Urea

utilized for this test and stabbed with inoculated bacteria. A positive result would be a black precipitate at the bottom of the medium. 5. Another test used was the urea hydrolysis test. This test utilized the enzyme urease, which some bacteria produce. This enzyme breaks urea into ammonia and carbon dioxide. A urea broth tube containing a phenol red indicator is inoculated with the bacteria. When ammonia accumulates in the tube, the environment inside the tube becomes very alkaline.…

While the binding affinity increased in urea catechol complex, cysteine catechol complex and citric acid catechol complex when compared with their individual binding energies of -3.99 kJ mol-1, -0.87 kJ mol-1 and -1.27 kJ mol-1 respectively indicating lesser inhibition effect. The results are…

reagent layer on top of the broth. A positive outcome is the appearance of a red ring in the reagent layer on top of the broth. Urea Hydrolysis: Materials: - 1 Urea broth - Unknown Organism #40 - Inoculating tool Procedure: Urea broth contains phenol red a ph. indicator, urea and yeast extract to detect the hydrolysis of urea. Unknown organism #40 was inoculated into the Urea broth. Inoculating tool was sterilized with heat before and after inoculation. The inoculated broth was incubated at 37℃…

Dr John Frederick Joseph Cade Cade was born on January 18th 1912 in Murtoa, in the Wimmera region of Victoria, Australia. John Cade’s father, David served as the physician for the town of Murtoa, but on returning from his service in WWI, he sold his practice and assumed a position in the Mental Hygiene Department. Over the following years David Cade was assigned the role of medical superintendent at a number of Victorian mental hospitals, including Sunbury, Mont Park and Beechworth. John…

Urea is an extremely harmful chemical fertiliser for soil and its increased use pollutes the soil while making it unfertile in the long run. Deforestation of the upper surface of the soil in mountainous districts is increasing rapidly which compels the farmers to increase the use of urea. Due to this, soil health is deteriorating further. The unbridled use of manure, coupled with the use of pesticides…

and it includes testing urea, creatinine, sodium, and potassium.…

Indole. With the negative Indole test result this eliminates E. coli and P. vulgaris. The last test I did was use a Urea broth test for #29. The results from this test were negative which means there was no production of urease to break down urea and produce a pH change to trigger the phenol red to turn pink. This outcome eliminated Klebsiella pneumonia as a possibility. The Urea test indication concluded that the Unknown #29 was Enterobacter aerogenes. But for good measure as a Final test I…

BI374 – Phys. App. Of Microbiology TA: Aaron Coristine Student Name: Shane Thorn Student ID: 161668950 January 30, 2017 Discussion Questions General 1. Ammonification is the microbial degradation of nitrogenous compounds resulting in ammonia. Nitrification is a process by which ammonia is oxidized to nitrite and then nitrite is oxidized to nitrate. Denitrification is a process by which nitrate is converted into nitrogen gas or nitrous oxide by denitrifiers. Nitrogen fixation is a process by…

Macro and micronutrient play a vital role in the growth and productivity of fruit trees. A well-nourished fruit tree not only produces good yield but also improves quality and remains for longer period in healthy and productive condition. The literature pertaining to the problem entitled “Studies on application of macro and micronutrients in guava cv. Hisar Safeda” has been presented in this chapter. The available literature having relevance to present study has been reviwed in this chapter…

luteus. Additionally on day one, I inoculated bacteria 7B onto a Urea tube of media in order to further eliminate incorrect bacteria. The Urea tube would distinguish if bacterium 7B would produce Urease, resulting in a positive test result. After 48 hours of incubation, the result of the Urea agar slant was positive. This result reduced my possible bacterium’s identity to bacteria that would produce a positive result in a Urea agar slant. The possible bacteria were P. vulgaris, C. freundii,…