Gel electrophoresis

Before we get into the specifics about this disease, let us look at the history of sickle cell disease. One type of sickle cell disease is sickle cell anemia. Anemia means a few abnormal amounts of red blood cells. The name sickle cell anemia is first coined in 1910 by U.S. physician James Bryan Herrick. In one of his severe anemia cases, he found abnormal shaped red blood cells. He described them as sickle-shaped cells. By 1949, U.S. physical chemist Linus Pauling displayed that hemoglobin in…

analyzed in a process called agarose gel electrophoresis. Because DNA contains phosphate groups, which are negatively charged, applying an electrical current across the DNA will cause it to move towards the negative side of the gel. Since the restriction enzymes have separated the DNA into varying lengths, the rate at which the DNA fragments move through the gel will be dependent on their size. The smaller fragments will be able to more rapidly traverse the gel while the larger fragments will do…

Through attending a neuroscience program called Camp Neuro at the New York Medical College and taking AP psychology, I have been able to apply my knowledge in myriad classes. My understanding of the biopsychosocial approach to psychology helps me analyze texts in English and moral situations in ethics. The interdisciplinary seminar Modes of Being would serve as a way to continue this intellectual exploration that I am already pursuing in conjunction with my major in psychology. I find that…

control, 2) RT negative control, and 3) RNA negative control. The purpose of preparing 3 different tubes is to ensure that our product is not contaminated and also to confirm that we are getting the right bands, when we run our product through the gel. Part a: Preparation of RNA tubes: First, you have to ensure that all your reagents and tubes are on ice because a minor change in temperature can greatly impact the reagents and products. In the RNA+RT tube, add 1 l RNA, 1 l random primer, and 3…

Protein-protein interaction can be defined as the interactions between different protein molecules within the cellular organization leading to formation of large protein complex which is modulated by non-covalent interactions such as hydrophobic interaction, hydrogen bond, van der Waals interactions etc. Protein-protein interaction plays an important role in functioning of body’s significant mechanism such as replication, transcription, translation, signal transduction, cell cycle, etc. Thus,…

As an aspiring M.D. or M.D./PhD, it is important to experience the research side of medicine and observe first-hand how it translates into the medical field. By having this clinical/biomedical research experience, I believe that I will have a more holistic and comprehensive perspective of healthcare and medicine that will help me become an exceptional physician one day. As a Neuroscience major following a pre-med track, I have covered a wide range of courses such as chemistry, physics, biology,…

I received a 4 on both AP biology and chemistry which correlates to me being well qualified in both areas of study. In AP biology I learned important skills in microbiology such as gel electrophoresis and gene transformation which allowed me to work closely with DNA sequences and how they affect phenotypes. Being familiar with genetics and DNA sequences would allow me to learn quickly in the lab when dealing with the topics of gene regulation…

Group Molecular Biotechnology Fong Fuk Ka (140280041) Liu Ka Hin (140123508) A. Results and analyses 1. Show your gel photos (PCR and RFLP) with clear figure numbers, titles and legends. 1 2 3 4 5 6 7 8 Figure 1 PCR products of two partial segments of ABO locus in human DNA shown on agarose gel from group 6. Well 1 was blank, well 2 was 100bp ladder, well 3 was own sample (5/6), well 4 was own sample (7/8), well 5 was unknown N…

serotype of this genus that is based on DNA molecular typing methods.() Typing methods must be simple, rapid, with great repetition and distinction power; however, current methods such as multi locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) are not only time-consuming, but also low repeatability.()The spa typing is a typing method in Staphylococcus aureus, which is founded on DNA sequencing of short sequence…

The clinicopathologic records for each patient were published previously [17] (Table 1). The average age of the randomly selected group of 100 breast carcinoma cases with stage I (24%), stage II (39%), stage III (33%), and stage IV (2%) and two patients undetermined disease was about 51.5 years. Fifty-eight percent of the patients were premenopausal cases, with an average age of 37.5 years. The average age of postmenopausal patients was 61.2 years. Numerous publications have reported aberrant…