Acetone

The purpose of a gram stain is to divide microorganisms into gram positive or gram negative. In this procedure you will use Crystal violent, Iodine, Acetone, and Safranin. You use different dyes because Gram negative microorganisms have a higher lipid content and it’s soluble in acetone whereas gram positive microorganisms are less permeable after acetone and the crystal violet-iodine becomes trap inside the cells. When preforming this test or any other test it’s important to use your…

plant was taken out of the soil, and all the excess soil was taken off. Some of the plant, 0.01g, to be exact, was cut from the plant. The plant then had its mass measured and it was recorded. Next, that 0.01g of leaf was ground up with 3mL of 85% acetone solution for five minutes. The mixture was filtered then supplemented to 3mL. It was put through a spectrophotometer and had its absorbance level read. The absorbance amounts at 663 nm and 644 nm were used in two equations to calculate the…

The amount of a milliliter of acetone per 50 mg crude material was added to the 25 mL Erlenmeyer flask. The flask was warmed on the sand bath to dissolve the acid (note: acetone has low BP). The solution was cooled to room temperature for about 5 minutes. If no crystals were formed, scratch the inside surface of flask with glass stirring rod to induce crystallization…

of a molecule’s hydrogen content.6 The following chemicals were used in the experiment: 9-fluorenol, sodium hypochlorite (the oxidizing agent), sodium thiosulfate (the reducing agent), sodium bicarbonate, acetic acid, and acetone.3,6 To begin the experiment 9-fluorenol, acetone, acetic acid, and sodium hypochlorite were added in sulfate is added to remove excess water from the hexane layer that is collected using the separatory funnel.6 To determine the success of the experiment, percent yield…

that simply showed that the unknown compound was a powdery, white solid with no smell. A qualitative solubility test was performed next. In this test, the substance was soluble in water, which proved it to be either polar or ionic, and insoluble in acetone, which proved the unknown substance to be an ionic compound. A quantitative solubility test was also performed, which showed that the solubility of…

bright orange, and the color change of the flame was recorded before rinsing it again with acid. To further identify the unknown compound, an additional solubility test was performed using acetone as the solvent. After weighing another 250 mL beaker and 0.1 grams of unknown solid, approximately 10.0 mL of acetone was added. The compound was found…

Nanotechnology can help diabetic patients monitor and control their blood glucose levels. I choose to write about this topic for the following reasons; I was recently diagnosed with the disease, and there is an alarming number of Americans suffering from this disease today. With the help of Nanotechnology, I believe we are on track to a cure in the near future. Here are few of the statistics. There are two types of diabetes diagnosis which are type 1 and type 2. Type 1 diabetes is…

The solid was then isolated through vacuum filtration, resulting in pale yellow-white powder. Next, acetone was used to dissolve the powered. This solution, a slightly cloudy yellow liquid, was then centrifuge, resulting in a clear yellow solution with solid impurities. The purified solution was then heated to remove the acetone. This step caused the product solution to become oily. Again, the solution was scratched, and 1.3331 g of powdery substance formed. Finally…

distinct smell, thus concluding that the compound is ionic. The solubility tests indicated that the compound was soluble in water, NaOH, and HCl and non-soluble in acetone and toluene as shown in Table 1. The compound was soluble in water indicating that the compound is either polar or ionic, but not soluble in toluene or acetone eliminating it being polar or nonpolar, making our compound ionic. The compound was also soluble in both NaOH and HCl, which are contradicting because a compound…

Extraction using Different Solvents The grounded Ixora coccinea and Beta vulgaris plant samples were extracted using the following solvents: Acetone, ethanol, methanol and ethyl acetate. The absorbance was noted to select the best choice of solvent for extraction. Inference Acetone, ethanol and methanol showed higher values. But, the dye extracted from acetone and ethanol did not fix to the fabric properly. So, methanol was selected as solvent for extraction of the natural dyes from the plant…