Agarose gel electrophoresis

The science of my topic is revolves around criminal law and forensic science. According to staffs. ac. uk “Forensic science is any science used for the purposes of law, and therefore provides important scientific evidence for use in the courts of law, e.g. in a criminal investigation and trial.” Today's world got a lot of new technology and that makes it easy for forensic scientists to solve more crimes than ever before. Also the field has grown exponentially over the years. Forensic science…

recipient, diluting them and using selective plating methods. The plasmids from the donor, recipient and transconjugant were isolated and analyzed using FIGE gel Electrophoresis. The data that was acquired consisted of 12 red colonies and 483 white colonies from the conjugation mixture with a conjugation efficiency of 2.42%. The FIGE gel Electrophoresis produced the DNA bands of the donor and transconjugant strains with a size of 112 kb and no bands for the recipient. The results show that the…

David Daige Biol 1030H Due Date: April 1, 2016 Professor Laura Pacey Lab Report 5: Molecular Biology Techniques Abstarct The aim of this study is to accurately measure small volumes using the micropipettes and to use virtual STR and PCR programs to test the paternity of various cases. During the lab, 18uL of different volumes were successfully measured into a tubule using the micropipette. During the lab, fruit juice was accurately extracted from a solution using…

Certain single nucleotide polymorphisms in the human lactase phlorizin-hydrolase gene have been associated with the ability of certain individuals to constantly digest milk products. These mutations in the lactase gene lead to constitutive expression of lactase, which is then used to break disaccharide lactose into glucose and galactose constantly throughout adulthood as lactase persistence. In particular, past research has shown that some individuals of the African and European descent have…

and Methods: Blood was drawn and DNA extracted from 90 subjects (46 cases and 44 controls). The 4G/5G polymorphism of PAI-1 was amplified using specific primers. Amplified products were visualized by staining with Ethidium bromide after electrophoresis in agarose. Risk factors PAI-1, MDA, hs-CRP and lipid profile were compared between 46 angiographically diagnosed coronary artery disease(CAD) patients and 44 age/sex matched healthy subjects. Furthermore,…

This hospital-based case-control study was conducted on 110 consecutively newly diagnosed LP patients and 120 apparently healthy, age and sex-matched, control subjects. The control group consisted of participants referred for non-inflammatory dermatological conditions such as chronic hair loss or coming with their relatives. They had no oral mucosal, systemic or autoimmune diseases, and had no history of LP. All subjects were recruited from the outpatient clinic of the Department of Dermatology,…

Introduction Charles Darwin is famous for his book, The Origin of Species, where he proposed that evolutionary change in populations is due to natural selection. His idea was that of survival of the fittest. In other words, the species with superior traits would have more of a likelihood to live, and then breed more offspring. Slowly, more and more of the species with the superior traits will survive and multiply. But what is it that gives these species the better survival trait, and allows…

A mass quantity and variation of organisms live in different regions around the world. Each organism is classified into groups depending on the characteristics of the species, this is called taxonomy. Invented by the Swedish scientist, Carolus Linnaes, the taxonomy system simplifies organism classification by ordering the species into seven major categories: Kingdom, Phylum , Class , Order , Family , Genus , and Species (Meier, Shiyang, Vaidya, & Ng, 2006). However, what if the species is…

DNA fingerprinting was discovered on September 10 1984 by Alec Jeffreys. His team at the university of Leicester had been working for seven years to see if it would be possible to tell people apart and whether they were related by using their DNA. They had been looking at regions of the human genome called VNTRs which stands for variable number tandem repeats. These can be thought of as a repeating string of genetic letters or basis, a bit like the same word written down many times along a line…

Introduction: Lactase is an enzyme that helps to digest lactose, a sugar found in many dairy products (U.S National Library of Medicine 2013). This enzyme is made through instructions provided by the LCT gene. Lactase is primarily produced and found in the cells that line the walls of the small intestine. At the brush border, an area where microvilli absorb nutrients from food as it passes by, lactose breaks down lactose into simpler sugars like glucose through a hydrolysis reaction (Biology…