supernatant.The supernatant was filtered through a .22 micron filter in order to extract phages. Since bacteria and other particles tend to be bigger than .22 microns, they would not fit through the filter, thus allowing only phages through. Mycobacterium smegmatis was then added to the supernatant in the amount of .5 mL. It was allowed to incubate for 5 days. After incubating, 1.4 mL of the enriched filtrate was pipetted into a microcentrifuge tube. This was done twice using the p1000. Both tubes were then centrifuged for 1 minute to separate the pellet and the supernatant. The supernatant, 1 mL from each tube (2 mL in total), was then filtered through a .22 micron filter in order to retrieve phages. A petri dish was divided in four sections and labeled as positive control,…
The Cause and Effects of Tuberculosis
What are the cause and effects of tuberculosis? Tuberculosis is a disease caused by a bacterium. These bacteria mostly infect the lungs, but also they can infect the other parts of the body like the brain, spine, and kidney. (1,4) If the tuberculosis bacteria are active, they will cause the death of the tissue of organs that have been infected. Tuberculosis is one of a few diseases that "have caused so much distressing illness for centuries and claimed so…
Tuberculosis (TB) is an infectious disease caused by the bacillus Mycobacterium tuberculosis. TB is a major global health problem and it ranks alongside Human Immunodeficiency Virus (HIV) as a leading cause of death worldwide. According to the estimates of WHO global tuberculosis report of 2015, there were 9.6 million people who developed TB and 1.5 million who died from the disease in 2014.
1.2 TRANSMISSION AND PATHOGENESIS OF TB
TB infection occurs when…
Sim Deep Non-motile
Negative H2s production
Litmus milk Acid /No clot
Manitol Salt Agar Acid/Positive
EMB Plate Ferments Lactose
These test below were left out of the identification process because the qualities that the organism pertained ruled them out. A spore stain determines if the organism is capable of producing spores. The class only had one spore forming…
top of the white dotes. After taking notes on the physical appearance of the bacteria’s, a gram stain was conducted on the isolated bacterial sample. The stain process is extremely essential because it separates the bacteria in two groups of gram positive and negatives bacteria’s .in addition, the gram stain shows the color, shape, size, and morphology of the bacteria. In order to narrow our search for unknown microbe, acid fast, spore and capsule stain were performed.
The spore stain…
electrophoresis. Then add 5 µl of concentrated 6x loading dye to each 25 µl restriction enzyme sample. Then load the gel in this order: ladder, Uncut DNA, BamHI, Clal, EcoRI, Haelll, HIndIII, Sall. Next, is to load the gel with the right amount of volume of DNA ladder. Use micropipettor for each sample (pipette 20 µl) of each RE reaction into the wells in order. Then plug the electrodes into the appropriate locations on the power supply. Turn power on and and set voltage to 100 V. Make sure to…