Broth

from a mixed broth culture. Multiple tests will be performed in order to eliminate all organisms from the list that do not provide significant results for the identification of the unknown specimens. Journal Entries: April 17, 2018. Today we began the unknown project. I obtained the unknown broth tube #15. I used two Tryptic Soy Agar (TSA) plates in order to make a streak plate. The streak plate is used to separate the two different bacterial organisms that were present in the broth. The…

9.3. Lauryl tryptose broth (MERCK) Tryptose ..................................................................................................................... 20.0 g Lactose.........................................................................................................................5.0 g Dipotassium hydrogen phosphate, K2HPO4 ..............................................................2.75 g Potassium dihydrogen phosphate, KH2PO4..................................................…

What can be a better way of celebrating the Homemade Soup Day than with a bowl of sizzling chicken soup? This Homemade Soup Day, relish your soups and let your body reap in the benefits that a bowl of veggie or meat soup provides. Soups, be it vegetable soup or chicken soup, are essentially healthy as it is packed with the goodness of vegetables and is cooked minus oil. And, the health benefit of chicken soup, especially for common cold, has been known since ancient times and considered a…

In medical or science field, biochemical test are used to identify unknown bacteria. In this case three test are essential to identify the unknown #63 and they are MAC agar test, BCP (lactose) broth and SIM deep medium/slant. Methods such as streak plate technique, aseptic technique, and proper handling of the microbe are necessary to apply when conducting these biochemical test. Contamination during the unknown lab project shows carelessness and may put patient in jeopardy due to incorrect…

INTRODUCTION Bacteria can be conserved in a lab in many different ways, but the most common way is a suspension culture. A suspension culture is when the cells are grown in a sterile, full of supplements, broth. The broth allows the cells to divide and grow easily and will later turn into broth turbid. When culturing microorganism, the culture has to be completely pure. The best technique to preserve the culture pure is by avoiding bacteria in the environment to contaminate the culture, and to…

seconds and then returned to ice. After two minutes on ice, we removed the tubes, pipetted 250 ul of LB broth to each tube, and then incubated them at room temperature for an additional 10 minutes. We prepared four agar plates; two plates contained LB broth and ampicillin [LB/amp], another plate contained LB broth, ampicillin, and arabinose [LB/amp/ara], and the last plate contained only LB broth [LB]. We pipetted 100 ul of transformed bacterial suspension to the center of an LB/amp plate and…

Results: In order to identify the gram negative isolate in the unknown #260 broth, I observed the bacterium under the microscope, created a stock culture, and conducted a variety of biochemical tests. First, I inoculated a blood agar plate and a MacConkey (MAC) agar plate with the unknown #260 mixed culture broth. The blood agar plate, shown in Figure 1, was inoculated using the quadrant streaking method and then incubated for 24 hours at 35 °C. In the third and fourth quadrants, two different…

Title: Discovering Microorganisms Inside of the Lab and Out Introduction: Microorganisms are everywhere, and they are usually associated with sickness or disease like E. Coli or Staphylococci. In reality, microorganisms help more than they harm. Microorganisms in our bodies help make everyday normal bodily functions possible. For example, normal microbiota found on the skin and gastrointestinal microbiota found in the digestive tract. If it was not present, it would make humans a lot more…

restaurant, or even the kind from the can, that people find enjoyable about soup. One may ask, what are the characteristics about soup that draws people to it? Soup is diverse in its appeals, simple or exotic in its ingredients, light or creamy in its broth, and casual or formal in its purpose. The chef is the ultimate decision maker in all these factors. A chef’s artistry is similar to that of a writer’s, or speaker’s being that the final product conveys the artist's signature style and aims to…

Hannah Noetzel Blk. 3 10/25/17 Bacterial Transformation Utilizing E. Coli Background Through this lab there were many terms used that may confuse you so before I begin explaining the lab, here’s some background information on the subject of biology. A plasmid is bacteria naturally containing one or more tiny circular pieces of DNA. The two genes being used in this experiment are Ampicillin and fluorescent genes. When reading about the lab, the most common thing used in…