Introduction
The infection at the wound site is a severe kind of problem and it delays regeneration of epidermis and dermis in the wound and slows wound closure. Due to the secretion of microbial enzymes by wound pathogens, a variety of extracellular matrix proteins were degraded. Synthetic antimicrobial therapy used in the wound management and eradication of pathogens. However, it has many shortcomings such as anti-microbial resistance, cyto-toxicity against host tissue and absence of synergistic activity. In order to overcome these limitations, Pyto-pharmaceuticals extracted from herbal plants were applied to manage the wound infection and treatment. The objective of this work is to evaluate the wound repair and regeneration potential of the fruits of Terminalia bellirica which has a variety of pharmacological activities such as astringent, antiseptic and laxative.
Materials and methods …show more content…
Extraction was performed by using Soxhlet apparatus with 95% (v/v) ethanol. The dried extract was dissolved in Dimethyl Sulfoxide (DMSO) and used to assay the antibacterial activity against Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853. An ointment was prepared from the ethanol extract (10% w/w) and assessed for its in vivo wound healing potential on infected rat model by rate of healing, bacterial count, biochemical analysis, and expression of matrix metalloproteinase. In addition to that, the collagen content in the granulation tissue was estimated to comment on wound regeneration potential of the fruits of Terminalia
The infection at the wound site is a severe kind of problem and it delays regeneration of epidermis and dermis in the wound and slows wound closure. Due to the secretion of microbial enzymes by wound pathogens, a variety of extracellular matrix proteins were degraded. Synthetic antimicrobial therapy used in the wound management and eradication of pathogens. However, it has many shortcomings such as anti-microbial resistance, cyto-toxicity against host tissue and absence of synergistic activity. In order to overcome these limitations, Pyto-pharmaceuticals extracted from herbal plants were applied to manage the wound infection and treatment. The objective of this work is to evaluate the wound repair and regeneration potential of the fruits of Terminalia bellirica which has a variety of pharmacological activities such as astringent, antiseptic and laxative.
Materials and methods …show more content…
Extraction was performed by using Soxhlet apparatus with 95% (v/v) ethanol. The dried extract was dissolved in Dimethyl Sulfoxide (DMSO) and used to assay the antibacterial activity against Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853. An ointment was prepared from the ethanol extract (10% w/w) and assessed for its in vivo wound healing potential on infected rat model by rate of healing, bacterial count, biochemical analysis, and expression of matrix metalloproteinase. In addition to that, the collagen content in the granulation tissue was estimated to comment on wound regeneration potential of the fruits of Terminalia