Southern blot

Article Comparisons to Southern (1975) Article One The article “Detection of specific sequences among DNA fragments separated by gel electrophoresis” (1975) by E.M. Southern focuses on the way that the fragments of DNA can be transferred from agarose based gels to cellulose nitrate filters. Then fragments themselves are hybridized to active RNA. E.M. Southern’s main influences for the investigation were the studies of Smith and Wilcox (1970), and Kelly and Smith (1970), which showed that the restrictive enzyme Haemophilus influenza was able to make breaks in DNA sequences that were double stranded. The fragments that were made by the enzymes could be separated by electrophoresis in agarose gels, which would elucidate a picture of the fragments…

This book tells the story of Damon Courtenay as he tries to overcome the adversities caused by haemophilia and Aids. After I finished this book, I was disappointed by the treatment people with HIV and Aids received, particularly by the men and women in the medical profession. I don't want to sound hypocritical and judge these people because personally, I don't know how I will react to such situation, particularly in the 1980’s where the knowledge of this disease was very low. I also believe…

Overall, there would be four Western blots completed. There would be two experimental Western blots completed: blot one would contain the ER fraction and blot two would contain the transmembrane fraction. Both blot one and two would be exposed to primary and secondary antibodies (one primary Ab would be specific for MH1 and one primary Ab would be specific for MH2). There would be two control Western blots completed: blot three would contain the ER fraction and blot four would contain the…

Objectives Objective 1: Develop a cell line expressing the duck viperin. In order to develop an avian model that is able to express the duck viperin with an advantage of stable and homogeneous expression, duck viperin with and without the C-terminal V5 tag was previously cloned into the mammalian expression vector pcDNA3.1/Hygro+. Then, with the use of lipofectamine 2000, I stably transfected an empty vector and duck viperin tagged or untagged into DF-1 cells. In order to get a monoclonal…

The nitrocellulose piece was first submerged in nanopure water. Then, the blot sandwich was assembled in a tray of transfer buffer. One side of the transfer clamp was placed on the bottom of the tray and filled with transfer buffer. One Scotchbrite pad was submerged onto the bottom of the transfer tray. A piece of blotting paper was submerged onto the pad. The gel was then removed from the cassette and the top left corner was clipped. The gel was laid on top of blotting paper with the clipped…

The slave holding in the South increasingly felt its interests were threatened, particularly since slavery had been prohibited in much of the new territory that had been added west of the Mississippi River. The arguments for the right of secession are indisputable. The constitutional right based on the Compact Theory, and the revolutionary right based on the idea that a free people have the right to change their government anytime they see fit, in which they did. Two phases of antislavery…

However, because Hall was only prosecuted, mixed signals were sent about the stance on lynchings. Claude A. Clegg III writes, “It simultaneously signaled that lynchings were becoming unacceptable expressions of extralegal retribution and confirmed that local and state authorities were limited in their willingness to pursue lynch mobs.” Hall’s prosecution brought to light a union of political and cultural trends that had distinguished southern history since the slave emancipation in 1865. As a…

Harper Lee was born in Alabama in 1926. She was the youngest of four and considered herself a tomboy (similar to Scout). Her mother suffered from a mental illness and rarely left the house (similar to Boo Radley). Lee studied law (similar to atticus) and then published To Kill a Mockingbird in 1960 during the Civil Rights Movement. Her novel went on to win the Pulitzer Prize a year later. In her novel, To Kill a Mockingbird, the social and gender inequalities are the main issues. Lee approaches…

The novel, “To Kill a Mockingbird,” was written from a child’s point of view at the beginning of the Civil Rights Movement. Harper Lee used actual event in her life to fabricate the foundation of the novel. It expressed the views of racism concerning justices with a gothic mixed in the context. The novel was centered on a child seeing everything in black and white. Lee used characters to symbolize mockingbirds. The novel experienced character persona of good and evil. There were similarities…

Scout’s tom boyishness is depicted in the beginning of the novel and draws attention to how her feminist attributes add on. Sometimes people may force Atticus to see the “bigger picture” of the traditional southern women stereotypes. Scout does not agree with the idea of Alexandria trying to turn her into a traditional Southern woman (73). “Since the mother of Scout and Jem is dead, Atticus has assumed the full responsibility of playing mother and father whenever possible – though admittedly he…