Introduction Determining the identity of microorganisms is an important part of diagnosing patients and creating new medications to treat illnesses. The purpose of this report is to explain how this can be done and provide some important information about the results. Methods and Tests The techniques used were performed as directed by the student’s lab manual unless otherwise stated (Gero, McDonald, Salsgiver, & Thoele, 2011). In this experiment some of the methods learned in class,…
An unknown sample #18 was obtained on November 6, 2017. The sample came in a glass test tube with a cotton swab that contain the unknown sample. To find the identity of the unknown sample, we had to conduct several biochemical tests to eliminate and narrow down possible organisms that fit the biochemical description to accurately identify our unknown organism of interest. These biochemical tests provided insight on the physical color retention of our unknown sample, and through’s the unknown…
Gram staining was used to distinguish the unknown microbes between gram-positive microbes from gram-negative microbes. Gram-positive microbes contain a thick peptidoglycan with only one outer cell membrane, thus dying the microbes dark purple. Gram-negative microbes contain a thin peptidoglycan with an outer and inner cell membrane, dying the microbes pink when a gram stain test is done. Microbes A and C were gram- positive because of purple dye seen under the microscope. Microbe B was a…
Case Study 1 Vivek Poudel University of Texas at Arlington In partial fulfillment of the requirements of N5315 Advanced Pathophysiology Mary Schira Ph.D., RN, ACNP-BC September 19, 2017 What causes systemic lupus erythematosus (lupus)? Why did the NP suspect that Sharon may have lupus? (15 pts) Exact cause of systemic lupus erythematosus (lupus) is not known. It is believed that some people are born with tendency to develop lupus that is triggered by a viral infection, chemical…
This patient should treat by medications of all abnormal situation because she has Hyperkalaemia, high Creatinine and urea and some electrolytes low such as Na and HCO3. Suggest what the results may look like after one of the common treatments such as water, electrolytes balance, some medication, patient health improve if not may progress to renal failure and she need to Renal dialysis includes renal dialysis, peritoneal dialysis, chronic peritoneal dialysis and kidney transplantation. There is…
- In multiple myeloma, mild hemolytic anemia can be seen, whereas in SPB the hemoglobin levels are normal. Elevated creatinine and blood urea nitrogen are seen in multiple myeloma; the renal involvement isn’t present for SPB. High levels of monoclonal protein in serum and urine are indicative of multiple myeloma. The SPB typically has absent or low serum or urinary levels of monoclonal protein…
Making ATP from Free fatty Acids:- The other two energy substrates, FFAs and AAs, bypass glycolysis and ultimately enter the TCA cycle/oxidative phosphorylation as pyruvate, acetyl CoA, or different components of the TCA cycle. FFAs are released from adipose tissue by lipolysis and circulate in blood bound to serum albumin. Transport proteins then translocate FFAs into cells. FFAs are metabolized in mitochondria by the repetitive, cyclic process of β oxidation. This requires the transport of…
Cell Transport and Permeability: Computer stimulation Jeffery Kirkland A&P Lab Courtney Charba April 17, 2015 Academic Integrity Statement: I affirm that this assignment reflects my own honest work and was completed with integrity according to the course’s academic integrity guidelines. This report is based on my own work and any ideas or words that are not my own are clearly indicated and their sources are correctly cited. Student Signature:…
Our indole test came back negative, meaning K. pneumoniae does not produce tryptophanase and does not break down tryptophan into indole, ammonia, and pyruvic acid. The urea test was performed to determine if urea is hydrolyzed via the enzyme urease. The urea test was positive, meaning K. pneumoniae produces urease to break down urea. An inoculation onto Kligler’s iron agar determines if an organism can ferment glucose and lactose, it also detects the production of hydrogen sulfide from the…
The liver which is the largest organ in the body produces bile and stores glucose. Likewise, the pancreas produces pancreatic juices which contain sodium bicarbonate (NaHCO3) and digestive enzymes for all types of food. The gallbladder, on the other hand, is a storage for bile that is produced by the liver. This information can be found on pages 649-651. These answers are correct because the bile produced by the liver emulsifies fat and blood glucose remains constant. Nonetheless, when the bile…