The main objective of the experiment is to study and localize the fluorescent tags on specific chimeric proteins in a particular cell. The localization of these tags will generate a detailed understanding of the cytoskeleton in the cell, and the main function of using the Enhanced Yellow Fluorescent Protein. With the help of EYFP, the location of the proteins, and the molecular masses of the chimeric proteins should be really easy (Cannons, A. 2006). Consequently to better understand this experiment, it is crucial that certain terms are elaborated and define. The cytoskeleton is an elaborate network of rod running though the cytosol. It acts as a cell’s bones, muscles, and ligaments by supporting cellular structures and providing the machinery to generate various cell movements (Hopkins, 2010). There are three types of rods in the cytoskeleton, and each filament has specific role microfilaments Actin filaments are considered to be the most abundant in the cell; they also create contractile forces (Hopkins, 2010). Secondly, the Microtubules help with the duplication and the division of in the cells. Lastly, Intermediate filaments are use for the strength and the motility in the cell.…
Introduction Some people believe that we have only one type of muscle in our bodies that help us with all different types of tasks, however; not only do our bodies have different muscle types, they also contain different muscle fibers that are used for different activities. Skeletal muscle contain three types of muscle fibers: slow twitch, fast twitch oxidative, and fast twitch glycolytic. Most muscles in our bodies have different combinations of these muscle fibers, although our body recruits…
due to a gene mutation on the X chromosome, thus causing males to be more at risk. The gene mutation prevents the translation of the protein dystrophin (dys-tro-phin). Dystrophin is a cytoplasmic protein essential to the formation of protein complex connecting the cytoskeleton of a muscle fiber to the surrounding extracellular matrix via the cell membrane. The word cytoskeleton means skeleton of the cell. Cytoskeletons extend from the nucleus of the cell to plasma membrane consisting of a…
Microtubules are components of the cytoskeleton of the cell that are made up of tubulin. They are the largest component of the cytoskeleton which is a reason why much of cytoskeleton research centers focus on this specific element. In this paper entitled Microtubules Negatively Regulate Insulin Secretion in Pancreatic β Cells, understanding the role these microtubules play is key in understanding the research findings. Microtubules are made up of protofilaments that are composed of a dimer:…
tails and the hydrophilic regions interacting with the hydrophobic heads of the phospholipid bilayer. Note that is is due to the amphipathic nature of the bilayer and proteins. The mosaic of proteins are integral proteins and peripheral proteins. They carry out many functions: transport, enzymatic activity, signal transduction, cell-cell recognition, intercellular joining, and attachment to the cytoskeleton and extracellular matrix. 3. Diagram the cell membrane. Label the following parts: lipid…
they did not know what they might be in danger of. (“Silicosis”, 2017) Other than being able to cause cancer and silicosis, Arsenic is a neurotoxin, which is a chemical that disrupts the nervous system. Furthermore, the sublethal effect of Arsenic is classified as causing a cytoskeleton influence which means that it disrupts and destabilizes cytoskeletal framework. (Singh, 2011) Cytoskeleton is a structure that helps cell keep their shape and internal organization. It is necessary for processes…
It means that the proteins and other components that make up of one half of the lipid bilayer are different from the other half of the membrane. It is important because one of the half of the membrane has and carries different functions from the other half of the membrane. For example, one half of the external lipid bilayer is attached with glycolipid and carbohydrates and the other half is attached with cytoskeleton that binds on the internal membrane. Glycolipid and carbohydrates are important…
stacks called grana, which are the chloroplasts solar power packs. Cilia and flagella are made up of microtubules that are enclosed in an extension of a plasma membrane; cilia are short structures while flagella are long structures. A cell usually consists of one flagellum or two flagella, but a large number of cilia are present in a cell. They provide movement for organisms through water Next are the non-membranous organelles and the first will be the cytoskeleton, which are a series of…
reticulum, Golgi Apparatus and cytoskeleton. The nuclear envelope, which is a bilayer membrane that encloses the contents of the nucleus during most of the cell lifecycle as previously stated when discussing the basics of the cell. We revisit the nuclear envelope because it is also within the membrane. Lysosomes are membrane-enclosed organelles that contain an array of enzymes that are able to break down biological polymers-proteins, nucleic acids, carbohydrates, and lipids (The Cell: A…
Although the function of ICAM5 in the pituitary is unknown, its roles in the brain as a cell adhesion molecule have been extensively studied. ICAM5 is abundantly expressed in the dendritic filopodia and plays an important role in synapse formation with presynaptic axons, via the extracellular matrix molecule, vitronectin (63), and/or β1 integrins (32, 64). Once these connections are established, the extracellular domain of ICAM5 is cleaved, disrupting the cytoplasmic actin cytoskeleton through…