Bio Lab- Denaturing Proteins
Period B- Ms. Dann
October 8, 2014
Nick D’Ambrosio, Matt Hermann, Carly Pruitt
Title: Electrophoresis and Protein Weight
Objective: The objective of this experiment was to gain a further understanding of gel electrophoresis, and to determine the denatured weights of unknown proteins using SDS gel.
Hypothesis: If gel electrophoresis is conducted on unknown proteins, then the molecular weight can be determined by the distance travelled through the SDS gel compared to the standard.
Methodology: The experiment begins with the denaturing of the proteins through heating by test tubes as well as SDS buffer. This is accomplished by separating the polypeptides within the protein samples. The SDS buffer solution is used …show more content…
a) A polypeptide is a linear polymer of amino acids, linked together by peptide bonds, whereas proteins are functional products of polypeptide bonds and other factors.
A native protein is one with all polypeptide bonds formed together in its normal, biological form. A denatured protein is one that is separated into different bands of polypeptides, and loses its specific shape and ultimately its functionality.
The purpose of electrophoresis is to use SDS gel to denature proteins and electricity to migrate the proteins through the gel. The migration is a direct correlation to the weight of each sample, and this determination is the true purpose of electrophoresis.
Two alternate factors for the way a protein migrates through gel would be its net charge and its shape. The net charge would affect how fast it repels from the location of each polarized end, and the shape’s compaction would also alter the speed.
The two functions of SDS …show more content…
In the native state, albumin would have 2,640 amino acids because by using the same method: 264,000/100amu= 2,640.
The SDS buffer denatures the proteins and ensures that they remain negative throughout the electrophoresis, as well as providing an aqueous environment for migration.
The agarose provided resistance against the protein’s migration, proving that larger proteins would move slower, and vice versa, explaining the correlation between weight and distance.
The tracking dye allows for correct measurements to be taken due to contrast in color between the sample and agarose/buffer.
The sample conjugated dye is another step in denaturing the protein, as well as acting as a visual aid in the measurement process.
Centrifuging and heating
The two are not equal because most proteins have more than one polypeptide subunit, the difference between the weights. The only way the two weights would be equal was if the native protein only had one polypeptide unit.
If two samples had the same ratio of distance to weight, they cannot be considered the same because they could consist of different
Period B- Ms. Dann
October 8, 2014
Nick D’Ambrosio, Matt Hermann, Carly Pruitt
Title: Electrophoresis and Protein Weight
Objective: The objective of this experiment was to gain a further understanding of gel electrophoresis, and to determine the denatured weights of unknown proteins using SDS gel.
Hypothesis: If gel electrophoresis is conducted on unknown proteins, then the molecular weight can be determined by the distance travelled through the SDS gel compared to the standard.
Methodology: The experiment begins with the denaturing of the proteins through heating by test tubes as well as SDS buffer. This is accomplished by separating the polypeptides within the protein samples. The SDS buffer solution is used …show more content…
a) A polypeptide is a linear polymer of amino acids, linked together by peptide bonds, whereas proteins are functional products of polypeptide bonds and other factors.
A native protein is one with all polypeptide bonds formed together in its normal, biological form. A denatured protein is one that is separated into different bands of polypeptides, and loses its specific shape and ultimately its functionality.
The purpose of electrophoresis is to use SDS gel to denature proteins and electricity to migrate the proteins through the gel. The migration is a direct correlation to the weight of each sample, and this determination is the true purpose of electrophoresis.
Two alternate factors for the way a protein migrates through gel would be its net charge and its shape. The net charge would affect how fast it repels from the location of each polarized end, and the shape’s compaction would also alter the speed.
The two functions of SDS …show more content…
In the native state, albumin would have 2,640 amino acids because by using the same method: 264,000/100amu= 2,640.
The SDS buffer denatures the proteins and ensures that they remain negative throughout the electrophoresis, as well as providing an aqueous environment for migration.
The agarose provided resistance against the protein’s migration, proving that larger proteins would move slower, and vice versa, explaining the correlation between weight and distance.
The tracking dye allows for correct measurements to be taken due to contrast in color between the sample and agarose/buffer.
The sample conjugated dye is another step in denaturing the protein, as well as acting as a visual aid in the measurement process.
Centrifuging and heating
The two are not equal because most proteins have more than one polypeptide subunit, the difference between the weights. The only way the two weights would be equal was if the native protein only had one polypeptide unit.
If two samples had the same ratio of distance to weight, they cannot be considered the same because they could consist of different