HeLa

Objectives Objective 1: Develop a cell line expressing the duck viperin. In order to develop an avian model that is able to express the duck viperin with an advantage of stable and homogeneous expression, duck viperin with and without the C-terminal V5 tag was previously cloned into the mammalian expression vector pcDNA3.1/Hygro+. Then, with the use of lipofectamine 2000, I stably transfected an empty vector and duck viperin tagged or untagged into DF-1 cells. In order to get a monoclonal…

way in getting to know more about her. She took it upon herself to begin the process of discovering more about her mother and in doing so, she became sick. During her investigation, Deborah slowly began to search for more and more information on the “HeLa” cells that were taken from her mother. The information that she discovered caused her extreme stress, which resulted in hives etc.”’But I always have thought it was strange, if our mother cells done so much for medicine, how come her family…

to them without any negative consequences. Had the information been given to the Lacks family from the start, many of the problems that arose could have been avoided entirely. Another way their level of education was used against them was when the HeLa cells began to be available worldwide. The Lacks family did not understand what was happening, and they believed that Henrietta herself was still alive. Neither the scientific community nor the media chose to inform them of the truth, and they got…

Both the readings bring up an important issue that plagues science and medicine. Henrietta Slacks played in important role in scientific research; her cells allowed scientists to study and come up with treatments for a wide range of diseases. HeLa cells were used to study the effects of radiation on human cells. While the cells were used for important research, one cannot forget that the original samples of cells were taken from Slacks without her consent. Her family was upset when they…

The scope of medicine is always changing. The HeLa cell line demonstrated that as it broke ground for a whole new concept of DNA that was previously unknown. Using this material for one thing, which the patient agreed to, can sometimes accidently result in a completely different discovery, one that…

Cancer is a most ravaging disease threatening globally with a leading mortally rate in developed countries. 1 Various types of cancers have been identified which affects different organs and they are broadly grouped under the category of carcinoma, sarcoma, lymphoma or leukemia based on the type of tissue origin. common striking features of all types of cancer is uncontrolled cell proliferation, antiapoptosis, enhanced angiogenesis, genomic instability and metastasis. 4 The broad range of…

Brown University researchers studying the biology of aging demonstrated a new strategy for stimulating autophagy - the process by which cells rebuild themselves by recycling their own worn-out parts. The process of autophagy involves rounding up misfolded proteins and obsolete organelles within a cell into vesicles called autophagosomes. The autophagosomes then fuse with a lysosome, which breaks down the cellular macromolecules and converts them into components the cell can re-use. Louis…

Then, when Victor Mckusick had the Lacks family give blood in 1974, it was not explained in an understandable manner to the family that the blood was being used for genetic examination to be compared to HeLa cells (Schloot, 2010). This failure in communication left Deborah Henriette’s daughter anxiously awaiting what she thought were test results to determine if she had cancer too, results that never came (Schloot, 2010). Mckusick also provided Deborah…

One was in the case of Henrietta Lacks, whose legacy lives on through HeLa cells. These cells are still around even though Lacks died over fifty years ago (Josefson). The scary part of this story is that an inconceivable amount of money has been made from HeLa cells, yet the Lack's family has never seen any profits from them (Josefson). Showing just how corrupt the United States health care system has become, since…

Anna Sanders 9th Grade Biology The Immortal Life of Henrietta Lacks Introduction “The Immortal Life of Henrietta Lacks” by Rebecca Skloot tells the story of Henrietta Lacks and her cells (the HeLa cells). Henrietta was the mother to five children. She was the wife to David ("Day") Lacks, who was also her first cousin. She was diagnosed with cancer when she was thirty years old. She died when she was thirty-one years old. Henrietta never told her family about the “lump” she had until she…