Lac operon

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  • Lac Operons

    Genetic mutations are the source of numerous hereditary diseases. This has been discovered by the comparison of two DNA sequences with bioformatic software. Two sequences are aligned and compared in order to locate mutations in the DNA (Module 4 Protocol,1). Escherichia coli aka E. coli was used as the test subject in this lab because it employs lac operons while transcribing mRNA. An operon is a unit of linked genes that regulates genes in charge of protein synthesis. A lac operon requires the presence of lactose to function. E. coli will only transcribe mRNA for specific enzymes when a certain sugar is detected in the surrounding environment. Lac operons are made up of a CAP binding site, -35/-10 sites, operator region, the start site…

    Words: 1587 - Pages: 6
  • Plasmid Synthesis

    Coli is used very commonly by other researchers and companies. Pharmaceutical companies use it to go drugs like insulin in large quantities to sell to the public. Researchers use it to invent most of the drugs that the pharmacy companies are using but also to see how other traits would act in different bacteria. Another procedure that could be done in the same manner as this one could be the LUX operon, which is the transcriptions of genes to create bioluminescent products (Swarztman, 1990).…

    Words: 1599 - Pages: 6
  • Two Things I Didn T Know Reflection

    Reflection Two Things I Didn’t Know The first thing I learned about the social work practice while at Mille Lacs County is that not all situations will play out how you want them to. Of course I knew that in a general sense; however, there were many situations that I was a part of and/or heard about at the county where things didn’t go down how they were expected to, wanted to, or maybe even should have. This job can be unpredictable with clients as well as with judges and that is something that…

    Words: 890 - Pages: 4
  • Summary: The Importance Of The Evaluation Process

    Lastly, working with communities is similar, again, to working with individuals, families, and groups; however, it differs in the sense that the worker will focus on an entire community and how that group was involved in their work versus the smaller units that are above (Berkenmaier et al, 2104). II. Quantitative Evaluation Mille Lacs County utilizes the quantitative evaluation process very well. The quantitative evaluation process is one in which requires a social worker and client to meet…

    Words: 1630 - Pages: 7
  • Flashback Techniques In Interview With The Vampire By Anne Rice

    "Interview with the Vampire" is one of the best-seller novels by Anne Rice that published in 1976. It is a thrilling novel, which depicts immortal vampires as the central characters. Anne Rice uses the flashback technique in the novel through which novel’s protagonist, the Vampire named Louis tells the story of his past life to a mortal journalist named Daniel during an interview. Louis wants to make the whole world aware of the existence of evil vampires. The story begins in San Francisco where…

    Words: 782 - Pages: 4
  • Gene Regulation Research Paper

    Bacterial genes regularly found in operons. Genes in an operon were transcribing as a group. They have a particular promoter. One another operon consist regulatory sequence of DNA. This can act as binding site for regulatory proteins. They stimulate or inhibit the transcription. Regulatory proteins often bind to the minor molecules. Which able to make the protein active/ inactive. By varying the capability to bind DNA. Some operons are inducible. That they be able to turn on by the presence of…

    Words: 1325 - Pages: 6
  • Proinsulin And Insulin Case Study

    1. What are proinsulin and insulin? How different is proinsulin from insulin? A: Insulin is a polypeptide hormone, protein, that is produced by the beta-cells of the pancreas. Proinsulin is a single peptide chain that is a precursor to insulin. It is produced by the beta-cells of the pancreas as well. The structure of proinsulin differs from insulin in that it contains a C-chain that forms a single peptide chain with other chains A and B. Insulin does not contain the C-chain and the A and B…

    Words: 598 - Pages: 3
  • Sexduction Lab

    Illustration of sexduction in E. coli using streptomycin resistant F-lac- recipients and F′lac+ donors Abstract The process in which DNA from a donor cell is transferred through physical contact into a recipient cell is known as conjugation. In the experiment, sexduction, a cross between F′ and F- plasmids, was demonstrated by mixing the donor and recipient, diluting them and using selective plating methods. The plasmids from the donor, recipient and transconjugant were isolated and analyzed…

    Words: 1708 - Pages: 7
  • Scientific Article Analysis Questionnaire

    research utilized synovial fluid (SF) harvested from uninfected patients undergoing knee surgery, as well as serum, and trypticase soy broth (TSB) as growth media for S. aureus. The strain selected for study is known as S. aureus LAC pulsed-field type USA300, a type of community acquired methicillin resistant S. aureus (MRSA). The organism and genetically altered variants will be used to infect each of the different fluids and tested for the appearance of agglomerates. C. What are the general…

    Words: 1572 - Pages: 7
  • Difference Between Bacteria And Eukaryotes

    meaning one gene equals one protein. Bacteria’s life history is a short lifespan, reproduces quickly in large number of individuals. Griffith finds that bacterial cells can be transformed. (Raven) There are two forms of bacteria, one that causes pneumonia and a nonvirulent that does not. The nonvirulent lacks enzymes to manufacture the coat of polysaccharide. Griffith performed a series of experiments with these types of bacteria on mice. Some mice died and some lived. In the end polysaccharide…

    Words: 792 - Pages: 4
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