Catechol Oxidase Lab Report
To conduct the experiment to see the effects of varying temperatures on catechol oxidase reaction time, multiple water baths were prepared at four temperatures: zero degrees, thirty-seven degrees, eighty degrees, and room temperature. The rate of the catechol oxidase reaction was measured by the use of a spectrophotometer.
Next we gathered the materials that we need: gloves, spectrophotometer, outlet, eight test tubes, four test tube rack, ice, three thermometers, parafilm, masking tape, disposable uncalibrated pipettes, pipette pumps, kimwipes, a sharpie, absorbance conversion table, one percent cathechol, potato extract, DI water, two large test tubes and a timer. The two large test tubes contained potato extract and catechol. The control group was the blank test tubes, the independent variable was the absorbance rates, and the control variable was the temperature, volume, and pH.
Gloves were used to prepare the blank test tubes by placing a label ‘blank’ on the tube. The solution contained 0.5 ml of potato extract and 5.5 ml of deionized water. The other four test tubes were prepared with 0.5 ml of potato extract and 5 ml of deionized water for each individual test tube and labeled one through four. The four new test tubes with 0.5ml of catechol were labeled C1, C2, C3, and C4. Finally, these eight test tubes were placed into a water bath and measured from lowest to highest temperatures. The test tubes were measured every five minutes, for example…