Agarose gel electrophoresis

and there is no migration in or out of the population. In this experiment, the Hardy Weinberg equation will be applied to the class Alu genotype results to determine if the class population is in equilibrium. Cheek cell swabbing, PCR, and gel electrophoresis will all be done in order to find out if the student is homozygous dominant, heterozygous, or homozygous recessive for the Alu insert. The results from all of the individual tests done in the class will not be indicative of Hardy-Weinberg…

10.3, gel electrophoresis. The relevance of this study is important to field of biology because bacterial infections could be treated with bacteriophages. Bacteriophages can be a solution to antibiotic resistance because phages are viruses that are able to infect bacteria. This can tremendously help and set a new pathway in biology. The results were that phage was isolated. The conclusion is that gordonia host didn’t produce results, which…

Purification and Quantitation of DNA and Plasmid Structure Labs 3 and 4 Biology 453 Clayton Pedigo September 14 and 21, 2016 Introduction: In this report, there are two labs being discussed. The first lab being lab 3 (purification and quantitation of plasmid DNA) involved lysing bacterial E.coli cells and retrieving the plasmid particles. The purpose of the second lab, lab 4 (plasmid DNA structure), was to use the concentrated DNA from lab 3 and compare…

should have bacterial lawn as results showed. With better and more accurate results, the transformation efficiencies would have changed as well with higher numbers in ug units. Miniprep To Purify And Isolate plasmid DNA-  Figure 3: Miniprep Gel Electrophoresis. Figure 3 Key: Components of each well. The photo shows the results of the Miniprep, which allows for the identification of the unknown sample. Well #3 (the unknown) has the highest band above the 10kb line, another band a…

thermal cycler and the desired target sequence of DNA was amplified. In the final week of lab, the DNA was analyzed using an electrophoresis reaction in Agarose gel. This was done according the the instructions outlined in the Practicing Biology for Biology 151 lab manual. Finally the gel was placed under a source of UV light which allowed the DNA sequences to appear on the gel. A photograph was then taken to be further…

A process that can turn a single copy of a gene into more than a billion copies in just house is calles the polymerase chain reaction(PCR). PCR allows medical researchers to make many copies of a gene whenever they want to genetically engineer something. The DNA structure for years has been relatively challenging to study. Ultimately DNA is very long and tiny. Luckily there has been many advancements in DNA technology that have made working with DNA much easier. Especially in the tools and…

Lynchel Brumaire DNA, the genetic code of all organisms, can help in the analysis of the organisms evolutionary history. All organisms, alive and extinct, descended from one creature. As time went on mutations accumulated in the DNA sequence of the progeny. By sequencing the genetic code of an organism in relation to another, their evolutionary history can be placed onto a phylogenetic tree. In this experiment, DNA barcoding was used to to identify a species and place it onto a phylogenetic tree…

Introduction Plasmids, circular pieces of DNA that can replicate independently from the host cell’s DNA, have been extensively used to transform cells in biological research to study the effects of particular genes of interest (Lau et al., 2013). A plasmid’s basic components include a replication origin, a DNA marker, and a multiple cloning site, which are sufficient for the plasmid to replicate itself and help the transformed cell to exhibit characteristics indicating successful transformation…

obtained from Alfa Aesar, Heysham, UK. Cisplatin, Bradford reagent, trichloroacetic acid (TCA), thiobarbituric acid (TBA), reduced glutathione (GSH), glacial metaphosphoric acid (Gmpa), 5,5ˊ-dithiobis-(2-nitrobenzoic acid) (DTNB), proteinase K and agarose were purchased from Sigma-Aldrich Co, MO, USA. All other chemicals and solvents used were of the highest purity grade available. 2.2. Animals Sixty Adult male albino rats (180-200g) were obtained from the animal farm of the Egyptian Holding…

amplified PCR DNA was then run on 1% agarose gel to separate the amplified DNA. The gel was excised after the electrophoresis, and later subjected to gel purification to recollect the RNAIFAD2.1 and RNAIFAD2.2 DNA using the standard protocol provided with the GeneJet Gel Extraction Kit. The DNA after transcribed in the cells of the pennycress will adopt a miRNA shape due to its complementary sequence flanked by inverted repeats favoring a hairpin formation. The purified gel extracted RNAIFAD2.1…