preliminary data for TARA that shows the technique is sufficiently sensitive to detect 0.005 fmoles of synthetic RNA template by eye and/or a smartphone scanner using transfer reaction molecular amplification and GNP signal amplification alone on a lateral flow strip (See Figure 3). Moreover, there was no background signal from no template control (non-templated transfer, Figure 3A) or a single base mismatch RNA template (Figure 3C). The reaction conditions are 150 nM A probe, 100 nM G probe conjugated with GNP incubated at room temperature for 30 minutes in the CLBuffer. Detection is linear with a correlation coefficient r2 of 0.93 at this low detection range. The test line bands were quantitated by scanning in grayscale with VueScan App (Harrick Software) on iphone 6 to a jpeg file which was opened and quantitated in Adobe Photoshop v.CS5.1. In order to lower costs, we propose the use of…
I have a love/hate relationship with Pinterest. I signed up after being egged on by my two sisters who both enjoyed collecting pins at the time. I've lost track of my younger sister's activity-being a social butterfly she's most likely moved on to bigger and better things, but my older sister persisted-especially after the arrival of her first child. I pinned at first with enthusiasm, but the pressure to complete pins I'd added to my boards began to haunt me, and a few feeble recipe attempts…
\THE YOGABRAIN® STORY While participating in a Sun Salutation Practice being run in Brisbane, Australia, Henry had a chance to chat with Alana who was running the practice. We talked about how hard it was for both student and teacher to count the full 108 rounds of the practice session. Since you were having to keep count of the rounds, this did not allow you to enter the flow state of mind. Instead, you had to constantly work to remember where you were in the count. Is There an App for this?…
1. I have completed my survey for the Information Technology Infrastructure Library Course and have received my passing score 26\40. 2. Kim Rymsha’s computer is back in her office and both her personal and network printers have been connected to her Pc. 3. Docsvault has been completely installed on Lisa Cabrera. 4. I have unlocked Angel Li, of Fiscal, 10th Floor Active Directory Account. 5. I am awaiting the status for pickup of our DBH Inventory of old Equipment that has been updated for…
Life holds a series of setbacks; every day we deal with problems that affect us on a personal level. In my four years of college, I have definitely had my fair share of setbacks through athletics and my living arrangements at Saint Peter’s University. My freshman year, I came from a slow, quiet suburban childhood into the fast and nonstop environment of Jersey City. The following year, I tried to take on more responsibility by renting a house near campus with my teammates, but I ended up stuck…
POP! Your ACL just snaps in half. The pain is unbearable. It feels like your knee is just hanging because nothing is holding the back part of your knee together. You are scared to get the MRI results back because it is a huge setback. “You’ve torn your ACL.” Those are words an athlete never wants to hear. What is Your ACL? You may be wondering what your ACL is and what it does? Your ACL is the Anterior Cruciate Ligament. It is one of the four ligaments that stabilizes your knee so there is no…
Thou Shalt Attend In high school it was quite apparent that students could receive many absences with little or no consequences and still be able to maintain a good grade point average. However, in college that is not the case. Student attendance in college is important because students who attend class have better grades, have better relationships with their peers as well as professors and develop responsible habits and behaviors. Students who attend class often receive better grades on tests…
responses in the chart papers; the group of students who received drama-based teaching would use pencil and loose lifts to write down their scripts during the creative drama activity. After the teacher finished the lessons, both of two groups of participants on Day 1 and Day 2 took a test to measure their reading comprehension skills of the story by approaching different teaching instructions. There were 40 copies of the test for both groups of students. The test contained 20 multiple choices…
today experience stress much more than their parents. It can manifest in many different ways. Test anxiety if left untreated can be debilitating for the student. As a teacher, we should foster a safe environment for students to learn. If a teacher could change simple teaching techniques could it lower student test anxiety? Are there coping mechanisms that can be taught to a student that can be used successfully during high anxiety testing situations? The purpose of this study is to test…
be no way to solve problems. For the Candor faction, their rules state that “1. the state or quality of being frank, open, and sincere in speech or expression; candidness. 2. freedom from bias; fairness; impartiality” (Roth, Bonus Materials 28). The people in Candor are forced to share their deepest secrets with everyone else in their faction, whether they know them or not. If they choose not to, they become Factionless; which is the same as being homeless. They can’t keep any of their secrets…