Saccharomyces cerevisiae

Chronological lifespan of S. cerevisiae mutants lacking telomere capping protein Cdc13 and Exo1 Lay summary For over 25 years, yeast has been used as a model to understand cellular processes such as how; DNA is repaired and produced, also for understanding different aspects of the cell lifecycle and principle molecular processes in eukaryotes (the same type of cells that make up humans, animals and plants). Because yeast can be easily grown until it dies in a relatively short time, it is an…

growth, we looked at Saccharomyces…

messenger rna the structure of messenger rna : Messenger RNA functions as the template for protein synthesis it carries genetic information from DNA to a ribosome and helps to assemble amino acids in their correct order. Each amino acid in a protein is specified by a set of three nucleotides in the mRNA, called a codon. Both prokaryotic and eukaryotic mRNAs contain three primaryregions .The 5 untranslated region is a sequence of nucleotides that is at the 5 end of the mRNA and does not code for…

An experiment was performed to analyze the effects of heat shock in yeast called Saccharomyces cerevisiae, to assess the hypothesis that yeast extracts up-regulate gene expression during heat shock the same way bacteria cells up-regulate gene expression during heat shock. In bacteria cells, heat shock causes the bacteria to up-regulate gene expression and produce more proteins. Similarly, gene expression and protein production increases in yeast during heat shock. Therefore, the experiment was…

The aim of this experiment was to analyze a sample of finished beer for possible microbiological contaminants such as lactic acid bacteria, Gram-negative bacteria, and wild yeasts/molds. This was accomplished by using a series of spread/pour plate dilutions with various media types that contained selective ingredients. By cross-referencing the different medias and comparing observations, the generalized groups of contaminants were able to be confidently conjectured. Growth was witnessed on DRBC,…

Eukaryotes have much larger genomes than prokaryotes, and therefore, must condense their DNA into chromatin. Chromatin is composed of histone proteins that help to condense and organize the DNA forming chromosomes. The basic unit of this chromatin is a nucleosome, which contains about 150 base pairs of DNA that are wrapped 1.7 times around the core histone proteins. However, this tightly wrapped chromatin becomes a problem when regulatory proteins need access to specific sections of the DNA.…

Despite indispensable, the number of essential genes is composed to be the minority in an organism. In the single-celled Saccharomyces cerevisiae (yeast) genome, the whole genome systematic gene knockout analysis found 18.7% genes that are essential for viability (GIAEVER et al. 2002). There are around 25% in Drosophila (DIETZL et al. 2007). In Caenorhabditis elegans (C. elegans), it is estimated that there are ~15% of the total genes in the genome are essential genes (JOHNSEN AND BAILLIE 1997),…

Summary Baker’s yeast (Saccharomyces cerevisiae) is a unicellular organism, classified as a fungus, or an organism that lives and receives its nutrients by decomposing the organic material in its surroundings. This species is easy to use throughout laboratory tests because of their quick generation times, implementing the use of budding as a form of asexual reproduction. The naked eye can easily view the colonies that these yeast cells form, and carefully count them to determine the growth. The…

Subsequently, other families of GFP variants were discovered and GFP mutants were engineered. These families of GFP-like proteins were discovered in a class of non-bioluminescent Anthozoa (corals). These GFP-like variants have differing properties with regards to chromophore, excitation and emission wavelengths (Tsien & Tsien, 1998) e.g. red & later in other coral species - yellow, cyan and orange. The only other species that fluoresces green is Renilla reniformis (Matz et al., 1999).…

The recombinant hepatitis B vaccine is produced from genetically engineered Saccharomyces cerevisiae, a yeast. The vaccine is noninfectious, and it contains hepatitis B surface antigen (HBsAg) (Keating & Noble, 2003). The first recombinant hepatitis B vaccine, Recombivax HB, was approved by the Food and Drug Administration in 1983, followed by Engerix-B in 1989 (Hepatitis B Vaccine Safety, n.d.). safety. The Vaccine Adverse Event Reporting System (VAERS) is one way that the Centers for Disease…