Transmission electron microscopy

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  • Nanobacteria Research Paper

    existence has raised many controversial questions, including whether or they are alive. Some experts claim nanobes are simply too small to be life forms, while others assert that nanobes are most certainly alive. Although the evidence from experiments by Kajander and Ciftcioglu can be used to support both sides of the argument, I believe that there is a more viable case for the theory that nanobacteria are indeed alive. Nanobes are the smallest cell-walled organisms on earth. When scientists viewed the nanoparticles under electron microscopy, the particles appeared to have an outer layer or membrane that may represent a cell wall. This outer layer surrounds an electron dense region interpreted to be the cytoplasm and a less electron dense central region that may represent a nuclear area. The presence of a cell wall typically indicates signs of life because cell walls are essential for the survival of the cell. Furthermore, the TEM (transmission electron microscopy) of negatively stained biofilm material revealed small coccoid-shaped particles, which contributes to the cell-like appearance of nanobacteria. The nanobes looked strikingly similar to bacteria, forming spheres, chains of beads, filaments, or bean- or sausage-like shapes. In the same vein, when purified phosphotidyl inositol (a phospholipid common to biological membranes) was added to the culture, biofilm formation occurred within two weeks. The appearance of the particles was very akin to those found in…

    Words: 1171 - Pages: 5
  • Benzenethiol Ligand Study

    bezenethiol ligand exchanged CdSe@ZnS solutions. These solutions were centrifuged 12,000rpm for 10min to remove the by-product which are not soluble in hexane solvent. The resulting QDs solutions were purified thrice by precipitation and centrifugation with a solvent combination of hexane and anhydrous ethanol (1:4 volume ratio) by centrifugation (10,000 rpm, 10 min), Finally, the benzenethiol-capped QDs were dispersed in hexane at 10 mg/mL. Synthesis of ZnO NPs ZnO nanoparticles (NPs) were…

    Words: 985 - Pages: 4
  • Ptfe Lab Report

    3a–b. All the catalyst nanoparticles are uniformly distributed on the carbon support with a graphitic layer. Although the average particle size of Pt and PtFeS catalysts, obtained from more than 20 nanoparticles in their corresponding TEM images, is 3.12 and 3.37 nm, respectively, a clear difference in the morphology was observed. The TEM image of Pt catalyst shows spherical shaped Pt nanoparticles, in contrast to PtFeS catalyst exhibiting a mixture of spherical and rod-like morphology. The…

    Words: 1386 - Pages: 6
  • Electron Microscopy Essay

    Transmission electron microscopy, or TEM, is a variation of low field, high-powered electron microscopy. Created by Ernest Ruska and Max Knoll of the Berlin Technische Hochschule in 1931, the invention of this novel microscope allowed for the surpassing of the visible light resolution barrier. Since its inception, the TEM has become a resolution standard in the field of imagery, and further advances have led to the progression of high quality atomic viewing. The development of the electron…

    Words: 892 - Pages: 4
  • Microscope Lab Report Essay

    During the microscope lab, my partner and I learned how to properly use a microscope, calculate the field of view, and view and prepare slides. Microscopes are instruments used to view very small objects that cannot be seen with the naked eye. We found that microscopes have many different parts to them, including the diaphragm, ocular lens, and objective lenses. There are three powers of magnification, scanning power (4x), low power (10x), and high power (40x). To find the total magnification,…

    Words: 698 - Pages: 3
  • Tetrahymena Lab Report

    During this lab, Sfr13 mutant and wild type Tetrahymena’s number of India Ink vacuoles and sizes were observed over multiple trials, and then the averages were compared to see if there was any difference between the mutant and WT. Furthermore, the two strains of Tetrahymena were stained with mCherry fluorescence, so that the oral grooves could be examined and compared under fluorescence microscopy. This experiment was designed to eliminate as much bias as possible by replicating the experiment…

    Words: 777 - Pages: 4
  • Pathogenesis Of Endometriosis Summary

    endometrium of women who had endometriosis and those whom didn’t have it. The results of the two populations were compared. Due to this being done, it was shown that a particular protein known as DJ-1, is present and upregulated in eutopic endometrium of the woman surveyed with endometriosis. Therefore, it was inferred, that this protein may have impact or involvement of how endometriosis occurs. DJ-1 is a protein that is known to affect cell survival and growth as well as in ant oxidative…

    Words: 1260 - Pages: 6
  • Wet Mount Slide Essay

    The slide used during the Microscope Lab was a wet-mount slide which was used in order to view the specimens from the microscope. A wet-mount is a method for preparing specimens to view using a microscope. A myriad of specimens look better when placed in a drop of water on the microscope slide. The water supports the sample and fills the space between the coverslip and the slide. This allows light to pass easily through the slide, the sample, and the coverslip. Scientists use the wet-mount…

    Words: 557 - Pages: 3
  • Advantages And Disadvantage Of Electron Spectroscopy

    An electron vacancy is created and is further filled by an electron from the higher shell and thus an X-ray is emitted to balance the difference in energy between the two electrons. The detector placed on the energy dispersive spectroscope apparatus measures the number of emitted X-rays and their energies. Energy of the X-ray is characteristics of the element from which X-ray is emitted. A spectrum of the relative counts of the detected X-rays is obtained and evaluated for quantitative…

    Words: 1165 - Pages: 5
  • Electron Microscope

    Electron microscope (Manning, n.d.) is the instrument used to magnify different scientific artifacts with the used of electron beams to create the illustration of the sample, and has the capacity to magnify two million times of the original specimen. The development of the electron microscope was first known in the year 1931 because Ernst Ruska and Maximoll Knoll magnified electron image successfully, but the equipment was actually constructed in 1933 (Innes, n.d.). The concept of the electron…

    Words: 1104 - Pages: 5
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