C-terminus

Objectives Objective 1: Develop a cell line expressing the duck viperin. In order to develop an avian model that is able to express the duck viperin with an advantage of stable and homogeneous expression, duck viperin with and without the C-terminal V5 tag was previously cloned into the mammalian expression vector pcDNA3.1/Hygro+. Then, with the use of lipofectamine 2000, I stably transfected an empty vector and duck viperin tagged or untagged into DF-1 cells. In order to get a monoclonal stable cell line, I plated on 96 well plates for isolation and separation of a single clones. To confirm the transfection, a western blot technique was used to confirm the expression of the tagged viperin in DF-1 cells; a band size with 42 kDa should be detected.…

The full name of EHBP1 is "EH domain binding protein 1." The symbol for the gene is EHBP1. Other names for the EHBP1 gene are HPC12, NASCIN, and KIAA0903. EH Domain Binding Protein 1 is a protein coding gene. This gene codes for an Eps15 homology domain binding protein. Its protein type is a vesicle. The encoded protein may play a role in endocytic trafficking. It is associated with prostate cancer. This gene is commonly found within the cytosol and the plasma membrane of a cell. It's size is…

Sequence one’s protein was found to be apolipoprotein E. This protein is known as the APOE gene. The protein forms molecules called lipoproteins by combining fats in the body. Lipoproteins have the responsibility to package cholesterol and other fats, and send them through the bloodstream. To prevent disorders with this protein, it is important to maintain the right level of cholesterol, so that it does not affect the heart and blood vessels that could causes a heart attack, stroke, or a…

What impact has TM Landry had on me? An impact is the way an outside force influences an individual’s behavior. Everyone has experienced an event that has shaped their beliefs or influenced their actions. These events can vary from a death to moving schools. This was the case with Sean. Sean waited at the bus stop as he replayed the last two weeks. Sean’s dad had recently moved them to a different state for his new job. Since Sean was an introvert, he dreaded the idea of starting over in…

The protein that is in sequence one is Alpha-synuclein. Alpha-synuclein is a protein whose function in the healthy brain is currently unknown (Alpha-synuclein and Parkinson's Disease, n.d.). Parkinson's disease researchers take great interest in it because it is a major constituent of Lewy bodies, which are protein clumps that are the pathological hallmark of Parkinson’s disease (Alpha-synuclein and Parkinson's Disease, n.d.). Researchers work to characterize the protein's role in Parkinson’s…

I had got use to having the skytrain doors shut in my face. No matter how confused or scared someone looked, no one had the time to stop or help a lost person. The ride back home seemed to be longer than usual and I could not help but notice the nervous group of kids trying to figure out the directions. Looking at them reminded me of the time when I first used the skytrain without any adult supervision. I vividly remember the struggle my little cousin and I faced just to get back home. It…

Colorado a place where there are beautiful snowy mountains and a bunch of scenery. A girl from Los Angeles, California that would be the weirdest thing to imagine for someone who grew up around beaches and warm weather all the time. That girl was me, I just learned that I was moving from sunny California to bi-polar weather Colorado. Learning that I was moving to a completely different state was one of the hardest and weirdest things for me. During the months of September and October my…

WHAT IS OBJECT ORIENTED PROGRAMMING? Object oriented programming in a methodology of software development that allows for the designation of functions to be applied to data structures such as classes, these classes are referred to as objects, hence the name. Object-Oriented coding is one of multiple major programming paradigms. These include but are not limited to; Imperative, Logical, Functional and Object oriented. Problems that can be solved by one of these paradigms can normally be solved by…

There are different types of programming such as object-oriented programming (OOP), event-driving programming (EDP), and procedural program, (PP). Some people may agree that there are advantages of using OOP instead of only using PP. A class may have one attribute and one method, in which the class represents an event, the attributes the class stores and the purpose of the method. The class, attributes, and methods share a relationship. Visual Logic is used to learn about programming but lacks…

When I started this course, I was unaware of the different types of writing and the massive amounts of research behind it. Prompts given to me were thought provoking and interesting, so I took a lot of interest in various types of arguments and assignments. What caught my interest in this subject is how, as a writer, one has to argue from both sides, include research data to support them, and then conclude them with a winning argument. My collection of persuasive writing demonstrates that I…