Saccharomyces cerevisiae

Presence of AOX1 promoter which is strong and controlled by the induction of methanol is used in recombinant antibody production. An amino terminal signal sequence, commonly S. cerevisiae alpha factor, is required for secretory production of antibody which later undergoes proteolytic cleavage by endo-protease KEX2 for proficient release of immunoglobulins. There is limited data regarding expression of a whole immunoglobulin G. Dimeric scFV-Fc antibodies in P. pastoris attained expression levels of 10-30mg/L. YEAST EXPRESSION SYSTEM FOR THE PRODUCTION OF INTERFERON Plasmid containing cDNA of human interferon gamma is kept under the control of 3-phosphoglycerte kinase promoter in the yeast Saccharomyces cerevisiae. The amount of of expression was greater under PGK promoter than just the IFN-γ mRNA. 25 x 10 (6) units per litre culture of active IFN-γ are synthesized in transformed…

pH of the environment in which they taker place. Because fruits contain a high sugar content, it was theorized that the extracts of these fruits could be used as a source of energy for Saccharomyces cerevisiae. By measuring the rate of fermentation of the yeast given these fruit extracts, the relative acidities of these fruits could be tested. One method to measure the rate of fermentation is to use gas pressure sensors to measure the rate of gas production by the yeast while sealed from the air…

solve the issue, and therefore, it was my first lesson in trouble shooting a solution to solve the problem. It was an honor to have my first research experience to be in this lab because everyone worked closely as a team to solve scientific questions. The lab always provided a supportive and collaborative environments to help each other solve experimental challenges. This experience was pivotal in gaining trouble shooting skills, a critical skill required for scientific research, and was…

What is yeast? To most people yeast is just something that is in the food that we eat; However to the scientific community yeast is much more than that. According to the Dictionary of Student Science yeast is a one-celled fungi that can cause the fermentation of carbohydrates, producing carbon dioxide and alcohol. ( ) Yeast can be found in the soil, water, on the surface of plants as well as on the skin of humans. Like other fungi, yeasts gets its food from the organic matter around them. The…

Thank you for you information request on Florastor® (Saccharomyces boulardii) and prevention of Clostridium difficile. As you stated earlier, Ms. JS was recently transferred from another facility to Llano Estacado Estates for a higher level of care. JS is an 84 year old, female patient that has been diagnosed with Alzheimer’s dementia, anxiety, depression, insomnia, hypothyroidism, constipation, and gastro-esophageal reflux disease (GERD). She is currently on the following medications: Aricept®…

Fermentation, the process that follows cellular respiration, uses a series of redox reactions to produce ATP without the presence of oxygen (anaerobic respiration). This process produces significantly less ATP than aerobic cellular respiration. There are two types of fermentation-lactic acid fermentation and alcoholic fermentation. In this experiment, the main focus will be on alcoholic fermentation. Alcoholic fermentation results in the production of ethanol and carbon dioxide. The carbon…

Effects of Different Concentrations of Caffeine on Cellular Respiration in a Saccharomyces Cerevisiae Cell Valentine Bedford Red Deer College- Red Deer Results The negative control showed no release of CO2 gas. The positive controls (0.1 and 0.5) showed positive results that the caffeine did not inhibit, but increase the CO2 amount measured in the Durham tubes. The CO2 gas released peaked at the 1.5 mL concentration of caffeine, but dropped when the caffeine concentration increased to 2.0 mL.…

Designing different experiments regarding the same concept to investigate this topic further would be interesting. One experiment that could be done in the future could be using cold water instead of warm water. In this experiment the students studied how warm water affects the yeast, and learned that warm water activates the yeast enzymes. Where cold water may denature the enzyme, as the students previously reviewed that three factors that affect enzyme activity are temperature, concentration…

Ultra-violet light is produced by high-energy wave frequencies just outside of the visible spectrum. This light energy can interfere with the bonds which comprise the backbone of DNA by exciting the electrons in these atoms—leading to conformational changes which cause base-pair bonds to be broken, shifted, or replaced, causing genetic mutations, or abnormalities in the typical phenotypic expression of an organism. The purpose of this lab was to understand more about ultraviolet light as an…

Supplements containing yeast, such as Saccharomyces cerevisiae, has shown to decrease microbial change. Faubladier et al. found that this particular yeast was able to survive the gastrointestinal tract, but no more was found after discontinuing supplement (Faubladier et al. 2013). An earlier study done by McDaniel et al. found that Saccharomyces cerevisiae was not able to promote better digestibility (McDaniel et al 1993). In contrast, more recent studies that used cannulated horses on a high…