My gel had a few problems that made it unusable. The first and most important error was the lack of any bands in the unknown protein well. The lack of any bands in that well was most likely due to the improper filling of the well. When I was filling the well, I got several air bubbles, which would imply that the protein was not actually filling in its intended well. Nothing showed up in the well (not even a dye front). Obviously, this made it impossible to determine the molecular weight of the unknown protein. Another less serious error was the tearing of the gel when removing it from the gel holder. This made the gel harder to read, but it could still be salvaged. Overall, the gel being torn was not a disqualifying factor. Finally,…
DNA Gel Electrophoresis Electrophoresis is a process in which macromolecules are separated by utilizing their electrical charge and size. A technique for separating protein molecules of varying sizes in a mixture by moving them through a block of gel, as of agarose or polyacrylamide, by means of an electric field, with smaller molecules moving faster and therefore farther than larger ones. Gel electrophoresis of DNA is used in DNA profiling. When DNA is found at a crime scene, it's sent to a…
under such condition. Another reason could be that the cell did not get enough heat shock, or not enough pre-incubation time with CaCl2, which means the bacterial cell wall was not permeable enough for the DNA to come inside and interact with the bacterial DNA. The DNA gel electrophoresis showed that there were no fragments…
The technique used in this experiment was serum protein electrophoresis to determine the levels of proteins in cow serum. The objective of this experiment is to perform a gel electrophoresis of cow serum by agarose gel electrophoresis and to interpret the banding patterns produced by the migration of the serum. Electrophoresis is the shift of ions in an electric field in which the protein moves in the gel based on size as well as in electric charge. The electrophoretic mobility of small…
Jenna BSC 2010L-005 Using Gel Electrophoresis to Solve a Crime Materials and Methods: To begin this experiment, the DNA samples from suspects 1 and 2 needed to be digested with 2 different restriction enzymes. Four reaction tubes were required to complete this step. 10 uL of the reaction buffer was added to each reaction tube (1-4). Reaction tubes 1 and 2 were both filled with 15 uL of suspect 1’s DNA while tubes 3 and 4 were filled with 15 uL of suspect 2’s DNA. 15 uL of enzyme…
RESULTS Transformation: the transformation of the ade2 gene to the kanamycin resistant gene (ade2::kan2R) cause the cell to become red and grow on mediums containing G418 or kanamycin resistant mediums was observed to have occurred. PCR and Gel Electrophoresis: Figure 1 is the product of gel electrophoresis containing the wild type and transformed PCR products, either being or not being cut by HindIII. From the left (reader’s left) of the gel to the right the lanes are; 1.Ladder, 2. transformed…
Agarose gel electrophoresis of DNA is used to seperate mixtures of protein and nucleic acid fragments. Molecules tend to differ in size, charge, and mobility. The objective of this procedure is to predict the distance of DNA migrating towards the positive electrode in the electrophoresis chamber. Many scientists use agarose gel electrophoresis to examine the DNA of criminal suspects. The first step in this procedure is to prepare a buffer containing 1% of the liquid agarose gel. Once the gel…
Gel electrophoresis is used to separate DNA, RNA, and protein molecules by using an electric field. Protein gel electrophoresis is similar to agarose gel electrophoresis, but runs protein bands instead. The technique of protein gel electrophoresis uses cross-linked polymers of acrylamide (which is a neurotoxin and requires careful handling) or polyacrylamide to separate proteins and small nucleic acids. This technique takes longer than the agarose gels used to separate larger nucleic acids.…
To examine the DNA, a gel electrophoresis test needs to be run. The first step of gel electrophoresis is to set the gel, which is made of agarose. Agarose is used to separate DNA molecules, and acrylamide is used to separate proteins. The gel starts off as a liquid, which is poured into a molding tray and after it sets, it will turn into a solid. Next .all of the DNA needs to be collected and copied with a Polymerase Chain Reaction, PCR, which is vital to the success of the gel process because…
Agarose Gel Electrophoresis Since the discovery of the four classes of macromolecules (proteins, lipids, carbohydrates, and nucleic acids), researchers and biochemists have fine-tuned methods that reveal structural information about each of them. Different structural properties determine different functions and chemical properties, and scientists have developed intricate techniques to observe, quantify, and isolate molecules based on their aforementioned properties. Agarose gel electrophoresis,…