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  • Trypan Blue Exclusion Lab Report

    This active process would only occur in a living organism since requires energy, in the form of ATP. This process requires reducing agents, NADPH in the cytosol and NADH from the nucleus, to reduce into red coloured products and cause alterations in metabolic activity. Iodonitrotetrazolium reduction would most likely be observed in the later stages of toxicity since it requires energy. What effects would you expect to see from menadione exposure if the hepatocytes were preincubated with: ascorbic acid (Vitamin C), buthionine sulfoximine (BSO, an inhibitor of glutathione synthesis), trolox (tocopherol analogue) Exposure to menadione (Vitamin K3) will lead to the generation of reactive oxygen species and free radicals produced from redox cycling. High concentrations of reactive oxygen species can result in damage to tissues, mitochondrial DNA damage as well as cell death by necrosis or apoptosis (Loor et al., 2010). Glutathione (GSH) is essential in combating oxidative stress, due to the sulfhydryl group present on the cysteine residue. However, the levels of GSH are depleted in the presence of the reactive oxygen species produced by…

    Words: 1419 - Pages: 6
  • COPD Case Study

    the disulphide bridges of Glycoproteins of the bronchial mucous and when Cysteine donator. It is postulated that the resulting reduction in viscosity oral removal of the bronchial facilitates mucus,were by mucociliary clearance is increased. Furthermore. NAC possesses antioxidant properties, because it acts on the one hand directly Oxidantienabbauend by its free thiol group can be oxidized. This electron acceptor function, consequently leads to a reduction of reactive Oxygen species such as Lien…

    Words: 945 - Pages: 4
  • Measurement Of Glutathione Essay

    Measurement of glutathione Glutathione (GSH) is a ubiquitous tripeptide which serves several vital functions including detoxifying electrophiles, scavenging free radicals and providing a reservoir for cysteine. The sulfhydryl group of GSH reduces 5-5'-dithiobis (2-nitrobenzoic acid) (DTNB) and form the yellow colour product 5-thio-2-nitrobenzoic acid (TNB)21. Measurement of the absorbance of TNB at 412 nm provides an accurate estimation of GSH in the sample. Figure 7. Materials 5×105 cells/mL…

    Words: 1062 - Pages: 5
  • Glutathione Peroxidase Lab Report

    activity in the gastric tissue was measured according to the procedures of Sinha (1972). One ml of homogenate solution was taken and mixed with 5 ml of phosphate buffer of pH 7.4. To this 4 ml of 0.2 M H2O2 was added and time noted. Exactly after 180 seconds of adding H2O2, a set of 1ml of reaction mixture from the above was taken and 2 ml dichromate acetic acid was added. It was then kept in a boiling water bath for 10 minutes. All the tubes were then cooled under running tap water and finally…

    Words: 801 - Pages: 4
  • Polymerase Chain Analysis

    Objectives: To evaluate the association of glutathione S- transferase mu 1 (GST M1) and glutathione S- transferase theta 1 (GST T1) polymorphisms with the development of T1DM and disease-related risk factors. Materials and Methods: The study included 64 diabetic children with T1DM and 41 control subjects. They were subjected to: history taking anthropometric measurements and laboratory parameters included fasting glucose, serum creatinine, lipid profile, HbA1cand evaluation of GST T1 and M1…

    Words: 1684 - Pages: 7
  • Detox At Home Analysis

    the liver function and also get rid of all the toxins from the body. It helps to increase the detoxification enzymes and cleans the system. 3. Cabbage – Cabbage is known for its diuretic properties. It takes a lot of calories to chew on cabbage. Cabbage is primarily composed of water and it helps to flush out the toxins from the body. Cabbage, like most raw vegetables, contains glutathione, which aids the liver in the process of detoxification. Cabbage also has high nutrient value. It supplies…

    Words: 427 - Pages: 2
  • Avocado Research Paper

    one of the healthiest foods on earth. They consist of 25 essential nutrients that include potassium, iron, phosphorus, copper, magnesium, vitamins A, C, K, E and K. Avocados are also found to possess certain useful phytochemicals like glutathione, beta-sitosterol and lutein as well as fiber protein. This article focuses on the health benefits of avocados. Reduces cholesterol levels Avocados are rich source of beta-sitosterol compound which is found to be effective in lowering the cholesterol…

    Words: 630 - Pages: 3
  • Physics Of Q-Dots Lab Report

    frequency and emits color as well as large size which has a low frequency. Therefore, Q-Dots size have been extensively studied in the past years because of their physical, chemical, and electronic properties change due to quantum confinement effects. Many different semiconductor materials can be used to create Q-Dots, such as Cd/Se, Cd/S, In/P and In/As. Also, there are many studies that reported that preparation Glutathione-capped CdS, CdSe and CdTe nanoparticles.2 There are a few different…

    Words: 2135 - Pages: 9
  • Summary Of The Effect Of Honokiol On APP-CHO Cells

    Increased oxidative stress is directly related to depletion of GSH [18]. Apoptosis due to ROS generation is significantly related to diminished GSH content [19]. Therefore, we sought to estimate the effect of Honokiol on the expression of GSH levels in APP-CHO cells using Glutathione Reductase Activity assay. High insulin exposure significantly reduced GSH content (72%) as compared to the control. Honokiol improved GSH content at both 5 and 10 μM doses, with statistically significant effect at…

    Words: 1609 - Pages: 7
  • Essay On The Difference Between Hydrochloric Acid And Tbars

    It was given to all groups except the normal one. Cranberry extract and vitamin C were orally given daily for 3 weeks and the last dose of each was given 1 h before Diclofenac sodium administration. Blood samples were collected 24 h after Diclofenac sodium administration. Values are given as mean ± SD for groups of eight animals each. * Significantly different from normal group at p< 0.01 Table 2: Level of blood reduced glutathione (GSH) and activities of superoxide dismutase (SOD) and…

    Words: 1581 - Pages: 7
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