Gel

column leaving just the DNA in its membrane. Later some Elution Buffer was used to separate the DNA from the membrane of the spin column. Day two consisted of making the plasmid DNA resistant to Kanamycin and Ampicillin. Day three we made 0.8% agarose gel and ran electrophoresis to be able to see the weight of the DNA. Day four we genetically transferred the ligated plasmid DNA to E.coli and plated the cell mixture. Day five we looked at our results and went over them. All of this works together…

Today's post is a little long, but informative. If you're interested in or curious about gel manicures read on. I was watching one of my favorite YouTubers MissHollyBerries, and the subject of gel polish came up. Madam Glam was kind enough to send her their gel manicure nail kit containing 2 gel polishes and a LED lamp. I believe this was a special set because the beginners’ kit sold on their web site (madamglam.com) contains a “Magical Pocket led lamp”, one top coat, one base coat and one…

The Brentwood Home Bamboo Mattress with gel memory foam is a unique mattress with ingredients specific to Brentwood. This review is specific to the 11-inch thick mattress in the queen size, but this mattress is available in different sizes and thicknesses. This mattress is unique due to its usage of New Zealand wool and bamboo fibers. Below, there is an in-depth discussion on this mattress. 1. 2.5 inches Gel Mattress Foam: This foam is infused with blue gel. This helps combat the heat that…

Thin Layer chromatography was also use to separate the mixture of fluorene and fluorenone. Thin layer chromatography is a useful separation method when the components of a mixture are in question. Analyzing what the mixture consist of can be efficiently determine by TLC by calculating Rf and comparing these values. For the separation and isolation of a mixtures components, this method is extensive and not as efficient at column chromatography. This conclusion is represented in the data gathered.…

placed inside the gel for two main reasons. One, the gel is a way of holding them to know where they are. Two, the migration needs to occur in a manner that allows for the separation of different-sized pieces of DNA or RNA. The gel has many microscopic holes through which the nucleic acids wiggle as they migrate within the electric field. The smaller the nucleic acid sequence, the easier it is for it to wiggle through the holes. So, smaller pieces of DNA and RNA run through the gel faster than…

Observe Ligand-Protein Interaction using Gel Filtration Chromatography (Phenol Red Binding to Bovine Serum Albumin) Introduction: Serum albumin is often known as blood albumin, and is the most abundant protein in humans and other animal plasma. Serum albumin is also known as a carrier protein by non-specifically binding several biomolecules. It is essential for maintaining the osmotic pressure that is needed for body fluids distribution between body tissues and intravascular compartments.…

If done correctly, the audience that is being targeted will adopt a new view or idea or will go buy the product in the well-rounded advertisements. In this particular instance, SANZER, a hand sanitizer brand, is trying to advertise its germ-killing gel. By use of enthymeme logic to build an ethical and emotional appeal in the visuals of its advertisement, the company has produced a marketing agent that aims to promote its product. At first glance, the audience is drawn to the focal point of the…

Abstract The objective of this lab is to analyze DNA samples using gel electrophoresis and reaction enzymes and then comparing them to the DNA found at a “crime scene” to detect a match. After the experiment was complete the Materials & Methods Part 1 To begin this part of the experiment there needs to be a couple of reaction tubes labeled 1 through 4. First 10 microliters of reaction buffer to reaction tubes 1 and 2. You will then need to add 15 microliters of suspect 1’s DNA to both tubes…

Article Comparisons to Southern (1975) Article One The article “Detection of specific sequences among DNA fragments separated by gel electrophoresis” (1975) by E.M. Southern focuses on the way that the fragments of DNA can be transferred from agarose based gels to cellulose nitrate filters. Then fragments themselves are hybridized to active RNA. E.M. Southern’s main influences for the investigation were the studies of Smith and Wilcox (1970), and Kelly and Smith (1970), which showed that the…

Electrophoresis; Separation of Proteins Purpose: To create an electrophoresis gel in order to further separate Cytochrome C, Myoglobin, Albumin and Hemoglobin(proteins) based off of their net charges. Materials: • Rabbit hemoglobin • Serum albumin(cow) • Myoglobin • Cytochrome C • Microtubes • Tape • Micropipets • Agarose gel • Buffer • Gel chamber • Test tubes • Test tube holders • Gloves • Macropipets Properties of Proteins: Protein Color Isoelectric Point Net Change at pH 8.6 Cytochrome C…