DNA polymerase

separating strands of DNA, is used to visualize and isolate strands of nucleic acids based primarily on length. The technique can be used on a mixed assortment of DNA molecules to yield information concerning the length…

genotypic differences that can be observed using DNA sequencing data. One way to observe genetic variation amongst humans would be to sequence the Mitochondrial DNA(mtDNA). Mitochondrial DNA is the most commonly used DNA to form phylogenetic trees. Human Mitochondrial DNA is about 16,500 bps in size and consists of 13 protein coding genes, 22 tRNAs (transfer RNA), and 2 rRNAs (ribosomal RNA) (Ingman, 2001). Mitochondrial DNA has many beneficial uses for DNA sequencing such as; its ability to be…

biology has become one which many are deciding to take on. We know that proper replication of the telomeric DNA at chromosome ends is critical for…

scientists’ stances on creating a perfect product is the research being done on recombinant DNA. Recombinant DNA is any single molecule containing DNA sequences from two or more organisms. The process of creating recombinant DNA relies on the use of restriction enzymes, gel electrophoresis, and DNA ligase. The first step in creating this new DNA strand is to cut the gene sequences you want from the original DNA molecule. This sequence will be cut by the restriction enzyme in such a way that it…

along with the prodigious rate it is still growing, have made them , while not necessarily obsolete, insufficient for the task. This is why the most promising method of storage in the current time is none other than the building blocks of life itself: DNA. But to understand how deoxyribonucleic acid as a site of information storage came to be, the progress of its predecessors must be understood. The first form of large scale information storage is one we are still using in the modern day,…

Maple Syrup Urine Disease Hannah Gentry , 13SK , 8/21/2014 Maple syrup urine disease (MSUD) is a rare genetic disorder where an aminoacidopathy secondary to an enzyme defect in the catabolic pathway of the branched-chain alpha-keto acid dehydrogenase complex, which are required in order to metabolize certain amino acids in the human body. In other words, it is a metabolism disorder, where the infant is unable to break down the amino acids: leucine, isoleucine, and valine. Build up of these…

prokaryotes, and therefore, must condense their DNA into chromatin. Chromatin is composed of histone proteins that help to condense and organize the DNA forming chromosomes. The basic unit of this chromatin is a nucleosome, which contains about 150 base pairs of DNA that are wrapped 1.7 times around the core histone proteins. However, this tightly wrapped chromatin becomes a problem when regulatory proteins need access to specific sections of the DNA. The genes are “turned off” to…

Cellular Metabolism: Deoxyribonucleic Acid (DNA) is replicated when enzymes begin to unzip the DNA. The enzymes do this by breaking the hydrogen bonds that are between the pairs. After this occurs, the unpaired bases are now free to bind with other nucleotides. They may only bind if the nucleotides have the appropriate complementary bases. DNA polymerase now attaches DNA nucleotides to one end of the growing strand of nucleotides. Now there are two DNA molecules, each has an original nucleotide…

Genetics Testing Introduction to Genetic Testing Genetic testing, also known as DNA testing, is the process of using medical tests used to look at one’s genes, inherited from one’s parents. These tests ranges from identifying a child’s biological parents, to ascertaining if there are any risks of having cancer or diseases. Results of genetic testing may help access on how severe a disease might be and find the best solution to diagnose it. It will also help determine the chances of developing…

characteristic supporting by the IPTG, which helps to release the RNA polymerase (T7). The results also shows that the transformation efficiency rate of the class…