N-terminus

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  • Tpk-Trypsin Lab Report

    expect to see a reduction in the viral titer in the DF-1 cells that are stably expressing viperin compared to the DF-1 cells that are transfected with an empty vector. Objective 4: Identification of the critical regions and residues important for duck viperin function and localization. Viperin consists of three distinct domains; an N-terminal domain that varies considerably between species and contains both an amphipathic helix and a leucine zipper domain; the N-terminal domain plays an important role in binding to the cytosolic face of the ER, as well as its association with lipid droplets (Hinson & Cresswell 2009), the C-terminal domain of viperin is highly conserved, and its presence is critical for its antiviral function against the Flaviviridae members hepatitis C virus (HCV) and dengue virus (DENV) the center domain is also conservative between species, this domain has been shown to be important in viperin ability to limit both human immunodeficiency virus (HIV) (Nasr et al. 2012) and Bunyamwera virus (Carlton-Smith & Elliott 2012). In order to determine which of these three domains is essential to the viperin antiviral effect, Short and long N-terminal and C-terminal truncation mutants will be constructed, as well as mutations to SAM motifs in the central domain. These mutants will be used to evaluate the antiviral effect as well as the localization within the cells. I expect to see a major decline in the antiviral role with the mutations in the SAM motifs in the…

    Words: 1819 - Pages: 8
  • TM Landry Influenced My Life

    What impact has TM Landry had on me? An impact is the way an outside force influences an individual’s behavior. Everyone has experienced an event that has shaped their beliefs or influenced their actions. These events can vary from a death to moving schools. This was the case with Sean. Sean waited at the bus stop as he replayed the last two weeks. Sean’s dad had recently moved them to a different state for his new job. Since Sean was an introvert, he dreaded the idea of starting over in…

    Words: 748 - Pages: 3
  • Personal Narrative: Moving To Colorado

    Colorado a place where there are beautiful snowy mountains and a bunch of scenery. A girl from Los Angeles, California that would be the weirdest thing to imagine for someone who grew up around beaches and warm weather all the time. That girl was me, I just learned that I was moving from sunny California to bi-polar weather Colorado. Learning that I was moving to a completely different state was one of the hardest and weirdest things for me. During the months of September and October my…

    Words: 1184 - Pages: 5
  • Narrative Essay On Skytrain

    I had got use to having the skytrain doors shut in my face. No matter how confused or scared someone looked, no one had the time to stop or help a lost person. The ride back home seemed to be longer than usual and I could not help but notice the nervous group of kids trying to figure out the directions. Looking at them reminded me of the time when I first used the skytrain without any adult supervision. I vividly remember the struggle my little cousin and I faced just to get back home. It…

    Words: 1172 - Pages: 5
  • Summary Of Mayan History By Clendinnen

    Clendinnen recounts the history of the Yucatan peninsula once the Spanish arrived. She splits her recounting into two sections: the Spanish’s perceptive and the Mayan’s perspective. Clendinnen’s recounting the Spanish side of history demonstrates a struggle not only between the Spanish and the new land and its inhabitants, but also the internal conflicts between the Spanish settlers and the friars. At first she tells us how the Spaniards’ interactions with the natives consisted of tribute…

    Words: 746 - Pages: 3
  • Born Ready By Frida Kahlo Analysis

    about the difficulties she went through from being a young middle schooler to attending college at Harvard. She tells various situations of having been raised in Washington, D.C. and later on moving to Hawaii. At the beginning of the article Lawrence is riding home from school on a bus when a white man boards it. He is the only white person on a bus full of melanin and yet he feels the need to say the n-word. The aftermath was unbearable for young Kimiko, she wrote, “As I walk home from the bus…

    Words: 1724 - Pages: 7
  • Bloom And Borges Sparknotes

    Moreover, while Bloom endorses the linearity of influence, and Borges highlights its fluidity, a third distinct perception emerges. Thompson (2014, p.114) utilises David Foster Wallace’s short story B.I. #59 as a framework from which to interrogate Bloom and Borges’ arguments, detecting, “throughout [Wallace’s] fiction, influence comes not only from the past (as in Bloom’s model), or from a future, anticipated text (as in Borges’s model) but also from the cultural present.” Consequently,…

    Words: 1411 - Pages: 6
  • Personal Narrative: Wrong Time In Mexico

    Have you ever heard the saying, “wrong place, wrong time,” or maybe that life is about “being at the right place at the right time”? Or have you ever heard about the cartels and their crimes down in Mexico? Murders, kidnapping, drug trafficking, and lots of partying goes on every day by every cartel and by every drug lord south of the border. Unfortunately, these two topics have much in common in Mexico. Civilians that do not have anything to do with the cartels get caught up in these kind of…

    Words: 1170 - Pages: 5
  • Classic Maya Collapse Essay

    Climate Change and Trade Networks as Causes of the Classic Maya Collapse The ancient Lowland Maya were a thriving and advanced society, capable of building great monuments able to survive to the present. It is therefore a mystery as to why, at the end of the Classic period from the eighth to eleventh century, Maya sites show signs of massive decline and desertion; this is referred to as the “collapse” of the Maya, though it was not an immediate or evenly distributed phenomenon (Douglas,…

    Words: 1544 - Pages: 7
  • The Homestead On Rainy Creek By N. Scott Momaday Analysis

    "The Homestead on Rainy Mountain Creek," written by N. Scott Momaday, is a memoir about the author 's childhood and ancestral history in the Kiowa village of Rainy Mountain Creek. He speaks about the various traditions of the Kiowa tribe, the preservation of memory, the geography of the "mountain", the importance of family, and the traditional values of the tribe versus the invading european "white" culture. However, I believe that the main focus of this memoir is the Kiowa Tribe itself and its…

    Words: 1075 - Pages: 4
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