RESULTS
Transformation: the transformation of the ade2 gene to the kanamycin resistant gene (ade2::kan2R) cause the cell to become red and grow on mediums containing G418 or kanamycin resistant mediums was observed to have occurred.
PCR and Gel Electrophoresis: Figure 1 is the product of gel electrophoresis containing the wild type and transformed PCR products, either being or not being cut by HindIII. From the left (reader’s left) of the gel to the right the lanes are; 1.Ladder, 2. transformed PCR product, 3. transformed PCR product cut with HindIII, 4. wild type PCR product, and 5. wild type PCR product cut with HindIII.
Fragment Length: To determine the fragment length the ladder was used to determine standard curve. From there the …show more content…
The wild type phenotype id white, it will change to red when the yeast is unable to synthesis the ADE2 gene correctly, this causes a buildup of oxidative AIR in the vacuoles. Along with the phenotypic change the ade2::kanR gene will allow for the resistance of kanamycin which can detoxify the antibiotic G418. Then we selected only the transformed colonies by introducing the antibiotic G418 to the medium. The wild type will not be able to detoxify the G418 and they will …show more content…
Avery et al. (1944) made the observation that there was a transforming factor in DNA. This was confirmed by identifying that the genetic material and DNA have the same ratios of organic molecules. Our observations in this experiment showed that the wild type DNA then introduced to exogenous DNA transformed. These transformations lead to the phenotypic change and resistances to the antibiotic G418. The exogenous DNA was the only possible genetic material present. This experiment was made possible because budding yeast has a very short gene sequence and its entire genome has been crafted. Budding yeast is a model organism in the genetic world. This is due to the yeast ability to take up exogenous DNA both artificially and naturally (Mitrikeski 2013). Yeast is able to take in exogenous DNA and become biologically stabile at an outstanding rate. This high ability for yeast to transform is evolutionary important because it allows the yeast to have a high degree of biodiversity (Mitrikeski 2013). Other studies have also used budding yeast to test for antibiotics; prions are one of this study. Prions are misfolded proteins that can cause neurodegeneration; in a study they used budding yeast to identify compounds that can fight these proteins by transforming budding yeast into PSI+ and URE3 and testing them. The use of the cassette ADE2 was used to replace DAL5 gene to screen for the URE3 prion, like our
Transformation: the transformation of the ade2 gene to the kanamycin resistant gene (ade2::kan2R) cause the cell to become red and grow on mediums containing G418 or kanamycin resistant mediums was observed to have occurred.
PCR and Gel Electrophoresis: Figure 1 is the product of gel electrophoresis containing the wild type and transformed PCR products, either being or not being cut by HindIII. From the left (reader’s left) of the gel to the right the lanes are; 1.Ladder, 2. transformed PCR product, 3. transformed PCR product cut with HindIII, 4. wild type PCR product, and 5. wild type PCR product cut with HindIII.
Fragment Length: To determine the fragment length the ladder was used to determine standard curve. From there the …show more content…
The wild type phenotype id white, it will change to red when the yeast is unable to synthesis the ADE2 gene correctly, this causes a buildup of oxidative AIR in the vacuoles. Along with the phenotypic change the ade2::kanR gene will allow for the resistance of kanamycin which can detoxify the antibiotic G418. Then we selected only the transformed colonies by introducing the antibiotic G418 to the medium. The wild type will not be able to detoxify the G418 and they will …show more content…
Avery et al. (1944) made the observation that there was a transforming factor in DNA. This was confirmed by identifying that the genetic material and DNA have the same ratios of organic molecules. Our observations in this experiment showed that the wild type DNA then introduced to exogenous DNA transformed. These transformations lead to the phenotypic change and resistances to the antibiotic G418. The exogenous DNA was the only possible genetic material present. This experiment was made possible because budding yeast has a very short gene sequence and its entire genome has been crafted. Budding yeast is a model organism in the genetic world. This is due to the yeast ability to take up exogenous DNA both artificially and naturally (Mitrikeski 2013). Yeast is able to take in exogenous DNA and become biologically stabile at an outstanding rate. This high ability for yeast to transform is evolutionary important because it allows the yeast to have a high degree of biodiversity (Mitrikeski 2013). Other studies have also used budding yeast to test for antibiotics; prions are one of this study. Prions are misfolded proteins that can cause neurodegeneration; in a study they used budding yeast to identify compounds that can fight these proteins by transforming budding yeast into PSI+ and URE3 and testing them. The use of the cassette ADE2 was used to replace DAL5 gene to screen for the URE3 prion, like our