Gram-positive cocci are an interesting group of bacteria to study. Some common species include Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Streptococcus pyogenes, and Sarcina lutea. Gram-positive cocci have a gram positive cell wall, cocci morphology and many different styles of grouping. Some species have begun to exhibit a resistance to fluoroquinolone by the use of altering the drug binding site and the use of a membrane pump (Hooper 2002). Thanks to the…
Fleming had been culturing colonies of Staphylococcus when one of the plates, untreated by Lysol, had been contaminated by Penicillium notatum. Fleming observed that the growth of staphylococci had been inhibited in the area immediately surrounding the mold, suggesting that the mold secreted an antibacterial…
Identifying environmental reservoirs of methicillin-sensitive Staphylococcus aureus (MSSA), as well as Methicillin-resistant Staphylococcus aureus (MRSA) in the community might be critical for controlling the spread of community–acquired staphylococcal infections [27]. Dental unit waterlines are known as reservoirs since they are frequently contaminated…
Biographical Sketch of Teresa Burton Teresa Burton, born in New Jersey and raised in Virginia, in May of 1998 graduated with a Diploma in Nursing from Southside Regional Medical Center (SRMC), Professional School of Nursing in Petersburg, Virginia. The SRMC School of Nursing presented Teresa with The Lettie Pate Whitehead Foundation Scholarship Award, which is a grant for the education of deserving female students with financial needs for education. During that period, she worked as a…
Introduction Clinical laboratories have relied historically on phenotypic methods (i.e. culture and biochemical tests) to identify microorganisms. This can be time consuming task especially with slow growing organisms such as Mycobacterium tuberculosis. Molecular methods are now well established in the diagnosis of infectious disease. . (Harwood 2012). For the purpose of this essay I will give a brief overview of both traditional and molecular methods that re used today. I will focus on the pros…
found this article very engaging yet descriptive. Especially when she explained that not all bacteria are bad for you, and then continues to state some are very helpful. For instance she touches base on Staphylococcus epidermis, and how it shields us by taking up the space that Staphylococcus aureus would otherwise conquer. It’s intriguing to now know that acne (Acne vulgaris) the most common skin bacterium, works the same way. Acne vulgaris invades certain parts of our body to hold back other…
Extraction was performed by using Soxhlet apparatus with 95% (v/v) ethanol. The dried extract was dissolved in Dimethyl Sulfoxide (DMSO) and used to assay the antibacterial activity against Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853. An ointment was prepared from the ethanol extract (10% w/w) and assessed for its in vivo wound healing potential on infected rat model by rate of healing, bacterial count, biochemical analysis…
The objective of this lab was to isolate and identify an unknown Gram positive and Gram negative bacteria through the use of various microbiological techniques. The identification of the genus and species for the unknown culture should be obtained based on the characteristics for Gram positive and Gram negative bacteria. Bacteria are prokaryotic microorganisms that lack a nucleus and therefore all its genetic information is stored in the cytoplasm of the cell. The bacterial cell envelope…
negative Staphylococcus aureus, Enterococcus, Enterobacteriaceae family species and anaerobes including Clostridium species. In light of the defined organisms, many authors had driven the work to find out the actual micro biota of snakes in many parts of the world. In view of the severity of the snakebite, several hypotheses explained for the infectious wounds originate after the snakebite. The secondary wound infection involve a variety of bacterial flora includes Staphylococcus aureus, Proteus…
5% sodium chloride. The medium is differential because it differentiates and isolates Staphylococcus aureus from other Staphylococcus species. Staphylococcus aureus produces acid from mannitol fermentation by producing a yellow halo around the growth. Other Staphylococcus species will grow, but it will not ferment the mannitol. The medium is selective because it has a salt concentration, which allow Staphylococcus species to grow since they are used to living in high salt concentrations, for…