Petri dish

Aim 2: How does folliculin detect amino acids to regulate mTORC1 activity? Here, the goals are to elucidate the mechanism of action that enables folliculin to function as an amino acid sensor. 2.1. Determine the amino acid reservoir folliculin senses. Evidence points FLCN as a novel amino acid sensor. To resolve the biochemical mechanism FLCN employs, we need identify the specific amino acid reservoir FLCN senses. Lysosomes’ are the main amino acid reservoir and the ‘inside-out’ mechanism is a…

animal testing aren’t always safe for humans. A 1950’s sleeping pill caused 10,000 babies to be born with deformities. These harmful tests are not necessary anymore since there are new alternate testing methods out there. Studying cell cultures in a petri dish can produce more relevant results because human cells can be used. There are anatomic, metabolic and cellular differences between us and animals, thus the product may not always produce the same results with humans. Thomas Harting,…

Cloning is the process of creating similar genetically identical cell, tissue or individual organisms such as insects, plants or bacteria in a lab that would otherwise naturally occur in nature. Cloning is a science that has been around since before Dolly the sheep was created in a lab in the 1997, a sea urchin was cloned in 1894, it ties into many different aspects in life and is soon to be a very common thing in society. Since, and even before, Dolly the sheep scientists have been able to…

Although one may argue animals should not be used in biomedical research because there are many alternative out there to substitute animal testing, and animals has feeling and show empathy when another is hurt; however, the truth of the matter is humankinds need to use animals for biomedical testing in order to advance human race because animal testing will help us further understand human diseases, animal share many similar genetic similarity between the human, and it is unethical to use human…

I became more fascinated with in vitro fertilization after watching the program Little Women L.A. A dwarf couple was wanting to have a child of their own but was having trouble conceiving the natural way so they looked into IVF. After watching the program, I started wondering if two of my friends who want to have a child would benefit from this kind of procedure. After doing some research I discovered there are pros and cons to the procedure. Also, there are a few questions that I do not know…

who have abortions are unmarried(3). This is very high in understanding why women follow through with abortions. Pro-choice offers an alternative to the individual circumstances that the mothers may need. High poverty rates and low income create a petri dish for crime. Some may believe the mothers do not deserve the entirety of this due to the conflicting results of fathers leaving their children. Feminists feel empowered when they can control their bodies as well as their children…

Since animals testing is cruel, unreliable, and expensive, there are proven substitute methods. In vitro testing is the main alternative to vivisection. Cell cultures can be studied in a petri dish and have proven to be more effective because the cells that are used are human cells. The human cells are grown in a highly advanced laboratory to closely replicate the the structure and function of the human body and its organs. The cells are placed…

A colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich Chemical Co.) assay was performed to measure the cell viability. Briefly, RAW 264.7 cells were treated with different concentrations of spermidine for 24 h or pretreated with spermidine for 1 h before stimulation with LPS for 24 h. After incubation, the medium was discarded, and MTT solution (5 mg/mL in phosphate-buffered saline, PBS) was added to each well and incubated for another 3 h at 37 °C. The…

Eugenics is a type of genetic engineering that was intended for the improvement of the human population through artificial selection and gene editing technologies to increase the frequency of advantageous or desirable characteristics. The idea of eugenics to improve human conditions, in the form of embryo manipulation, has been debated in many scientific and cultural communities for various reasons. One of them being its effect on society, specifically the societal views of genetically improved…

What makes an orphaned child search for their parents, twins yearn for individuality, or humans desire an identity? Humans desire to have meaning in their lives by constantly looking for ways to individualize themselves. They want to be deemed significant and inimitable by the rest of the world. Kazuo Ishiguro, author of Never Let Me Go, was born and raised in Japan, until his family moved to England in 1960 when he was five years old. Throughout childhood, he is left as a social outcast,…