Broth

will be discussed are Agar media and Broth media: the basic difference between the two is that Agar media is solid in state, thus meaning it is invasive (or does not allow oxygen to enter the environment), and that Broth media is liquid in state, which is a complete opposite of the Agar media (meaning it does allow oxygen to enter the environments). Considering this information, the conclusion can be drawn that eukaryotic cells will most likely grow in Broth media, due to the presence of oxygen,…

3.1. Growth Kinetic of strain: The growth curve of strain in both of mediums is shown in Fig. 1 (Supplementary data). It was apparent that 2 hours after nucleation of culture, strain interred to logarithmic phase and continues for 10 h in MRS broth and 12 h in PPM medium. Sonication continued in logarithmic phase in 2 h intervals. 3.2. The effect of ultrasound on growth kinetic: The growth curves plotted for the two condition (with and without sonication) (Fig. 1), growth kinetic parameters…

After testing the plasmids for the green fluorescent protein using a black light we concluded that plasmid 3 was ampicillin resistant, plasmid 2 was GFP/ Ampicillin resistant, and plasmid 1 was Kanamycin resistant. We saw lawns on all the Luria Broth…

1. Create a recipe following the guidelines from National Geographic Soup Challenge. My soup is ham and potato cheddar soup, the way you make it is, Combine potatoes, ham and water in a large pot. Bring to a boil, then cook on medium heat for 10-15 minutes (or when potatoes are tender). Add chicken bouillon cubes, salt and pepper. Mix. In another pan, melt butter on medium heat. Whisk in flour and stir constantly for about 1 minute. Slowly add milk and continue whisking until sauce thickens…

used to discover the identity of the given bacterial culture was the carbohydrate utilization tests. This specific set of tests involved two broths, namely phenol red lactose and phenol red sucrose, and a Durham tube placed upside down in the broth tubes. These broths can be broken down by fermentation processes, which produce an acid, gas or both. The broth tubes also contain a pH color indicator, which changes color to indicate if acid has been produced. The pH indicator will turn from a red…

filling the whole plate but it was much more than half the +LB/AMP plate. In any experiment there is always a chance of error, this lab was no exception. It is very likely that when we were transferring the Luria Broth and the plate there may have been a varied amount of the luria broth in in each plate because it was very hard to hit the prescribed mark and make sure there was no residue left over in the pipet. Also unwanted microbes could enter the plate if they were open for too long. If I…

Identifying a bacteria based off of physical characteristics is almost impossible due to the commonality of shapes and physical features. Because of this, a wide range of biochemical tests were preformed in order to better identify the bacteria. Being able to identify an unknown was the most interesting part of the microbiology lab this semester, as well as, being able to preform the various tests. Proper aseptic technique was used throughout the semester and followed during all biochemical…

Throughout the semester in the microbiology lab we have been taught how to conduct multiple experiments to identify microorganisms. Many of these experiments can be used on all different types of microorganisms but cumulatively they can help isolate a singular microorganism’s identity. At the beginning of the semester we were divided into several groups and were assigned an unknown of which we were tasked with identifying. Being assigned number 14 as our unknown, my group consisting of…

Sensitivity Tests 1. Dilution Method Broth Dilution: this method involves exposing isolates, to a series of concentrations of antimicrobial agents in a broth environment. For this method, the lowest concentration at which the isolate is completely inhibited is recorded as the minimal inhibitory concentration (MIC). The test is only valid if the positive control shows growth, and the negative control shows no growth. Agar Dilution: this method has the same procedure as the broth dilution. Agar…

Introduction: Klebsiella pneumoniae is a part of the family Enterobacteriaceae. This genus is a gram negative and it has no flagella, which meant that this bacterium is not motile. However, it can move with the use of the current that is produced by the water. This bacterium has the ability to cause destructive change in a man or animals lungs if aspirated. In addition, this is also one of the most contacted pathogen in a Hospital Acquired Infection (HAI) or nosocomial infection. In this…