Transmission electron microscopy

existence has raised many controversial questions, including whether or they are alive. Some experts claim nanobes are simply too small to be life forms, while others assert that nanobes are most certainly alive. Although the evidence from experiments by Kajander and Ciftcioglu can be used to support both sides of the argument, I believe that there is a more viable case for the theory that nanobacteria are indeed alive. Nanobes are the smallest cell-walled organisms on earth. When scientists viewed the nanoparticles under electron microscopy, the particles appeared to have an outer layer or membrane that may represent a cell wall. This outer layer surrounds an electron dense region interpreted to be the cytoplasm and a less electron dense central region that may represent a nuclear area. The presence of a cell wall typically indicates signs of life because cell walls are essential for the survival of the cell. Furthermore, the TEM (transmission electron microscopy) of negatively stained biofilm material revealed small coccoid-shaped particles, which contributes to the cell-like appearance of nanobacteria. The nanobes looked strikingly similar to bacteria, forming spheres, chains of beads, filaments, or bean- or sausage-like shapes. In the same vein, when purified phosphotidyl inositol (a phospholipid common to biological membranes) was added to the culture, biofilm formation occurred within two weeks. The appearance of the particles was very akin to those found in…

3a–b. All the catalyst nanoparticles are uniformly distributed on the carbon support with a graphitic layer. Although the average particle size of Pt and PtFeS catalysts, obtained from more than 20 nanoparticles in their corresponding TEM images, is 3.12 and 3.37 nm, respectively, a clear difference in the morphology was observed. The TEM image of Pt catalyst shows spherical shaped Pt nanoparticles, in contrast to PtFeS catalyst exhibiting a mixture of spherical and rod-like morphology. The…

bezenethiol ligand exchanged CdSe@ZnS solutions. These solutions were centrifuged 12,000rpm for 10min to remove the by-product which are not soluble in hexane solvent. The resulting QDs solutions were purified thrice by precipitation and centrifugation with a solvent combination of hexane and anhydrous ethanol (1:4 volume ratio) by centrifugation (10,000 rpm, 10 min), Finally, the benzenethiol-capped QDs were dispersed in hexane at 10 mg/mL. Synthesis of ZnO NPs ZnO nanoparticles (NPs) were…

Transmission electron microscopy, or TEM, is a variation of low field, high-powered electron microscopy. Created by Ernest Ruska and Max Knoll of the Berlin Technische Hochschule in 1931, the invention of this novel microscope allowed for the surpassing of the visible light resolution barrier. Since its inception, the TEM has become a resolution standard in the field of imagery, and further advances have led to the progression of high quality atomic viewing. The development of the electron…

During the microscope lab, my partner and I learned how to properly use a microscope, calculate the field of view, and view and prepare slides. Microscopes are instruments used to view very small objects that cannot be seen with the naked eye. We found that microscopes have many different parts to them, including the diaphragm, ocular lens, and objective lenses. There are three powers of magnification, scanning power (4x), low power (10x), and high power (40x). To find the total magnification,…

During this lab, Sfr13 mutant and wild type Tetrahymena’s number of India Ink vacuoles and sizes were observed over multiple trials, and then the averages were compared to see if there was any difference between the mutant and WT. Furthermore, the two strains of Tetrahymena were stained with mCherry fluorescence, so that the oral grooves could be examined and compared under fluorescence microscopy. This experiment was designed to eliminate as much bias as possible by replicating the experiment…

endometrium of women who had endometriosis and those whom didn’t have it. The results of the two populations were compared. Due to this being done, it was shown that a particular protein known as DJ-1, is present and upregulated in eutopic endometrium of the woman surveyed with endometriosis. Therefore, it was inferred, that this protein may have impact or involvement of how endometriosis occurs. DJ-1 is a protein that is known to affect cell survival and growth as well as in ant oxidative…

This article discusses the research behind the bioluminescent millipedes. These rare species are classified as Motyxia. Paul Marek is an entomologist who was determined to find these rare millipedes near San Luis Obispo, California. Marek set out on his journey in 2013. Bioluminescent millipedes are fluorescent, meaning they glow a green hue under UV light. Marek found it easier to search for the millipedes during the night. Soon after arriving at his location he found three bioluminescent…

The slide used during the Microscope Lab was a wet-mount slide which was used in order to view the specimens from the microscope. A wet-mount is a method for preparing specimens to view using a microscope. A myriad of specimens look better when placed in a drop of water on the microscope slide. The water supports the sample and fills the space between the coverslip and the slide. This allows light to pass easily through the slide, the sample, and the coverslip. Scientists use the wet-mount…

Abstract- The main material and focus in this lab is the compound light microscope. During this lab you will check the microscopes by drawing what you see to make sure the high and low powers work properly. After you tested your microscope you take a toothpick and scrap it on the side of you mouth and examine what you see through the microscope, your final results should be a drawing of what you saw through both the high and low powered lens. Introduction- This lab is based on the…