dish was divided in four sections and labeled as positive control,…

infectious disease mainly in livestock caused by Mycobacterium bovis and has had substantial economic impacts in the UK and worldwide. The primary organ targeted by M.bovis is the host lung and the bacterial infection is accompanied by a localized inflammation, resulting in the migration of different immune cells and leakage of serum proteins on the site of infection. Some of the first host molecules to be involved are collectins, which are soluble innate immune molecules that play an important…

The Cause and Effects of Tuberculosis What are the cause and effects of tuberculosis? Tuberculosis is a disease caused by a bacterium. These bacteria mostly infect the lungs, but also they can infect the other parts of the body like the brain, spine, and kidney. (1,4) If the tuberculosis bacteria are active, they will cause the death of the tissue of organs that have been infected. Tuberculosis is one of a few diseases that "have caused so much distressing illness for centuries and claimed so…

synthase also has other targets that could be used as well such as the metal ion it binds to activate it. ATP synthase requires a proton gradient between inside and outside the membrane, therefore another target, which would not compete with a binding site. However the difficulty of a target such as this is, the specificity of any drug administered. As The mycobacterial ATP synthase bares much similarity with human ATP synthase, any therapies would need to be able to distinguish between human…

CHAPTER 1 INTRODUCTION 1.1 TUBERCULOSIS Tuberculosis (TB) is an infectious disease caused by the bacillus Mycobacterium tuberculosis. TB is a major global health problem and it ranks alongside Human Immunodeficiency Virus (HIV) as a leading cause of death worldwide. According to the estimates of WHO global tuberculosis report of 2015, there were 9.6 million people who developed TB and 1.5 million who died from the disease in 2014. 1.2 TRANSMISSION AND PATHOGENESIS OF TB TB infection occurs when…

Design and Procedures: The examination of the population incorporated all verified incident cases of TB reported to the Centers for Disease Control and Prevention's National Tuberculosis Surveillance System (NTSS) for people dwelling in selected US urban communities from 2000 through 2007. Case reports including the demographic, clinical, and treatment data of patients with TB were submitted to NTSS by the local and state health departments. Cases of TB were considered to occur in a selected city…

Name: Chelsy Oubre Causative Agent: Mycobacterium tuberculosis Disease: Tuberculosis (TB) Classification of the causative agent: Mycobacterium tuberculosis is an Acid-fast Gram-positive bacteria History: M. tuberculosis is the leading cause of death with people that are infected with HIV. TB kills a 5000 people on a daily basis. Virulence factors of the causative agent: Mycobacterium tuberculosis enters the body then continues to the lungs. The bacteria while in the alveolar…

When I began researching Mycobacterium leprae I knew very little about Leprosy and the information that I thought I knew was actually pretty inaccurate. There are a lot of rumors and theories about the disease and the actual route of transmission. If you are ever unfortunate to be plagued with this disease know that with the proper treatment and handled in a timely matter the long lasting effects will be kept to a minimum. This bacterium is an endospore and can stay dormant until the opportunity…

standing up inflicted pain. The patient did not recall any instance in which she strained or injured her spine lifting or any recent coughing episodes. The patient denies having a fever but stated she has had chills and headaches daily for at least a week. Patient stated she usually has intermittent headaches but recent headaches have been more severe and frequent. Patient denies any chest pain, shortness of breath, abdominal pain, nausea, vomiting, diarrhea, or constipation. There was no…

infection, Mtb primarily resides in macrophages, which, normally, destroy any phagocytosed organisms. However, Mtb is able to evade macrophage killing through the use of its type VII secretion system (T7SS)8. For Gram-negative bacteria, many stress response and virulence pathways (e.g. T7SS) are controlled by dimeric proteins called H-NS9, 10. How about Gram-positive bacteria like Mtb, does it have a similar protein? This was a mystery until Dr. Liu's publication in 2010. Dr. Liu and his…