Microbiology Lab Final Study

Front 1

Normal flora or intestines; lactose fermenter; gram - rod.

Back 1

E. coli

Front 2

Acid fast; saprophyte; gram+ rod

Back 2

Mycobacterium smegmatis

Hint 2

keyword= acid fast. What are acid-fast.

Front 3

VP; positive; citrate positive; gram - rod.

Back 3

Enterobacter aerogenes.

Hint 3

Hint= ONLY VP may Enter but the orange citrus may give you diarrhea.

Front 4

Grows on mannitol "salt" agar; facultative halophile; lipase positive; most antibiotic sensitive organism used in lab; gram+ cocci.

Back 4

Staphylococcus aureus.

Front 5

Aerobic sporeformer; caseinase positive; gram+ rod.

Back 5

Bacillus subtilis.

Hint 5

hint= Casey is positively full of BS. Yeah, a real fun guy.

Front 6

Urease positive; hydrogen sulfide positive; gram- rod.

Back 6

Proteus vulgaris

Hint 6

hint= Urease and H. sulfide are vulgar.

Front 7

"Obligate halophile"; requires 15% salt.

Back 7

Halobacterium salinarium.

Front 8

Opportunistic bacteria; blue-green pigment aerobic; non-fermentative; lipase positive; oxidase positive; gram- rod.

Back 8

Pseudomonas aeruginosa.

Hint 8

hint= blue-green is psychodelic.

Front 9

Produces a "red pigment" at room temperature; gram- rod.

Back 9

Serratia marcesans.

Hint 9

hint= like a Serrated sunset.

Front 10

Citrate positive; produces alkaline results in fermentation media; gram- rod.

Back 10

Salmonella

Hint 10

hint= Salmon drink milk.Orange-Fish (citrate) swim in milk/alkaline.

Front 11

Anaerobic sporeformer; gram+ rod.

Back 11

Clostridium sporogenes.

Hint 11

hint= girls clostridium do anaerobics.

Front 12

Produces a yellow pigment.

Back 12

Micrococcus lutea.

Hint 12

hint= small yellow tea

Front 13

Phenol is used as a ___.

Back 13

control

Front 14

Yellow-acid, gas; maybe a bubble; phenol red/ Durham tube.

Back 14

Fermentation

Hint 14

Keyword= bubble therefore there's carbondioxide. Why the production of carbon dioxide?

Front 15

Red; MRVP broth; "formic" hydrogen; methyl red indicator

Back 15

Mixed acid fermentation.

Hint 15

Hint= Mr. VP ferments mixed acid and butanediol.

Front 16

Red color developes;
"Barritt's" reagent A and B;

Back 16

Vogues-Proskauer test

Hint 16

hint= mrVP

Front 17

Bubbles; hydrogen peroxide; nutient agar.

Back 17

Catalase production.

Hint 17

Keyword= catalase

Front 18

Colonies turn purple; Oxidase test reagent; oxidase; trypticase soy agar (TSA)

Back 18

Oxidase Production

Hint 18

Hint= what is it testing for? It's an Oxidase test reagent.

Front 19

Red color developes; nitrite reagents A and B;

Back 19

Nitrate reduction

Front 20

Clear areas next to growth; iodine; amylase.

Back 20

Starch Hydrolysis.

Front 21

Clear areas next to growth; no test reagents; skim milk agar.

Back 21

Casein Hydrolysis

Hint 21

Keyword=Casey may be teased by Jello but Casey loves milk.

Front 22

"Dark blue" precipitate; no test reagent; "lipase"; spirit blue agar.

Back 22

Fat Hydrolysis.

Hint 22

keyword=BLUE FAT melt.

Front 23

Fuschia color; no test reagent; urea broth.

Back 23

Urea hydrolysis.

Hint 23

Keyword = urease

Front 24

Red if positive for indole; Kovac's reagent; What is produced or occurs or what gets hydrolyzed?

Back 24

Tryptophan hydrolysis.

Hint 24

keyword= Bob Dole is trippin on Cojac.

Front 25

Agar turns black; no test reagent; triple sugar iron agar.

Back 25

Hydrogen sulfide production

Hint 25

hint= Black from the pits of hell; fire and brimstone.

Front 26

Prussian blue color change; no reagents; ___ medium.

Back 26

Citrate production

Hint 26

keyword= Prussians like blue but want a color change to citrus orange.

Front 27

Deep green color develops; 10% ferric chloride; ___ agar.

Back 27

Phenylalanine deamination

Hint 27

Keyword= Phen.
hint= color changes from color to green tint.

Front 28

growth near top of broth-aerobe; thioglycollate broth.

Back 28

Oxygen requirements

Hint 28

hint= there are aerobes therefore require ___.

Front 29

2, 3-butanediol "Fermentation"

Back 29

MR-VP broth

Hint 29

hint= Mr. VP ferments butane like wine.

Front 30

carbohydrate fermentation

Back 30

Kliger's iron agar

Hint 30

hint= ironing sugar.

Front 31

Casein hydrolysis - What's the medium?

Back 31

Medium : Skim milk agar

Hint 31

hint= Casey's medium length milky white skin.

Front 32

Citrate utilization - What's the medium?

Back 32

Medium : Simmon's citrate agar

Hint 32

hint= Simon says utilize citrate.

Front 33

What's the medium used with Hydrogen Sulfide production?

Back 33

Medium : Sims medium and Kliger's iron

Hint 33

hint= Blackened iron and sims medium.

Front 34

Mixed acid fermentation - What's the medium?

Back 34

Medium : MR-VP broth

Hint 34

hint= Mr. VP ferments mixed acid and butanediol

Front 35

Triglyceride hydrolysis - What's the "medium"?

Back 35

Medium : Spirit blue agar

Hint 35

hint= Triglyceride's medium is ___ ___ ___ .

Front 36

What is the "medium" used for Tryptophan degradation?

Back 36

Medium : Tryptone broth

Hint 36

keyword= The tone is medium.
Tip-toe through the medium is so degrading.

Front 37

What's the "reagent" for 2, 3 butaNediol fermentation?

Back 37

Alpha naphthol

Hint 37

hint= The FIRE of Alpha.
NOT the medium but it is asking for the reagent.

Front 38

What's the "reagent" for the catalase "test"?

Back 38

Hydrogen peroxide

Hint 38

hint= reagent to test for catalase.

Front 39

What's the "reagent" for "mixed" acid fermentation?

Back 39

Methyl red.

Hint 39

hint= mixed folk do Meth
Like Agent Orange it's
Methyl Red.

Front 40

What's the "reagent" for nitrate reduction?

Back 40

Sulfanilic acid.

Hint 40

hint= Licking nitrate.

Front 41

What's the "reagent" for oxidase test?

Back 41

NNNN, - tetramethyl-phenylenediamine dihydrochloride.

Hint 41

hint= reagents: like O2 there's N4.

Front 42

What's the "reagent" for pheylalanine deamination?

Back 42

Ferric chloride.

Hint 42

hint= Ferrits are deaminated.

Front 43

What's the "reagent" for starch hydrolysis?

Back 43

Gram's iodine.

Hint 43

hint= That agent is a white guy.

Front 44

What's the "reagent" for tryptophan degradation?

Back 44

Kovac's reagent

Hint 44

Cojak is so degrading.. what a trip.

Front 45

What's the "enzyme" for this reaction: Casein hydrolysis.

Back 45

Protease.

Hint 45

hint= Teasing Casey with Jello.

Front 46

What's the enzyme for this reaction: Gelatin liquifaction.

Back 46

Protease

Hint 46

hint= Teasing me with jello pudding.

Front 47

What's the "enzyme" for this reaction: hydrogen sulfide production.

Back 47

Cysteine desulfurase.

Hint 47

hint= H.S. produced the cystein chapel.

Front 48

What's the ENZYME for this reaction: indole.

Back 48

Tryptophanase

Hint 48

hint= The Bob Dole trip.

Front 49

What's the ENZYME for this reaction: starch hydrolysis.

Back 49

Amylase.

Hint 49

hint= Amy loves spagetti.

Front 50

What's the ENZYME for this reaction: Triglyceride hydrolysis.

Back 50

Lipase.

Hint 50

hint= Fat lipped enzyme

Front 51

What's the enzyme for this reaction: Urea hydrolysis.

Back 51

Urease.

Hint 51

hint = erasing urea.

Front 52

What's the product for this reaction: Catalase.

Back 52

Molecular oxygen.

Hint 52

Hint= Catalase hydrolysis hydrogen peroxide thus creating the product "oxygen".

Front 53

What's the product for this reaction: Phenylalanine deamination.

Back 53

Phenylpyruvic acid.

Front 54

What's the product for this reaction: Triglyceride hydrolysis.

Back 54

Fatty acids

Hint 54

keyword= triglyceride hydrolysis is melted fat acid.

Front 55

What's the product for the reaction Urea hydrolysis.

Back 55

Ammonia

Hint 55

Hint = ureA.

Front 56

What's the product for this reaction: Voges-Proskauer test.

Back 56

2, 3-Butanediol.

Hint 56

Hint = Caveman VP produces fire.

Front 57

What length of time is required for the destruction of S. aureua? B. megaterium? Why does it take longer for B. megaterium?

Back 57

S. aureus: 10 minutes
S. aureus: 10 minutes
S. aureus: 10 minutes
S. aureus: 10 minutes
B. megaterium: 60 min.
because of endospores.

Front 58

Why leave the cover on one set of petri dishes when exposing to US light?

Back 58

To use as a control.

Front 59

Describe what happens when DNA is exposed to UV light?

Back 59

Thymines cross-link to form thymine dymers.

Front 60

How do cells repair UV damage?

Back 60

SOS: enymatically rfemoves the dimers and inserts pyrimidine molecules in their place. It cannot repair all damage. It makes errors by inserting bases.

Front 61

Differentiate between antimicrobials and antibiotics.

Back 61

Antimicrobials
kill/control bacterial "GROWTH".
Antibiotics
made from other organisms damage the CELL WALL of bacteria.

Front 62

A narrow spectrum antimicrobe ___ ___.

Back 62

controls few.

Front 63

A broad spectrum antimicrobic.

Back 63

Controls many.

Front 64

Which antiseptics were most effective at controlling the growth of S. aureus and P. aeruginosa?

Back 64

Formaldehyde for both.

Front 65

What factors influence the zone of inhibition?

Back 65

amount of exposure and sample.

Front 66

Differentiate between antiseptics and disinfectants.

Back 66

Antiseptics
are gentle enough to use on living tissue but don't destroy endospores.
Disinfectants
are used on inanimate objects and damage tissues.
You disinfect inanimate objects
NOT PEOPLE.

Front 67

Differentiate between bacteriostatic and bacteriocidal.

Back 67

Bacteriostatic are agents inhibit bacterial growth.
bacteriocidal are agents that kill bacteria.

Front 68

An excellent "broad"-spectrum antimicrobic.

Back 68

Gentamycin.

Hint 68

hint= Gentlemen look at broads.

Front 69

Loves sugar.

Back 69

Osmophile.

Front 70

This microorganism tolerates a broad range of salt concentrations.

Back 70

Staph-ylo-coccus

Front 71

What is the optimum temperature for E.coli?

Back 71

38-42 degrees C.