To examine the DNA, a gel electrophoresis test needs to be run. The first step of gel electrophoresis is to set the gel, which is made of agarose. Agarose is used to separate DNA molecules, and acrylamide is used to separate proteins. The gel starts off as a liquid, which is poured into a molding tray and after it sets, it will turn into a solid. Next .all of the DNA needs to be collected and copied with a Polymerase Chain Reaction, PCR, which is vital to the success of the gel process because if the DNA is not copied and someone accidentally messes up, there would be no way to run another test because there is not another copy of the DNA available. Next, the DNA is chopped with restriction enzymes, in this case, it was Hind III and Ptsl.
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The gel electrophoresis apparatus is then turned on and the DNA begins to migrate from the negative side to a positive, which is due to DNA being negatively charged. This process takes Restriction Fragment Length Polymorphisms (RFLPs) and migrates them. The longer the RFLP, the slower it moves and the farther it is up on the gel. The short the RFLP, the faster it travels and the lower it is on the gel. After the gel is ran, it needs to be analyzed to determine whose DNA it belongs to. Determining the DNA was based on examining the missing people’s DNA and comparing them with the controls and seeing whose DNA matched. After examining the DNA that was ran, it is clear to see that the DNA matches missing person number one because lane one had three bands, lane two had two bands, lane three had two, lane four had one, lane five had three, lane six had two, and lane seven had three. This clearly shows that Ptsl further proved that the DNA matched missing person number one because the segments matched up.
Further Analysis:
Facial reconstruction is a technique used in many forensic investigations in order to interpret the parts of a skeleton that …show more content…
Beginning with forensic odontology, it is a scientific study that applies dental records and compares the bones found. HealthCareers.og states that with the dental records, scientists are able to identify unknown human remains and bite marks (ExploreHealth, 2016). Odontology would apply to this case because the skull was found and examined. All of the teeth remained intact, so odonatologists would be able view dental records of known missing persons and identify if the teeth belonged to the missing persons that were found. Another analysis that could be used would be pathology. Forensic pathology is determining how the corpse died. According to the National Institute of Justice, a pathologist could recovery many things from a corpse (Forensic Pathology, 2013). Pathology would be a little difficult in this case due to the fact that there would be no flesh to determine if there were gunshot wounds or things of that nature. However, in this case there were bones, and pathology also includes taking bone marrow from the bones that were found. The bone marrow could be used to test the DNA of a missing person and compare it to the bones found at the scene. If the marrow matched, then that would identify the missing
The gel electrophoresis apparatus is then turned on and the DNA begins to migrate from the negative side to a positive, which is due to DNA being negatively charged. This process takes Restriction Fragment Length Polymorphisms (RFLPs) and migrates them. The longer the RFLP, the slower it moves and the farther it is up on the gel. The short the RFLP, the faster it travels and the lower it is on the gel. After the gel is ran, it needs to be analyzed to determine whose DNA it belongs to. Determining the DNA was based on examining the missing people’s DNA and comparing them with the controls and seeing whose DNA matched. After examining the DNA that was ran, it is clear to see that the DNA matches missing person number one because lane one had three bands, lane two had two bands, lane three had two, lane four had one, lane five had three, lane six had two, and lane seven had three. This clearly shows that Ptsl further proved that the DNA matched missing person number one because the segments matched up.
Further Analysis:
Facial reconstruction is a technique used in many forensic investigations in order to interpret the parts of a skeleton that …show more content…
Beginning with forensic odontology, it is a scientific study that applies dental records and compares the bones found. HealthCareers.og states that with the dental records, scientists are able to identify unknown human remains and bite marks (ExploreHealth, 2016). Odontology would apply to this case because the skull was found and examined. All of the teeth remained intact, so odonatologists would be able view dental records of known missing persons and identify if the teeth belonged to the missing persons that were found. Another analysis that could be used would be pathology. Forensic pathology is determining how the corpse died. According to the National Institute of Justice, a pathologist could recovery many things from a corpse (Forensic Pathology, 2013). Pathology would be a little difficult in this case due to the fact that there would be no flesh to determine if there were gunshot wounds or things of that nature. However, in this case there were bones, and pathology also includes taking bone marrow from the bones that were found. The bone marrow could be used to test the DNA of a missing person and compare it to the bones found at the scene. If the marrow matched, then that would identify the missing