Broth

bacterium following Bergey’s Manual, a broth, a deep, an agar slant and a streak plate are all inoculated with the unknown bacterium via a needle for the deep and a loop for the broth, agar slant and streak plate. After forty-eight hours all the mediums are examined to see what growth has occurred and the characteristics of growth (if any) are documented. This bacterium was found to be motile from results via the deep, a thin film was visible atop the broth, and sediment was seen at the bottom,…

grew throughout all of the thioglycollate broth, but there was more growth towards the bottom. The aerobic TSA + glucose plate did not have any growth, but the anaerobic plate did have growth. Escherichia coli grew throughout all of the thioglycollate broth as well, but the organism was clustered slightly more towards the top. There was growth in both aerobic and anaerobic TSA + glucose plates. Micrococcus luteus only grew at the top of the thioglycollate broth and it grew in the aerobic TSA +…

with an egg pasta, but what you fill it with varies from family to family. People pack it with ricotta, parmesan, prosciutto, or beef and sometimes it's a combination of meat and cheese. No matter what the filling, it’s traditionally served in a broth. Tortellini is not to be confused with…

- AN IN-VITRO STUDY” INTRODUCTION Medical microbiology entered a new era towards the end of the nineteenth century. It was during these golden years that path-breaking work of Pasteur, Koch and others in the development of broth and agar media led to the ability to grow a majority of pathogenic micro-organisms which were deemed uncultivable till then. In 1894, WD Miller published his findings on the bacteriological investigation of pulps. The results of past studies have generally…

Van Tran Microbiology Tuesday 5:00 May 5, 2015 Unknown Sample # 7 1. Introduction: Using biochemical tests are a good way to identify unknown bacteria from a sample. It is basically a similar to a trial and error method, but rather than using trial and error a person would be using positive and negative results. Using biochemical tests will help a person identify certain characteristics of the unknown bacteria through a series of chemical tests. Of course more than one biochemical tests…

¼ tsp. salt ½ tsp. pepper ¾ cup low sodium beef broth INSTRUCTIONS 1. Preheat an oven to 250°F then coat a casserole dish with cooking spray. 2. Combine ground beef with eggs and minced garlic then shape into small balls forms. 3. Arrange the balls in the casserole dish then set aside. 4. Preheat a saucepan over medium heat then pour olive oil into it. 5. Stir in chopped onion then sauté until translucent and aromatic. 6. Pour low sodium beef broth into the saucepan then…

Once the meat is finished, shred the meat using two forks, and reserve some of the broth for an additional step. On day two, simmer the cooked meat on the stove while prepping the remaining recipe items. Next, soak the corn husks for at least thirty minutes in hot water. It is ideal to purchase precut corn husks also from a local grocery…

combinations of LB broth, ampicillin, and arabinose. The second indicator was fluorescence, which was observed under UV light. The presence of fluorescence indicated that the bacteria had taken up and expressed the pGLO plasmid, which contained the GFP gene that produces green fluorescent protein. The four plates used in the experiment were the +pGLO LB/AMP/ARA plate, the -pGLO LB/AMP plate, the +pGLO LB/AMP plate, and the -pGLO LB plate. The +pGLO LB/AMP/ARA plate contained LB broth,…

I. Purpose: The purpose of this lab was to learn how to properly mount a bacteria for examination under a microscope. Also learned in this lab were methods on how to inoculate an agar or broth medium, and using aseptic techniques. Lastly, how to use stains to visualize bacterial cells, or distinguish different types of cells from one another through the usage of dye techniques such as the Gram-stain test was learned. II. Prepping/Mounting/Simple Staining Bacterial Cells Procedure: 1. Obtain 3…

Results: In order to identify the gram positive isolate in the unknown #260 broth, I observed the bacterium under the microscope, created a stock culture, and conducted a variety of biochemical tests. First, I prepared a gram stain of the mixed culture using aseptic technique, shown in Figure 1, and found two different bacteria. There were too numerous to count purple cocci in clusters, pairs, and some single cells, which I identified as the target isolate. The other bacteria present a pink…