Throughout the semester in the microbiology lab we have been taught how to conduct multiple experiments to identify microorganisms. Many of these experiments can be used on all different types of microorganisms but cumulatively they can help isolate a singular microorganism’s identity. At the beginning of the semester we were divided into several groups and were assigned an unknown of which we were tasked with identifying. Being assigned number 14 as our unknown, my group consisting of Chijindu Eke, Austin Dicus and myself conducted these experiments over the course of the semester and have successfully identified our microorganism to be Enterobacter Aerogenes. The plan to identify the microorganism was to grow a pure and uncontaminated
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Then we also conducted the three in one experiment known as the SIM which stands for the Sulfide Indole Motility. In this experiment we prepared the agar by stabbing it with the microorganism and letting it grow. The results would tell us if our microorganism broke down certain amino acids and produces the byproducts and the growth out from the stabs to indicate the motility of the substance. After the addition of the reagent to detect the Indole production our specimen appeared to have experimented negative for hydrogen sulfide and Indole but experimented positive for Motility as shown by the growth out of the stabs. Then at this point I personally prepared a new batch of microorganism using aseptic technique to produce a lawn for the next sets of …show more content…
In which we checked for the transfer of electrons to cytochrome before it goes through the respiration process for aerobes. Our organism experimented negative for this experiment. This experiment concluded all of the identification experiments for our diagnostic review of the microorganism. This was when we combined the data for review.
After pooling all of our data together from the various experiments done aseptically, we developed a diagnostic key that helped us identify the microorganism through process of elimination. This ultimately helped us decide that our organism was Enterobacter Aerogenes. Out of the microorganism in our chart our unknown results matched with the results that were associated with this bacteria the
Then we also conducted the three in one experiment known as the SIM which stands for the Sulfide Indole Motility. In this experiment we prepared the agar by stabbing it with the microorganism and letting it grow. The results would tell us if our microorganism broke down certain amino acids and produces the byproducts and the growth out from the stabs to indicate the motility of the substance. After the addition of the reagent to detect the Indole production our specimen appeared to have experimented negative for hydrogen sulfide and Indole but experimented positive for Motility as shown by the growth out of the stabs. Then at this point I personally prepared a new batch of microorganism using aseptic technique to produce a lawn for the next sets of …show more content…
In which we checked for the transfer of electrons to cytochrome before it goes through the respiration process for aerobes. Our organism experimented negative for this experiment. This experiment concluded all of the identification experiments for our diagnostic review of the microorganism. This was when we combined the data for review.
After pooling all of our data together from the various experiments done aseptically, we developed a diagnostic key that helped us identify the microorganism through process of elimination. This ultimately helped us decide that our organism was Enterobacter Aerogenes. Out of the microorganism in our chart our unknown results matched with the results that were associated with this bacteria the