(CYP450) are both enzymes that are involved in phase I metabolism. They are classified as microsomal enzymes that require oxygen and NADPH. These enzymes are involved in the conversion of lipophilic compounds to more hydrophilic metabolites by adding molecular oxygen, which ensures rapid excretion. There are genetic variants with both classes of enzymes, which may contribute to interindividual variability in drug response. Some of the differences between properties of these two enzymes…
phosphorylase b stored in the cell, as phosphorylase kinase is initiated. Phosphorylation of glycogen phosphorylase b converts to phosphorylase a. This enzyme releases glucose-1-phosphate from glycogen polymers. After this the phosphate group is moved from the C-1 position to the C-6 position creating glucose-6-phosphate by the enzyme, phosphoglucomutase. The enzyme glucose-6-phosphatase removes the phosphate group from glucose-6-phosphate, leaving free glucose molecules. These glucose molecules…
This lab looked at the different effects of an enzyme with concentration, pH, and temperature compared to the rate at which the enzyme works. The results showed a quick decrease when the temperature and pH has passed its most favorable conditions and or levels, but the concentration remains at the same levels throughout except pH6 and 40 degrees celsius. Both the pH and temperature had a steady increase in rate once the favorable conditions were being reached or are reached, but had a certain…
effect on the enzyme catalase, taken from a cut and blended potato. The blended potato mixture was strained and put into a centrifuge, the liquid that was in the centrifuge contained the enzyme and remained on ice. The enzyme was placed in test tubes that had varying levels of pH buffers, substrate concentrations and temperatures; the pH, substrate concentration and temperature were the three variables. The data showed that each variable contained an optimum level at which the enzyme was most…
Enzymes Two major factors influence how effective an enzyme is by promoting the rate of reaction. Each enzyme has a specific temperature that allows it to perform the most work. If an enzyme is placed in an environment under its optimal functioning temperature, the speed of the reaction will slow. Reason being, the molecules of the reactants reduce in energy from the low temperature, less movement means less reactions with the enzymes. At higher temperatures, molecules within an enzyme increase…
special proteins called enzymes. Enzymes are long chains of amino acids that have taken a very specific three-dimensional shape called a globular shape. This specific shape contains an area that is designed to match a specific molecule. The substance that an enzyme operates on is known as its substrate. Almost all metabolic reactions in organisms are catalyzed by enzymes. Many of these reactions occur in specific sequences and are called metabolic or biochemical pathways. Enzymes can be…
interacts with the hydrogen peroxide. The hydrogen peroxide is altered when two substances are combined. The oxygen gas, as a product, comes from the hydrogen peroxide. Enzymes are big proteins that speed up the rate of a chemical reaction by moving as a catalyst. A catalyst provides the environment for the reaction to occur. Catalase is an enzyme in the liver that breaks down dangerous hydrogen peroxide into water and oxygen. When this reaction takes place, oxygen gas bubbles breakout and…
An enzyme is a biological catalyst; catalysts are substances that increase the rate of chemical reactions without being used up. Enzymes also make the reaction take place more often, and are needed in small amounts; they are also neither reactants nor products. Enzymes are also proteins that are folded into complex shapes that allow molecules to fit into them. The enzymes that are involved in respiration, photosynthesis and protein synthesis work within cells whereas other enzymes are secreted…
Enzymes are organic catalysts that speed up reactions that occur by lowering the activation energy of the reactants. By lowering the activation energy, the chemical bonds are weakened, thus the reactions are much faster than without the catalyst. Enzymes are composed of proteins which shape dictates the function of the enzyme. On the enzyme it contains a part called the active site where the substrate binds and the chemical reaction undergoes. A substrate is the chemical involved in the…
experiment looks at the effects of pH, enzyme concentration, and temperature of the catalytic rate of different enzymes including alkaline phosphatase (ALP). Some of the enzymes used are involved in human digestion while others are involved in clinical tests for certain diseases (Wilson, D’Alessio, Schmitt Lavin, Keith, & BIOL 1500 Team, 2015). The ALP enzyme is used to diagnose diseases such as bone cancer, jaundice and cirrhosis of the liver. This enzyme also catalyzes the hydrolysis of a…