Gel

position of the restriction enzyme cut on the DNA sequence and what the size of the DNA fragment is. In week two the DNA sequences of the wild type and mutant were exposed to restriction enzymes and then agarose gel electrophoresis. The students prepared six tubes for the agarose gel. All of the pipetting was done with a P20 pipette. The controlled factors of this experiment were the amounts pipetted and the chosen restriction enzyme BsrG1. Tube A and Tube B were the negative controls, Tube…

Introduction The percentage of men over 40 using testosterone replacement therapy (TRT) tripled from 2001 to 2011¹. Newer, gel based forms of treatment have aided the ease of application and encouraged prescription rates. Along with this trend comes an increase in the data surrounding TRT. This influx adds to the large scale, randomized studies conducted on the use of TRT in hypogonadal men. TRT is widely accepted to provide relief from retarded spermatogenesis, low sex function, depression,…

The pre-cast gel was placed in a Pierce Precise Protein Gel Cassette. The gel was an 8x8 mini, 1mm thick and had 10 wells for loading samples. The inner chamber of the cassette was completely filled to the brim with 150 ml of Tris-Glycine gel running buffer, the mini tank outside was filled with the same buffer, to approximately 1/3 of the container. The protein samples were then…

By Chelex based DNA isolation, DNA was isolated from buccal cells to study the genes TAS2R38, CDK3, ADH/ALDH, and D1S80. It was hypothesized that after gel electrophoresis, the TAS2R38 DNA sample will be cleaved at two places, since by the taste test was positive for PAV. The CDK3 gene will display one or two bands and the ADH/ALDH was expected to form one or three bands based on homozygosity or heterozygosity for the gene. D1S80 will have repeated sequences from 14 to 72 repeats. After…

and this experiment illustrated the versatility of these molecular biology techniques of PCR and gel electrophoresis to serve as methods identifying individuals. Both of the hypotheses made in this experiment that (1) there were genetic variation with the six students and (2) Student 3’s genotype was heterozygous were supported by the data. As seen in Figures 1 and 2 and Table 1 with different agarose gel percentages, it can be concluded that each of the six students are genetically variable and…

experimental restriction enzyme since they believed it would make the mutant DNA linear since it would cut the plasmid once while leaving the wild-type DNA uncut and supercoiled. This would make the wild-type DNA travel faster through the agarose gel in gel electrophoresis. They also chose a positive control group with the HindIII restriction enzyme that would cut both the mutant 1 and the wild type plasmid once and at the same position. Then they had a wild type and mutant 1 plasmids that had…

genetics. Although DNA is typically used in agarose gel electrophoresis, it was not provided for this lab, so the use of three different dyes had to make do. A null hypothesis was created indicating that dyes with a lighter molecular weight would not pass through the agarose gel easier than those with a heavier molecular weight. Where as the alternate hypothesis stated that, dyes with a lighter molecular weight would pass through the agarose gel easier than those with a heavier molecular weight.…

sites for regulatory proteins that will affect RNA polymerase activity. On a related note, the size of proteins can be analyzed using SDS-PAGE (sodium dodecyl sulfate Polyacrylamide Gel). This technique is useful since it denatures and coats each protein being tested with the same charge, using β- mercaptoethanol and DTT. Gel- electrophoresis is then run to compare the sizes of different proteins. The goal of this experiment was to learn about the process of SDS-PAGE and also about gene…

article that was very captivating to me. As many of you may know, heart attacks are fatal occurrences that typically result in the death of part of a heart muscle. Recent studies at John Hopkins Medical Hospital have shown that a special sticky protein, gel that they created can help stem cells stay in or on rat hearts and restore their metabolism after transplantation. This discovery has given a new way and hope for researchers to heal the human hearts after heart attacks. This research started…

additive depends on its performance such as forming thick solution or gels. For example, κ− carrageenan forms strong and rigid gels with potassium salts, forms brittle gels with calcium salts, clears gel with sugar addition, and exhibits syneresis. On the other hand, ι− carrageenan forms elastic gels with calcium salts, clears gel with no syneresis, and creates a gel that is freeze/thaw stable, while λ− carrageenan is not gel-forming but it forms high viscosity solutions (McHugh, 2003). The…