Analysis Of Genomic DNA From Drosophila Melanogaster

The study of inheritance at the at one of the smallest levels is referred to as molecular genetics and is utilized for many purposes, which include genes, genotyping, sequencing, cloning, etc. (Kelly, 2016). This allows for the ability to conduct DNA analysis revealing varying compositions and properties. For the following experiment the genomic DNA from Drosophila melanogaster, was utilized to be cloned and sequenced for either actin or 18s rRNA. In order to clone and sequence the specific section of DNA 9 specific steps had to be conducted and they are as follows: extraction/purification of DNA, amplify the gene utilizing PCR, ligate the product from PCR into plasmid vector, use E. coli to transform plasmid DNA, purify recombinant plasmid DNA, quantify plasmid DNA, conduct restriction analysis of plasmid DNA, conduct DNA sequencing, and lastly analyzed DNA sequence (Garey, Moore, Yoder, Harmon, & Mason, 2016). To begin, a DNA extraction was conducted for Drosophila melanogaster using the method of lysis, which is when the membrane of the cell is compromised and the contents within are released (Brooker, 2009). For the purpose of this experiment sodium dodecyl …show more content…
For experiment 6 the solution from the previous experiment is needed, varying tubes, master mix, ice, and a PCR machine. For experiment 7 varying tubes are needed, the product from the previous lab, pipette, ice, heat block, SOC, and incubator. For experiment 8 a agarose gel is needed along with ice, pipette, toothpick, centrifuge, vortex, buffer P1, buffer P2, buffer N3, buffer PE, and sterile water. For experiment 9 a NanoDrop machine is needed, a pipette, buffer, and incubator. Lastly for experiment 10 the plasmid DNA from the previous experiment is required along with a pipette; a primer provided by the TA, and a master mix (Garey et al.,