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100 Cards in this Set

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1 - The main energy source for most living cells.
ATP - adenosine triphosphate.
2 - These are the primary compounds that make up cell membranes.
Phospholipids and proteins??
Gram + and - :Peptidoglycan
Gram - : lipopolysaccharides, lipoproteins.
3 - Composed of glycerol and fatty acids.
Lipids (Fats)
4 - "Genetic" material of cells.
DNA or RNA but never both.
Nucleotides: sugar, phosphate, and nitrogen held together by a hydrogen bond.
Keyword=genetic; what is genetic in a cell?
5 - Antibodies and enzymes are examples of ____.
Proteins.
6 - The most important solvent in living cells is ____.
Water.
Hydrogen.
7 - Insoluble in water; Sterioids are examples of ___.
Fatty acids: Lipids.
8 - DescribeD the structure of DNA.
Watson and Crick
9 - The major proponent of spontaneous generation.
John Needham insisted in spontaneous generation.
Scientific community observed flies on meat. Pasteur proved otherwise.
10 - Father of microbiology; disproved spontaneous generation.
Louis Pasteur
11 - Discovered penicillin.
Flems Pens.
Flemming.
12 - Discovered phagocytic cells.
Mechnikov.
13 - Developed the vaccination for smallpox.
Jenner.
14 - Responsible for the one enzyme/one protein theory.
Beadle and Tatum.
15 - First to associate a specific organism with a specific disease.
Koch.
16 - First to observe microorganisms.
van Leeuwenhoek=microorganism.

however, Hooke first observed cells.
17 - First to use an antiseptic/disinfectant; carbolic acid.
Lister.
18 - Viruses that infect bacteria are called ___.
Bacteriophage.
19 - This type of gene transfer can transfer the most genetic material.
Bacterial Conjugation..
20 - DNA to mRNA is what?
Transcription.
21 - Genetic transfer involving "Naked DNA".
Transduction is the transfer of DNA from one cell to another.
Nucleic acid?
22 - This type of enzymes are always present in the cell.
Constituitive
23 - Jumping Genes.
Transposons
24 - These enzymes are only present if the substrate is present.
Inducible
I've deduced that the substrate is needed for the enzyme to be present. If she's there I'll be there.
25 - This is a base substitution mutation.
Point or base substitution:
Missense, nonsense, Silent.
26 - These organisms are classified by locomotion.
Protozoa.
27 - Submicroscopic intracellular parasites.
Viruses are submicroscopic intracellular parasites that consist of either RNA or DNA (deoxyribonucleic acid)—never both.
Smaller than bacteria and filterable.
28 - These organisms reproduce by spores.
Fungi.
29 - These divide by binary fission.
Bacteria.
30 - These microbes have no cell wall, only a cell membrane.
Protozoa have no cell walls and viruses are not considered cells so have no cell walls .
31 - This causative agent of CJD is an infectious piece of "protein".
Bovine disease
CJ wears bo-bo's and climbs vines.
32 - Infectious pieces of RNA that can cause some plant diseases.
Prions
33 - These organisms have a cell wall composed of Chitin.
fungi.
34 - These microbes are photosynthetic.
Algae and cyanobacteria.
35 - These microbes possess only on chromosome.
Prokaryotes.
36 - This virus is the most common cause of pneumonia in infants.
RSV
37 - Breakbone fever.
Dengue fever.
38 - This virus results in a slapped cheek appearance in children.
Fifth disease.
39 - This virus is often associated with cervical cancer.
Genital warts - papollomis.
HPV
Human Popllomis virus
40 - One must have chickenpox first to develop this infection.
Shingles.
41 - This virus is spread by inhalation of dried urine from infected rodents.
Hantavirus pulmonary syndrome
hint=soda, Fanta orange, Urine look.
Hand full of dried rodent urine.
42 - Coldsores.
Herpes simplex.
Permanent houseguests.
43 - Vaccinations for this infection include Salk, Sabin, and OPV.
Polio
44 - Koplik spots are characteristic lesions of this infection.
Measles (Rubeola).
45 - This disease has been completely eradicated by vaccination in 1980.
smallpox.
46 - Negri bodies are characterisitic inclusion bodies of this virus.
Rabies.
47 - A salivary gland infection that can lead to sterility in postpubertal males.
Mumps.
48 - 121 C at 15 psi for 15 mins.
Thermal death-point.
Autoclave. To kill even endospores. Every 15 min. 90% are killed. It is referred to as Thermal death-point.
49 - Another term for "cleaning".
Sanitization.
50 - 100 C.
Pasteurized milk is brought to 100 degrees and can keep for a long periods of time without refridgeration if seal has not been broken.
Autoclave set to 100c will increase to 121c when adding 15 PSI.
boiling water.
51 - 62c for 30 minutes.
Pasteurized Milk.
52 - This germicidal activity is about 260 nm.
UV light is 260nm
53 - Freeze-drying to preserve bacteria.
Lipholization.
54 - During this growth phase cells are metabolically active; preparing to divide.
Lag phase:
Intense activity preparing for population growth but no increase yet.
55 - During this growth phase cell death is equal to cell multiplication.
Stationary phase:
Period of equilibrium; microbial deaths balance poduction of new cells.
56 - Cyanobacteria are examples of this nutritional type.
Photoautotrophs.
57 - During this growth phase cells divide exponentially.
Log phase:
Logarithmic or exponential, increase in population.
58 - Nitrifying bacteria are examples of this nutritional type.
Chemoautotrophs
59 - Most pathogens and normal microbiota are classified in this group.
chemoheterotroph
60 - This organism is an acidophile; causes gastric ulcers.
Helicobacter pylori.
61 - A gram negative bacillus; normal microbiota in the large intestine.
E. Coli
62 - These are gram positive cocci in clusters.
Staphylococcus aureus????
Nosocomial infections?
34% of nosocomial infections stem from Gram positive cocci.
63 - The aerobic sporeformer genus.
Genus: Bacillus
64 - The genus of anaerobic sporeformers.
Clostridium?
(pretty sure this is correct)
65 - Baker's yeast; produces alcohol by fermentation.
saccharomyces cerevisiae
66 - All members of this genus are acid-fast.
Mycobacterium.
67 - This is an example of an obligate halophile.
halobacterium salinarium??
Requires salt up to 30%.
(this is ONE example or is it the genus?)
68 - The yogurt bacteria.
A probiotic = Lactobacillus acidophilus
69 - This genus has no cell wall; a naturally occurring protoplast.
Protozoa??... Genus: protist;
Mycoplasma is a genus of bacteria which lack a cell wall.
70 - The doorway to the Krebs cycle.
Prepatory stage or Acetyl CoA. ?
Intermediate step/cycle
71 - Carbon dioxide + water
---> Glucose + water + oxygen.
Photosynthesis???
72 - This phase of cellular respiration takes place in the prokaryotic cell membrane.
Electron transport chain.
73 - Glucose ---> pyruvic acid
Glycolysis.
74 - An organism that cannot grow without oxygen.
Obligate Aerobe.
75 - These organisms require oxygen at a lower concerntration found in the atmosphere.
Microaerophiles.
76 - These microorganisms grow better at high temperatures.
Thermophiles.
77 - These organisms prefer oxygen but can ferment if oxygen is absent.
Falcultative Anaerobes.
78 - These are catalase negative organisms.
Anaerobes.
Everything has catalase thus when you put hydrogen peroxide on a wound it bubbles showing that catalase is present.
79 - These organisms grow in the refridgerator.
Psychrophiles
80 - these organisms grow best at room temperature or body temperature.
Mesophiles
81 - Who was Semmelweiss?
Advocated Hand washing.
82 - What is recombinant DNA?
Combining of DNA from two different sources.
83 - What is the purpose of endospores?
It's used as verification for killing steralization especially within autoclaves. The endospores area created when staple life nutrients are scarce in order to continue life.
84 - What kind of chemical bonds hold organic molecules together?
Covalent bond
85 - Which bacteria are used to test the effectiveness of disinfectants?
Salmonella cholera,
Pseudomonas aureus,
Staph. aureus.
S.P.S.
86 - What is the pH of an acid? A base?
Potential Hydrogen.
Less than 7 is an Acid.
Greater than 7 is a Base.
87 - What is a chemically defined medium?
Knowing the exact amounts and ingredients.
88 - What is ethylene oxide?
What is it used for?
Medical Sterilization - Kills endospores.
Sweet oder.
used to sterilize substances that would be damaged by high temperature techniques such as pasteurization or autoclaving.
89 - What is a capnophile?
Carbon dioxide loving.
90- Which organisms are "Permanent house guests".
cold sores = herpes simplex
Shingles = chickenpox
and normal microbiota.
91 - What is a zoonosis?
A disease that is transferable from an animal to a human.
92 - What caused the pandemic of 1918 that killed more people than WW1?
Influenza.
93 - What bacteria is used in the Ames Test?
Salmonella to detect Histones.
94 - What is the difference between competitive and noncompetitive enzymes?
Competitive bind to enzyme preventing substrate from adhering to same specified area.
Noncompetitive bind to a separate area causing the enzyme to alter it's shape so that it prevents a substrate from binding.
95 - What is monotrichous? How many flagella?
bacteria containing one flagella.
96 - What are the examples of teratogens we studied?
Cytomegalovirus
Herpes
Rubella
97 - How can you grow viruses?
1 - bacteriophages mixed in
2 - nutrient agar with
3 - host bacteria
98 - How is hepatitis A transmitted?
Through fecal/oral route.
99 - What is the most severe type of hepatitis?
Has the highest mortality rate?
Hepatitis D
severe high mortality rate
100 - Which type of bacteria has a lipoplysaccharide outer membrane.. gram + or gram -?
Gram - has the multi-layers of cells walls including the lipopolysaccharide and produce Endotoxins.