Summary Baker’s yeast (Saccharomyces cerevisiae) is a unicellular organism, classified as a fungus, or an organism that lives and receives its nutrients by decomposing the organic material in its surroundings. This species is easy to use throughout laboratory tests because of their quick generation times, implementing the use of budding as a form of asexual reproduction. The naked eye can easily view the colonies that these yeast cells form, and carefully count them to determine the growth. The goal of the mutagenesis experiment is to observe the effect that a UV light will have on a culture of yeast cells in two different agars, testing the rate of mutations and cell death that occurs. The overall purpose of the experiment is to connect the
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The experiment implemented the use of yeast cells with the trpl-289 gene, which inhibits their ability to grow without the presence of tryptophan. The plates underwent UV tests on two different types of agars, synthetic complete medium (SC) and synthetic dropout medium (SD). Each agar had seven separate petri dishes that contained a 10-4 dilution. The seven petri dishes of the SC and seven petri dishes of SD agars were exposed to differing amounts of UV radiation, each time interval including one SD and one SC plate. The cultures were allowed to grow for one week, and then were counted in order to compile the class data. It was found that exposure of the SD plates to radiation causes some of the yeast cells to revert back into their original state, allowing them to grow without the presence of tryptophan, while exposure to the UV rays killed many of the cells on the …show more content…
Two plates, one SD and one SC, were labeled no irradiation, and were not exposed to any UV rays. Each of the plates were labeled accordingly, and had one SD and one SC per time interval; 20, 40, 60, 80, 100, and 120 seconds. Two spaces underneath the short-wave UV lamp were marked to ensure that each of the plates were placed in the exact same position for each test. Before irradiation, the lids of the plates were removed and replaced with Saran Wrap. Once the irradiation was completed for the correct time interval, the Saran wrap was removed and the lids were replaced. Then the plates were stacked and taped together so to be incubated for two
The experiment implemented the use of yeast cells with the trpl-289 gene, which inhibits their ability to grow without the presence of tryptophan. The plates underwent UV tests on two different types of agars, synthetic complete medium (SC) and synthetic dropout medium (SD). Each agar had seven separate petri dishes that contained a 10-4 dilution. The seven petri dishes of the SC and seven petri dishes of SD agars were exposed to differing amounts of UV radiation, each time interval including one SD and one SC plate. The cultures were allowed to grow for one week, and then were counted in order to compile the class data. It was found that exposure of the SD plates to radiation causes some of the yeast cells to revert back into their original state, allowing them to grow without the presence of tryptophan, while exposure to the UV rays killed many of the cells on the …show more content…
Two plates, one SD and one SC, were labeled no irradiation, and were not exposed to any UV rays. Each of the plates were labeled accordingly, and had one SD and one SC per time interval; 20, 40, 60, 80, 100, and 120 seconds. Two spaces underneath the short-wave UV lamp were marked to ensure that each of the plates were placed in the exact same position for each test. Before irradiation, the lids of the plates were removed and replaced with Saran Wrap. Once the irradiation was completed for the correct time interval, the Saran wrap was removed and the lids were replaced. Then the plates were stacked and taped together so to be incubated for two