+ Lb Lab Report

Conclusion My initial hypothesis was that the -LB plate will have no growth, the -LB/AMP with have a lawn of bacteria, the +LB/AMP plate would have a number of small colonies filling about half of the plate and the +LB/AMP/ARA plate would be filled with with colonies but not enough to create a lawn (approximately half of the +LB/AMP). Based on the data solely from our group my hypothesis is somewhat supported. The -LB plate had no growth, as predicted. The -LB/AMP plate had a lawn of bacteria making the plate translucent. The +LB/AMP plate barely had any colonies, after counting we got approximately thirteen colonies which was much less than I had predicted. The +LB/AMP/ARA plate met my prediction of filling the whole plate but it was much more than half the +LB/AMP plate. In any experiment there is always a chance of error, this lab was no exception. It is very likely that when we were transferring the Luria Broth and the plate there may have been a varied amount of the luria broth in in each plate because it was very hard to hit the prescribed mark and make sure there was no residue left over in the pipet. Also unwanted microbes could enter the plate if they were open for too long. If I were to do this lab a second time in order to get more successful and accurate …show more content…
that protein synthesis is the way the genetic code puts together proteins in the cell and gene expression is the characteristic or effect attributed to a particular gene also by completing this lab the concept of an inducible operon system became for clear. In the case of this lab adding an inducer, in this case the arabinose, would cause the repressor to become inactive, which allows the RNA Polymerase to enter and make the green fluorescent protein (GFP) through the process of protein