Broth

surface, including the human body, will yield many different types of microorganisms. Once grown on a nutrient-rich medium, the identification of these microorganisms can be made by isolating pure colonies and performing and using various plates and broth and recording its characteristics. It is important to understand and characterize each microorganism, behavior, reaction, and appearance for future purposes. In this experiment, the identification of two unknown bacteria was…

results. Also incubate the agar slant at 37˚C for about 48 hours. The third test that was performed was a fermentation test for sucrose. Using the inoculating loop, transfer an inoculum of the suspected microbe into a phenol red sucrose broth. Mix the inoculum and the broth then incubate it within 48 hours at 37˚C. Urease test was the last biochemical test that was performed this day. A urea tablet was added using the tweezers to a small tube containing one mL of distilled water. After adding…

Inoculating loop, Nutrient broth tube Materials used in experiment 1.2B: Inoculating loop, Nutrient broth tube, Bunsen burner, Flint In experiment 1.2B the end of an inoculating loop was brushed against one’s finger. A nutrient broth tube was then…

onion chopped, 2 cloves of minced garlic, 2 table spoons of unsalted butter, ¼ cup of chicken broth, ½ cup of cooked honey turkey roughly chopped, 1 tablespoon of fresh parsley finely chopped, 1 tablespoon of Cajun seasoning, 1teaspoon of black pepper, 1 tablespoon of onion powder, 1 tablespoon of garlic powder. For the grits: 1 cup of uncooked quick-cooking grits, 1 cup of cheddar cheese, 1 cup of chicken broth, 2 cups…

to determine two bacteria in a given broth. For this experiment we were provided with an unknown broth, along with the supplies and media to perform the needed tests that would determine what bacteria the broth contained. We were also provided with a chart of possible bacteria. All tests were preformed with aseptic and sterile techniques. After being given the broth, using sterile methods I streaked a TSA plate using the quadrant method with the given broth, in hope to grow the bacteria in…

Then, we looked at the Tributyrin agar and looked for a clearing/halo around the bacteria. After this procedure, we inoculated three urea broths and after storing it at our optimal temperature for 48 hours we brought it out and examined them for color change. After these tests had been run and observed we grabbed three nutrient gelatin stabs, and we inoculated two tubes, this time we stabbed…

one-fourth of warm water in the pot then half of a gallon of sunny D which is added for a more flavorful taste to the broth. We will then need to add a ton of fire seasonings to this pot of broth. You will then need to add two to three, four inch jalapenos that are already cut up into pieces and ready to be tossed into the pot. That is going to be one of the main ingredients to a perfect broth. After that, it is essential to add half a bag of Zatarain's 5 lb crawfish seasoning which is required…

Discussion: Each test is a pertinent contribution to drawing an educated conclusion. After performing the gram staining and observing which unknown culture was gram positive, a catalase test was performed. The catalase test allows lab workers to differentiate Staphylococci and Streptococci genus. The test was performed only on the gram-positive culture. Bacteria that respire by the use of oxygen, otherwise known as aerobic respiration, produce enzyme or “catalase”. This protects the bacteria…

Coliscan easygel nutrient broth was stored in the freezer. Around 24 hours prior to sampling, the broth was moved to the refrigerator to thaw. The sampling procedure follows the procedure stated in the Coliscan Easygel Instructions. When we were ready to begin sampling, the thawed broth was placed into a cooler. We started sampling at Cornerstone pond then move to the Wyoming site, then downtown Grand Rapids, then Plainfield, and then Versluis lake. The procedure for Cornerstone pond and…

are grown in broth containing 1% 1M glucose, then they will divide more rapidly than they would without the glucose additive. Our independent variable was the glucose, and our dependent variable was E.coli cell density. Methods: This procedure was obtained from the Growth Kinetics in Bacterial Cell Culture in the Cell Biology Lab Manual of Carthage College (2016). The wavelength of the spectrophotometer was set to 600nm. Four flasks were obtained that contained 50mL bacteria broth and labeled…