Recombinant DNA

only one way based on Chargaff’s rule. b) Franklin’s x-ray diffraction photographs showed that the wet form of DNA likely had a double helix. Wilkins showed Watson Franklin’s results and at the time Watson and Crick were experimenting with many potential models of having 1,2 or even 2 strands. As soon as Watson saw the double helix, he knew the double strand model was correct. 29. DNA contains phosphorus but very little sulfur and protein contains sulfur but very little phosphorus. Hershey…

save lives through early detection. There was also a need to better and more reliably analyse DNA from crime scenes and use this information to find and detain suspects. Through composing a detailed portfolio of an individual’s DNA there would be no question in determining if the suspect was present at the crime scene. Function – This innovation served the function of locating similarities in the DNA of relatives to help in early detection of genetic diseases. It also helps to…

The psbC sample was loaded along with a DNA ladder to assure that the sample was within the range of the predicted size. During the PCR amplification the region in the synechocystis that gets amplified is between 662 to 2,819 bp which makes the target gene approximately 2,157 bp. We used primers…

inorganic and poses risks to human health. Genetically modified products are synthesized in laboratories with toxic chemicals, and it adversely affects the human genome. Genetic modification uses recombinant technology extensively, in which DNAs from two different species is joined together. Through recombinant technology, genetic engineers infuse desirable traits into organisms…

One massively noted benefit is the production of a new breed of drought resistant corn. (Paarlburg) These new breeds, whether created through selective breeding or recombinant DNA technology, are able to be used in the more arid areas such as Africa or many Middle Eastern countries. These areas often suffer due to their inability to produce food at a high rate, which increases the starvation rates in their countries. This…

CRISPR Genes – A way to eradicate Autism to Cancer to Zellweger syndrome and everything in-between It was September 20th 2001. It was the day my mother would be screened if the fetus (i.e. me in my glorious self) showed an elevated risk for Downs Syndrome. A triple screen blood was performed which measured the quantities of three substances: Alpha-fetoprotein (AFP) which is produced by the fetus, human chorionic gonadotropin (hCG), and unconjugated estriol which is produced by the placenta. The…

growing population and a limited food supply. GMOs are defined as an organism whose genome has been altered by the techniques of genetic engineering so that its DNA contains one or more genes not normally found there. This is typically accomplished through transgenesis, which is the usage of a bacterium to transfer DNA. Humans Altering DNA began, as Charles Darwin described, with selective breeding, or artificial selection. Humans choose which organism to breed and, hence, choose with genes to…

different color mouse (agouti color) and induces the specific genetic mutation via synthetically modified DNA. This DNA includes components required to test the embryonic stem cells via positive and negative selection for the desired mutation. Positive selection involves the use of G418 (antibiotic), whereas negative selection involves the use of ganciclovir (drug that combats viruses). The DNA inserted into the embryonic stem cells was composed of two indicator genes: neor, which is resistant…

ability to conduct DNA analysis revealing varying compositions and properties. For the following experiment the genomic DNA from Drosophila melanogaster, was utilized to be cloned and sequenced for either actin or 18s rRNA. In order to clone and sequence the specific section of DNA 9 specific steps had to be conducted and they are as follows: extraction/purification of DNA, amplify the gene utilizing PCR, ligate the product from PCR into plasmid vector, use E. coli to transform plasmid DNA,…

In this context, the development of recombinant DNA technology in the 1970s marked the beginning of a new era in biology (Jackson et al. 1972). This beginning promoted a new born in genetic engineering boosting the modern biotechnology and other science fields. Thanks to this discover, scientists…