Transmittance

Through calculation, the percent transmittance allowed for the determination of absorbance. To zero-out the spectrophotometer, about 2/3 of the blank solution was poured into a cuvette and placed in the spectrophotometer device until it displayed 100% (allowed for the measurement of the absorbance of just the phosphate). The solution in the cuvette was disposed in a designated beaker, then it was thoroughly rinsed with distilled water and dried with Kimwipes. About 2/3 of the first solution was poured into the cleaned cuvette and placed in the spectrophotometer for testing. The previous steps were repeated for each of the remaining solutions. Results The results obtained were calculated using the following formulas respectively. The initial volume of solution number 2 was determined…

wavelength is related to the energy of the light that is absorbed by a molecule. The color of the light reveals information about the kind of molecule that does the absorbing. The amount of absorption is dependent on the type of molecule.3 Each of the cuvettes filled with the prepared solution was measured in wavelengths that ranged from 350 nanometers to 650 nanometers in 50 nanometer percentage increases. As seen in Figure 1, there are no trends in the absorbance rates of each solution used;…

Percent transmittance is inverse to absorbance1. Percent transmittance will reach 100% if all light directed at a solution passes through; versus absorption which will be at zero if all light passes through. On the contrary, percent transmittance will reach zero if all light is absorbed. Thus, in relation to this experiment, a percent transmittance of 100% would indicate the enzyme has used up all of the substrate indicating a complete reaction. The time it takes for the enzyme to complete the…

Introduction: The goal of this lab was for students to learn how to use a spectrophotometer to find the absorbance of four known copper (II) sulfate solutions, four copper (II) sulfate solutions that were created by dilution, and one unknown copper (II) sulfate solution. The purpose of this experiment was to help students learn how to use the Spec-20, which is a machine that measures the transmittance and absorbance of a solution, learn about the linear relationship between absorbance and…

reduced clear state. We had three tubes with varying concentrations of succinate and measured the transmittance of each over a half hour period to determine whether more succinate led to more DPIP being reduced. The results showed that the tube with no succinate (no reaction)…

its color led to the formulation of this experiment. The experiment utilizes chloroplasts of spinach leaves to test the different color lights’ effects on photosynthesis. Green light is said to be the least effective at driving photosynthesis on the electromagnetic spectrum due to being mostly reflected and transmitted instead of being absorbed. Conversely, red light and white light is more effective at driving photosynthesis (Reece, 2011). Thus, a hypothesis was created stating that the rate of…

Then, it was set up at 600 nm and at 0% transmittance. Next, four cuvettes tubes were obtained and the control group and each of them were label, so the identification is more easy. Then, Blank Tube was prepared by adding 4.6 ml of phosphate buffer, 0.3 ml of mitochondrial suspension, and 0.1 ml of succinate with the aid of pipette pump. The blank solution was then covered with parafilm and shook to mix the reactants. Next, the spectrophotometer was calibrated. For this, the Blank Tube was…

Graph 4 demonstrates the correlation between the transmittance and concentration levels of KMnO4, in how as the levels of concentration increase, the transmittance decreases. The experiment during week 2 was very similar to week 1 except we used potassium phosphate instead of potassium permanganate. Table 3 demonstrates the effect of wavelength on T% and absorbance. The same equation used for Table 2 was used in Table 3 in order, to calculate the absorbance. The highest absorbance level was…

5 shows that the difference of transmittance of orange light through translucent red cups or no cups is due to chance because the p-value of their t-test is over 0.05. No matter what, this information refutes the hypothesis. Even though they are not consistent, only two out of five pieces support the above statement. However, data from another group does not correspond with above findings. D Band’s histogram shows how in their experiments, red light had the higher percent of orange light…

into the Spec 20. Transmittance was then set to 100% to calibrate the machine. Next, five test tubes were prepared and labeled with one of the standard solutions of 0.05 M, 0.1 M, 0.2 M, and 0.5 M CuSO4 in them. The Spec 20 was set to 600 nm and % transmittance was measured of the standard solutions. Absorbance was calculated and recorded. Then, 2 g of solid CuSO4∙5H2O was measured out and placed in a 100 mL beaker. Water was added (about 15 mL) to make a 20 mL of 0.5 M solution from CuSO4∙5H2O…