Incubation period

I. Introduction A. Common and Scientific Names 1. Common Names: anhinga, darter. Also known as: snake bird, water- turkey, and American darter. a. The word of anhinga comes from Brazilian Tupi Language which means devil bird or snake bird (Encyclopedia of Life). 2. Scientific Name: Anhinga anhinga 3. Anhinga anhinga Sub-species a. Anhinga anhinga leucogaster (Viellot 1816; Nelson 2005) b. Anhinga anhinga anhinga (Linnaeus 1766; Nelson 2005) B. Geographic Range 1. Southeast United States to…

1-[Cyano(4-methoxyphenyl)methyl]cycloalkanol (VF-I) synthesized through the reaction of p-methoxyphenylacetonitrile and cyclohexanone using aq. NaOH and tetrabutylammonium hydrogen sulfate as PTC. NaBH4 and CoCl2 in 2:1 THF: H2O used for the reduction of VF-I to obtain 1-(2-amino-1-(4-methoxyphenyl)ethyl)cyclohexanol (VF-II). Further, venlafaxine derivative of the amino acid -carboxamide (VFCA-I toVII ) synthesized through coupling of N-Phthaloyl amino acid chloride derivative (2a-2g-…

The Transfer of the pGLO plasmid into Escherichia coli from the DNA of Aequorea victoria Introduction The goal of this lab is to be able to take the gene responsible for allowing organisms to glow in the dark, called Green Fluorescent Protein (GFP), located in the pGLO plasmid, and move it from the DNA of the Aequorea victoria to bacteria, Escherichia coli. The pGLO plasmid is found naturally in some organisms such as the A. victoria,…

bacteria; to understand how the process of transformation occurs, as well as the biological results and consequences that come of transformation. In order to correctly perform this lab, plasmids, agar plates, Escherichia coli colonies, as well as incubation periods were used. The data that was collected was completely opposite than what the actual outcome should have been. Yes there was growth in the agar plates however there was not as much growth as predicted. Another problem that arose was…

Escherichia coli (E. coli) is one of the most widely studied and understood living organisms. It has been used for decades in furthering understanding of gene functionality and gene-protein mechanisms for prokaryotes and eukaryotes alike (Riley et al. 2006). Prokaryotes, like E. coli, have their DNA in the form of a plasmid. A plasmid is a circular ring of DNA. The plasmid intended to transform the E. coli colonies for the purposes of this study was the pGLO plasmid. The pGLO plasmid contains…

Raw material: Two sweet varieties of Apricot (Prunus Armeniaca L.) namely Marghulam and Halman fully ripened fruits were collected from Gilgit Baltistan region in Northern Areas of Pakistan. Apricots were sun dried under open condition (26-34+4 C, RH 41-52%) for 16-19 days. The sun dried Apricots were packed in polyethylene bags containing 01Kg of dried fruit. Further The dried samples obtained were ground to a fine powder and stored at 5°C in air-tight containers prior to further analysis.…

THP-1 cells and THP-1 cell culture methodology were also provided by Ondek Pty Ltd. THP-1 cells were cultured in RPMI-10 culture medium. The first THP-1 cell culture was made by Dr Senta Walton, with a starting concentration of 2 x 105 cells/mL. The cell culture was then incubated at 37°C with 5% CO2 and subcultured every 2-3 days, when the cell concentration reached approximately 8 x 105 cells/mL. THP-1 cell based assay THP-1 cell based assay was performed to determine the immune modulatory…

Methodology 1. Sampling of the material into various study groups and control group. 2. Creating environmental conditions to mimic a forensic scene. 3. Deriving of pulp & storage of samples. 4. Isolation of DNA from pulp using phenol extraction. 5. DNA quantification and quality assessment using nano spectrophotometry. 6. Obtaining primers & its design. 7. Polymerase Chain Reaction & procedure. 8. Result analysis using Gel Electrophoresis. 9. Statistical data analysis 1) Sampling: Extracted…

The Bubonic Plague (1330-1351) in Europe has influenced many things, especially in literature. One such work of literature is The Decameron, written by a man who lived during the time of the Bubonic Plague in Florence, Italy named, Giovanni Boccaccio. Giovanni Boccaccio was born in Paris, France, in 1313 to a businessman and a Frenchwoman. Boccaccio was taken to Florence, Italy by his parents when he was an infant and was sent to Naples, in 1328, to study “commerce in the office of his…

oxygen-enriched environment, achieved by allowing ultrapure (99.98%) oxygen to flow through the system for several minutes before sealing the chamber with the Hg lamp on and the sample on a heating stage held at 150 °C for 60 min, followed by a 30-min period of incubation with the lamp off and the sample cooling to room temperature. After the ozone clean, samples were cleaned ultrasonically for 20 min in deionized water, followed by 20 min in…