Lactate dehydrogenase was assayed according to the method of King (1965) [21]. Reagents required: 1. 0.1 M glycine buffer: 7.5 g of glycine and 5.85 mg of sodium chloride were dissolved in one litre of distilled water. 2. Buffered substrate: 2.76 g of lithium lactate was dissolved in 125 ml of glycine buffer containing 75 ml of 0.1 N sodium hydroxide solution. This was prepared just before use. 3. 0.4 N NaOH. 4. 5.0 mg of NAD+ was dissolved in 1.0 ml of distilled water just before use. 5.…
Freezing salt-water experiment Contents: Contents, introduction and literature review on page 1. Aim, hypothesis, variables, risk assessment, materials and method on page 2. Results on page 3. Discussion and conclusion on page 4. Introduction: I have decided to do my SRP on salt water freezing because our planet is 71% water and about 96.5% of it is in the ocean which it is salt water so this is why I was interested in seeing how much salt, depresses the water from freezing. Literature…
Adsorption isotherm models : To study the proper model, the isotherm data were analyzed and were fitted them into two models, Freundlich and Langmuir, because they are the most suitable and employed models. Equilibrium uptake of CV and MB was investigated with sorbent weight of 0.5 g of fennel seeds in contact with 100 mL of solutions and at pH 4 for CV and 6 for MB. The flasks were shaken at 200 rpm and the equilibrium concentration of the remaining CV and MB was determined by spectrometer.…
Ecology Research Laboratory are the authors of Acid Rain and its Ecological Consequences, a review addressing the issue of acid rain precipitation. The article hypothesizes, “Acid rain is one of the most serious environmental problems emerged due to air pollution” (Agrawal & Singh, 2008). It was assumed that humans have harnessed and utilized different natural resources for their individual benefits from the onset of civilization to the present day. In turn, acid rain has become a major…
calcium carbonate is dissolved in a known amount of hydrochloric acid and is then titrated against sodium hydroxide. An orange colour will be observed at the end point. This experiment is a success as two results obtained from the experiment were relatively close furthermore, an average of 21.50% of calcium carbonate obtained is close to the literature value of 20%. 2. Introduction There are several…
Lowry independently expanded the Arrhenius acid definition into one of his own. The Bronsted-Lowry acid is a molecule or ion that is a proton donor. Lewis's definition emphasizes the role of electron pairs in acid-base reactions. A Lewis acid is an atom, ion or molecule that accepts an electron pair to form a covalent that accepts an electron pair to form a covalent bond. Each theory agrees that an acid is a substance that is dissolved in water and is separated. Although…
DETERMINATION OF STOMACH ACID NEUTRALIZATION USING ANTACIDS Authors: Alexxis Harris Constance Doty Brandon Hokenson Gavin Hunt Melissa Munoz Affiliation: Department of Chemistry, University of Central Florida Abstract The purpose of this experiment is to identify which antacid is the best at neutralizing stomach acid. Antacids are commonly used self-prescribed medications. The stomach produces hydrochloric acid, which has a…
Discussion Fold purification was calculated from specific activity. From sample A to B there was the addition of MgCl2 which denatured some proteins that were removed in the pellet. The second step of purification was even more successful with a 4.1 fold increase in purification, it was achieved by denaturing the non-recombinant E.Coli proteins. The Second step was much more beneficial to the total purification but the combination of the two steps achieved an even better purification of 4.6…
The experiment goal of the lab is to determine the concentration of acetic acid (CH3CO2H) in the commercial white vinegar brand. Procedure and Data Statement The complete experimental procedure is available in the General Chemistry Laboratory Manual for CSU Bakersfield, CHEM 211, pages 60-61, 64-65. Experimental data recored on the attached data pages 62-63, 66-68. Discussion…
Using qualitative analysis, two cations in an unknown solution was identified. After repeated mixing, centrifugation, and decanting, lead was confirmed in Part A and barium in Part B. In Part C, different colored flames of metal species were observed using cation flame tests. Formula unit, total ionic, and net ionic equations were then composed for the identified cations. If the experiment were to be repeated, step 13 could be ommitted as barium was already confirmed in step 12 with the…