Agarose gel electrophoresis

the victim’s shirt. These evidences were taken to examination. The objective of this crime scene is to examine a forensics question using techniques to extract and analyze the DNA and to separate the DNA fragments of different sizes by agarose gel electrophoresis. Our Hypothesis was that suspect 2 committed the crime, our prediction was that if suspect 2 committed the crime then DNA from the crime scene would have matched the suspect’s…

Introduction: In this lab experimentation, the group conducted Deoxyribonucleic acid isolation and restriction analysis on a plasmid from Escherichia coli cells. Plasmids are small circular DNA that are in the bacterium cells. Escherichia coli is a gram- negative bacterium that is known for variable reaction to antibiotics, and can be genetically manipulated. The gram- negative bacterium, Escherichia coli can be genetically manipulated by extracting a certain plasmid that allows it to resist…

region in the synechocystis that gets amplified is between 662 to 2,819 bp which makes the target gene approximately 2,157 bp. We used primers to flank the psbC gene and primers to identify the presence of KanR gene. When the sample was loaded onto the gel, it separates the fragments by size, by using a marker at the 2,100 and comparing it against the samples one can see that the psbC gene is the predicted size. For the psbC gene to be visible, a loading dye was added to the sample, improper…

creating a perfect product is the research being done on recombinant DNA. Recombinant DNA is any single molecule containing DNA sequences from two or more organisms. The process of creating recombinant DNA relies on the use of restriction enzymes, gel electrophoresis, and DNA ligase. The first step in creating this new DNA strand is to cut the gene sequences you want from the original DNA molecule. This sequence will be cut by the restriction enzyme in such a way that it creates a palindrome…

century. The appreciation that individual genetic variation could be used to exclude suspected individuals from criminal activity propelled the analysis of other gene products including isozyme and polymorphic protein determination by slab gel electrophoresis. Forensic DNA analysis was introduced in the 1980s and became established with the discovery of polymorphic mini-satellites or variable number of tandem repeat (VNTR) loci which gave rise to the concept of genetic fingerprinting in forensic…

Group Molecular Biotechnology Fong Fuk Ka (140280041) Liu Ka Hin (140123508) A. Results and analyses 1. Show your gel photos (PCR and RFLP) with clear figure numbers, titles and legends. 1 2 3 4 5 6 7 8 Figure 1 PCR products of two partial segments of ABO locus in human DNA shown on agarose gel from group 6. Well 1 was blank, well 2 was 100bp ladder, well 3 was own sample (5/6), well 4 was own sample (7/8), well 5 was unknown N…

The clinicopathologic records for each patient were published previously [17] (Table 1). The average age of the randomly selected group of 100 breast carcinoma cases with stage I (24%), stage II (39%), stage III (33%), and stage IV (2%) and two patients undetermined disease was about 51.5 years. Fifty-eight percent of the patients were premenopausal cases, with an average age of 37.5 years. The average age of postmenopausal patients was 61.2 years. Numerous publications have reported aberrant…

Report begins: The point of existence for a species is to survive and reproduce. The human race is a thriving species with a population that is forever increasing. One of the biggest problems for the human race is curing or preventing diseases that reduce the quality of life and even kill the individuals affected by it. An example of a disease is Adenosine Deaminase deficiency (ADA) which is a very rare disease that creates a poor immunity in the individual affected due the the lack of the…

Inflammation is a defense mechanism where the body’s white blood cells fight off bacteria, viruses, and damaged cells(Feeling the Heat- the Link between Inflammation and Cancer, 2013) . When pathogenic cells enter the body, the white blood cells surround that invasion site immediately(Feeling the Heat- the Link between Inflammation and Cancer, 2013) . The white blood cells then release a specific toxic chemical that denatures the pathogen enough to kill it(Feeling the Heat- the Link between…

Human Manipulations of Genetic Transfer and its Biological Implications For years, humans have been able to manipulate the alleles in animals for favourable traits through the use of selective breeding. But, now that technology has become so advanced, humans have developed a new process of manipulating the gene transfer through transgenesis, where scientists can create new alleles in animals. This essay shows how the selective breeding method and transgenesis method are carried out on the…