preliminary data for TARA that shows the technique is sufficiently sensitive to detect 0.005 fmoles of synthetic RNA template by eye and/or a smartphone scanner using transfer reaction molecular amplification and GNP signal amplification alone on a lateral flow strip (See Figure 3). Moreover, there was no background signal from no template control (non-templated transfer, Figure 3A) or a single base mismatch RNA template (Figure 3C). The reaction conditions are 150 nM A probe, 100 nM G probe conjugated with GNP incubated at room temperature for 30 minutes in the CLBuffer. Detection is linear with a correlation coefficient r2 of 0.93 at this low detection range. The test line bands were quantitated by scanning in grayscale with VueScan App (Harrick Software) on iphone 6 to a jpeg file which was opened and quantitated in Adobe Photoshop v.CS5.1. In order to lower costs, we propose the use of…
I have a love/hate relationship with Pinterest. I signed up after being egged on by my two sisters who both enjoyed collecting pins at the time. I've lost track of my younger sister's activity-being a social butterfly she's most likely moved on to bigger and better things, but my older sister persisted-especially after the arrival of her first child. I pinned at first with enthusiasm, but the pressure to complete pins I'd added to my boards began to haunt me, and a few feeble recipe attempts…
\THE YOGABRAIN® STORY While participating in a Sun Salutation Practice being run in Brisbane, Australia, Henry had a chance to chat with Alana who was running the practice. We talked about how hard it was for both student and teacher to count the full 108 rounds of the practice session. Since you were having to keep count of the rounds, this did not allow you to enter the flow state of mind. Instead, you had to constantly work to remember where you were in the count. Is There an App for this?…
1. I have completed my survey for the Information Technology Infrastructure Library Course and have received my passing score 26\40. 2. Kim Rymsha’s computer is back in her office and both her personal and network printers have been connected to her Pc. 3. Docsvault has been completely installed on Lisa Cabrera. 4. I have unlocked Angel Li, of Fiscal, 10th Floor Active Directory Account. 5. I am awaiting the status for pickup of our DBH Inventory of old Equipment that has been updated for…
Life holds a series of setbacks; every day we deal with problems that affect us on a personal level. In my four years of college, I have definitely had my fair share of setbacks through athletics and my living arrangements at Saint Peter’s University. My freshman year, I came from a slow, quiet suburban childhood into the fast and nonstop environment of Jersey City. The following year, I tried to take on more responsibility by renting a house near campus with my teammates, but I ended up stuck…
POP! Your ACL just snaps in half. The pain is unbearable. It feels like your knee is just hanging because nothing is holding the back part of your knee together. You are scared to get the MRI results back because it is a huge setback. “You’ve torn your ACL.” Those are words an athlete never wants to hear. What is Your ACL? You may be wondering what your ACL is and what it does? Your ACL is the Anterior Cruciate Ligament. It is one of the four ligaments that stabilizes your knee so there is no…
ground. There, on the board of their fifth hour class, lies the words in big bold letters, TEST WEDNESDAY. The following minutes are filled with stress, anxiety, and worry. But this does not compare to the time consuming, mind thwarting, brain exhausting hours of study5ing that is now to commence to do well on this test. That day after…
adult whether it was my GPA or my score on a test or the score they give a new born baby on how fast they cry or react to a stimulate. For once in my life I would want something else to define me rather than just a couple of numbers. As little kids barely entering school we are encourage to do our best. We are inspired and rewarded when we do something right but, as teenagers and adults we are required to know things already. When I was in elementary school we would have to do an exam that the…
The counselor began to mention the aptitude tests and with new light shed on the subject the client was more than willing to continue with the test in order to build her packet together for new employment. The counselor used active listening strategies and was empathizing with the client in the midst of her irrational thoughts. The counselor also utilized cognitive strategies to change her negative thinking into more constructive thoughts. While many different crises were presented, the…
Why Test When You Can Assess? According to American Test Anxieties Association, “40-60% of students admit to having test anxiety at some point or another and around 38% of students record having it all the time” (Brideau par. 5). Exams should be outlawed in favor of another form of assessment, such as demonstration, class activities, and projects because hands on assessments give a better explanation to students and improves motivation, helps learners improve their performances, and also…