Bacteriophage

Being an inhabitant of all environments, from aquatic to terrestrial, from soil to distilled water, from plants to humans, P. aeruginosa is the quintessence of microbial arms depot. It produces a wide range of secondary metabolites to protect its niche from other fungi and distantly related bacteria. In order to fight fellow Pseudomonads and other closely related bacterial species that may compete for common niches, all strains of P. aeruginosa also produce a broad range of bacteriocins referred…

Eukaryotes typically do not use rolling circle replication, its typically used by bacteriophages and viroids. Rolling circle occurs when a circle chromosome replicates only one strand of DNA (as mentioned above its usually the lagging strand). Some eukaryotes can have rolling circle transposons but in general Eukaryotes have linear DNA and…

In the 1920s, A British researchers named Frederick Griffith studied bacterial pneumonia to find a vaccine for it. The specific bacteria was Streptococcus pneumoniae. Using streaking on a cultured Petri dish, the batterie was seperated into two strains, or colonies. The R-Strain was rough, and when injector into mice, it did not cause pneumonia, but the S-strain was smooth and slimy, and caused lethal pneumonia when injected. When the S-Strain was killed by heat, it did not cause sickness, but…

Advances in biology have led to many life altering medications, treatments, and solutions to molecular issues. One such region of biology that has altered scientists’ stances on creating a perfect product is the research being done on recombinant DNA. Recombinant DNA is any single molecule containing DNA sequences from two or more organisms. The process of creating recombinant DNA relies on the use of restriction enzymes, gel electrophoresis, and DNA ligase. The first step in creating this new…

Many of you would’ve heard the word Antibiotic at least once in your life, a few of you may have even taken some. It’s a very well known medical term and literally means ‘life-killing’. Something I’m certain will astound you, is that growing resistance against these once revolutionary treatments is one of the biggest threats to global health today. For those who don’t know, Antibiotics are a branch of drugs that stop bacteria from reproducing. This is achieved by stopping vital processes…

) Discuss antibiotic resistant bacteria, cause and effect. Antibiotic resistant bacteria is a big concern now a day due to the use of excessive and improper use of antibiotics. It is very common for people to star taking an antibiotic and stop the regimen as soon as the symptoms are gone. With this practice, bacteria that were not killed by the antibiotic but were exposed, are capable of becoming immune to it. This immunity or resistance can be passed on from bacteria to bacteria through the…

antibiotic resistant microbes had led researchers to consider taking a closer look at bacteriophage therapy as an alternative to treating infections with broad ranging antibiotics. Phage therapy was developed in the Soviet Union decades ago as way of treating bacterial infections because the trade embargo with the US prevented the soviets from acquiring adequate amounts of antibiotics (Reardon, 2004). Bacteriophages are viruses that specifically attack bacteria to treat infections by…

Literature Review: Green Fluorescent Protein and Histidine Tagged Proteins Histidine and green fluorescent protein (GFP) tagging are important in molecular biology because they allow for purification, tracking, and quantification of target proteins (Ferrari et al., 2004; Cho et al., 2011; Deponte, 2012). Histidine tagging provides a method for isolating and increasing the amount of target protein recovered from a biological organism or mixed sample of proteins (Masek et al., 2011; Singh and…

coli. (Ishino et al., 1987) CRISPR are sections of prokaryote DNA with short repeating sections of base sequences, interspaced by foreign Spacer DNA from exposure to a bacteriophage virus. (Marraffini and Sontheimer, 2010) CRISPR is a natural process that occurs in bacteria as a defence mechanism. Bacteriophages hunt and kill bacteria by inserting their genetic code into the bacteria. This kills the majority of bacteria, however, any survivors capture the Viral DNA which confers an immunity…

She was best known for discovering a virus that infected bacteria. She called this virus lambda bacteriophage and discovered it in 1951 while she studied at the University of Wisconsin. She and her husband, Joshua Lederberg produced a way that they could easily transfer bacteria from one petri dish to another. They called his process replica plating. In…