Enterobacteriaceae

Based on the dichotomous key and series of test that was used to conduct this lab project, the biochemical tests for enteric bacteria indicated that the unknown bacterium #63 being a Citrobacter freundii (218) due to commonly found in the gastrointestinal tract and known to have the ability to ferment lactose to produce acid and gas and with few exceptions a sulfur-reducing. Humans are unique species. Just like in the world they live in have different types of cities, race, and culture; The human body also have different types of normal flora and pathogenic bacteria inhabiting in different parts of the body especially the gastrointestinal tract and these organisms are referred to as enteric bacterium that are placed in the family Enterobacteriaceae. They are characterized by their ability to glucose/lactose fermenting, oxidase negative, facultative anaerobes and being a gram-negative bacilli (Tortora, Funke and Case 2010). The whole purpose of conducting the unknown lab for enteric bacteria is to apply the lab techniques I have learned and acquired, so that I can interpret those biochemical test results to identify the unknown enteric bacterium that was used for this project. The unknown bacteria used for this lab project were Pseudomonas aeruginosa, Serratia marcescens, Proteus mirabilis, Proteus vulgaris, Klebsiella pneumoniae, Enterobacter aerogenes, Escherichia coli, and Citrobacter freundii. In the world such as the medical field, biochemical test are used to diagnose…

Introduction Antibiotic resistance is a major threat to the health and wellbeing of the population. Antibiotic resistance has posed challenges to the public health community, while negatively impacting the general population (Another sentence of two about antibiotic resistance in general, the increasing trends and some figures). Today, carbapenemase producing-carbapenem resistant Enterobacteriaceae (CP-CRE) is a group of gram-negative bacteria that are challenging public health professionals…

officer Dame Sally Davies has even made the argument that antibiotic resistant disease should be considered a "national emergency", putting it in the same category as terrorism and natural disasters. Many of us have heard of the more common "superbugs". MRSA is by far the most common one that we hear of floating around long-term care facilities and hospitals. It turns out that a relatively new bug has also come on the scene. Carbapenem-resistant Enterobacteriaceae, more commonly referred to as…

The properties of selective and differential media suggest that differences between the bacterial species could be used in this lab to identify these six species but their effectiveness is unknown. The efficacy of identifying a completely unidentified bacterium in a cost-sensitive manner using these test also has yet to be determined. We hypothesized that applying a combination of selective and differential media with biochemical tests would provide a presumptive identification of the first…

no LPOS (T-1) 3. Coating formulation: 2 % alginate-LPOS (T-2) 4. Coating formulation: 4 % alginate-LPOS (T-4) 5. Coating formulation: 6 % alginate-LPOS (T-6) For each segment, two fillets were used for every test day. Therefore for days 0, 4, 8, 12 and 16. Ten samples for each lot were evaluated. So the total of 60 samples were analyzed. Evolution of the microbial spoilage The effect of alginate and alginate-LPOS coating in different levels of 2, 4, and 6% over the microbial…

The scientific context of the Antimicrobial Susceptibility test is to gauge the viability of antimicrobials and other chemotherapeutic agents on pathogenic organisms in recommending a suitable treatment. The scientific context of the the API 20E test is clinically used in quickly identifying enterobacteriaceae bacteria and gram-negative rods. In the Antimicrobial Susceptibility experiment, three microorganisms which were Staphylococcus aureus, Escherichia coli, and the unknown were put into…

Citrate: The citrate test gave a positive result, showing a dark blue color in the tube, caused by the alkaline conditions present, which is caused by the bacteria’s ability to utilize the citric acid present in the citrate slant. The positive result confirms the presecnce of the citrase enzyme. H2S Production: The H2S test yielded a positive result, giving rise to a black precipitate created by iron and H2S combining. Proving the presence of the desulfurase enzyme. Indole: The indole test…

been instantly cured by penicillin in the early twentieth century, but in the present day, the infection needed more complicated courses and managed the different kinds of drugs to be treated. The other disease, “MRSA, methicillin-resistant Staphylococcus aureus, was a type of staph that resisted the antibiotics called beta-lactams.” The infection often began with a little cut, which got infected with bacteria. Staphylococcus aureus combated the penicillin and “the most minor cuts in the skin…

reaction the organism has. Next a sample of the mixed broth is taken and cultured on a nutrient agar plate using streak plate technique to isolate the Enterobacteriaceae and then the plate is incubated at 35C. The plate then checked after 48 hours for isolation, and then a sample is taken and checked using a gram stain method to see if the organisms have been isolated. If the colony sampled had only one type of bacteria and it is gram negative, then re-streak plate the colony on a new nutrient…

“The ompk6proein is created by the Enterobacteriaceae group of bacteria and has a wide range of harmful inflammatory effects on the body,” Carducci explained. “And yet all mice who had been vaccinated showed almost normal levels of blood sugar and lower levels of insulin in spite of their diet,” Carducci explained. High blood sugar and high insulin are two of the most important risk factors in the development of type two diabetes, the main causes of which are thought to be a diet high in sugar…