The main objective of the experiment is to study and localize the fluorescent tags on specific chimeric proteins in a particular cell. The localization of these tags will generate a detailed understanding of the cytoskeleton in the cell, and the main function of using the Enhanced Yellow Fluorescent Protein. With the help of EYFP, the location of the proteins, and the molecular masses of the chimeric proteins should be really easy (Cannons, A. 2006).
Consequently to better understand this experiment, it is crucial that certain terms are elaborated and define. The cytoskeleton is an elaborate network of rod running though the cytosol. It acts as a cell’s bones, muscles, and ligaments by supporting cellular structures and providing the machinery to generate various cell movements (Hopkins, 2010). There are three types of rods in the cytoskeleton, and each filament has specific role microfilaments Actin filaments are considered to be the most abundant in the cell; they also create contractile forces (Hopkins, 2010). Secondly, the Microtubules help with the duplication and the division of in the cells. Lastly, Intermediate filaments are use for the strength and the motility in the cell. …show more content…
The nitrocellulose is rinsed in the water, and then the clamp was placed in the Tupperware container filled with running buffer with the Scotchbrite pad. Two sheets of blotting paper was placed on the Scotchbrite pad then the gel was placed on the blotting paper with the nitrocellulose paper the top of it. Two more pieces of blotting paper were then added on top of all the sandwich followed by another Scotchbrite pad. Finally, the group closed the clamp on the apparatus then submerged the blotting sandwich in the running buffer where it’s then transferred to a gel apparatus, and run for about 45
Consequently to better understand this experiment, it is crucial that certain terms are elaborated and define. The cytoskeleton is an elaborate network of rod running though the cytosol. It acts as a cell’s bones, muscles, and ligaments by supporting cellular structures and providing the machinery to generate various cell movements (Hopkins, 2010). There are three types of rods in the cytoskeleton, and each filament has specific role microfilaments Actin filaments are considered to be the most abundant in the cell; they also create contractile forces (Hopkins, 2010). Secondly, the Microtubules help with the duplication and the division of in the cells. Lastly, Intermediate filaments are use for the strength and the motility in the cell. …show more content…
The nitrocellulose is rinsed in the water, and then the clamp was placed in the Tupperware container filled with running buffer with the Scotchbrite pad. Two sheets of blotting paper was placed on the Scotchbrite pad then the gel was placed on the blotting paper with the nitrocellulose paper the top of it. Two more pieces of blotting paper were then added on top of all the sandwich followed by another Scotchbrite pad. Finally, the group closed the clamp on the apparatus then submerged the blotting sandwich in the running buffer where it’s then transferred to a gel apparatus, and run for about 45